The effects of cyclic adenosine monophosphate, forskolin, and theophylline on motility parameters in gossypol-treated human sperm

Cowart, Catherine L.; London, Steve N.; Vernon, Michael; Pedigo, Nancy G.

doi:10.1016/s0015-0282(16)56708-9

Cited by 10 publications

(6 citation statements)

References 25 publications

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“…Gossypol did not affect fertilizing ability of sperm even at 50 jig/ml. Possibly, effects of gossypol on fertilizing ability were obscured by the high sperm:oocyte ratio used in IVE In other studies, 9.3-10 jig/ml gossypol caused a slight reduction in motility of ram [6] and human sperm [7]. Differences between these studies and the present ones may reflect differences between species or methods for assessing motility.…”

Section: Discussioncontrasting

confidence: 60%

“…Feeding cottonseed high in gossypol content to bulls has been associated with reduced sperm production, increased sperm abnormalities, decreased sperm motility, and decreased thickness of the germinal epithelium of the testis [2,3]. Gossypol has been reported to compromise function of cultured spermatozoa from cattle [4,5] and other species [6,7]. Exposure to gossypol while in the female reproductive tract could conceivably result in damage to sperm.…”

Section: Introductionmentioning

confidence: 99%

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Deleterious Actions of Gossypol on Bovine Spermatozoa, Oocytes, and Embryos1

Brocas

Rivera

Paula-Lopes

et al. 1997

Biology of Reproduction

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Gossypol (50 and 100 micrograms/ml) decreased the percentage of sperm that completed the swim-up procedure. This effect was not blocked by glutathione monoethyl ester. Cleavage rates were not different between oocytes inseminated with gossypol-treated spermatozoa (10 or 50 micrograms/ml) and oocytes inseminated with control spermatozoa. Development to the blastocyst stage at Day 7 after insemination was reduced when spermatozoa treated with 50 micrograms/ml gossypol were used for fertilization. Gossypol toxicity was evident in cows fed cottonseed meal because erythrocyte fragility was greater than for control cows. However, there were no differences between cottonseed meal and control groups in number of oocytes collected per cow, cleavage rate after in vitro maturation and fertilization, or the proportion of oocytes or embryos that developed to blastocysts. Similarly, exposure of oocytes to 2.5-10 micrograms/ml gossypol during in vitro maturation did not affect cleavage rates or subsequent development. In contrast, addition of 10 micrograms/ml gossypol to embryos reduced cleavage rate. Moreover, development of cleaved embryos was reduced by culture with 5 or 10 micrograms/ml gossypol and tended to be reduced by 2.5 micrograms/ml gossypol. In conclusion, bovine gametes are resistant to gossypol at concentrations similar to those in blood of cows fed cottonseed meal. In contrast, the developing embryo is sensitive to gossypol.

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Section: Discussioncontrasting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Deleterious Actions of Gossypol on Bovine Spermatozoa, Oocytes, and Embryos1

Brocas

Rivera

Paula-Lopes

et al. 1997

Biology of Reproduction

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show abstract

“…Theophylline enhances sperm motility by increasing cAMP level into sperm (3,14) .In this study, the mean of progressive sperm motility (A+B) was below fifty percent for both of healthy subjects and asthenozoospermic male patients before activation (raw semen) increased to exceed 50% (within fertile values according to (WHO,1999)) just only after activation by SpermMobil dilution.…”

Section: Discussion:-mentioning

confidence: 45%

A Stimulatory Approach for in Vitro Activation of Human Sperm Motility by Using Theophylline.

Abbas¹,

Technologies²,

Mossa.³

et al. 2018

IJAR

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“…Concentrations of methyl xanthines reported in our work were similar to those used for enhancement of sperm quality. For example, enhancement of semen characteristics has been reported at concentrations of caffeine and pentoxifylline up to 20 mM (Stachecki et al, 1994), and theophylline has been used at concentrations of 10 mM and 20 mM (Loughlin and Agarwal, 1992;Cowart et al, 1994) or even 30 mM (Jaiswal and Majumder, 1996). If sperm alkaline phosphatase of other mammalian species is similarly inhibited by methyl xanthines, as we have demonstrated for boar alkaline phosphatase in the present study, these data suggest that beneficial effects of these substances may be partially related to their action on sperm alkaline phosphatase.…”

Section: Discussionmentioning

confidence: 99%

“…In numerous studies, methyl xanthine supplementation resulted in better motility characteristics in fresh and cryopreserved spermatozoa. These include a greater percentage of motile cells and greater velocity of sperm movement, including curvilinear velocity, which often leads to a higher percentage of nonhyperactive sperm shifting to either a transitional or hyperactivated state (Tash and Means, 1983;Schoff and Lardy, 1987;Rees et al, 1990;Sikka and Hellstrom 1991;Cowart et al, 1994;Lewis et al, 1994;Koutsarova et al, 1997;Merino et al, 1997;Sharma and Agarwal, 1997;Nassar et al, 1999a;Ponce et al, 1999).…”

mentioning

confidence: 99%

Inhibition of Alkaline Phosphatase Activity of Boar Semen by Pentoxifylline, Caffeine, and Theophylline

GLOGOWSKI,

DANFORTH,

CIERESZKO

2002

Journal of Andrology

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Methyl xanthines have been used frequently as additives to sperm suspensions in order to improve sperm characteristics. The mechanism of action on spermatozoa is generally assumed to be inhibition of sperm phosphodiesterase activity, resulting in elevation of complementary adenosine monophosphate levels in spermatozoa. The present study was designed to examine the effect of methyl xanthines (pentoxifylline, caffeine, and theophylline) on another important enzyme system, alkaline phosphatase, in boar seminal plasma and spermatozoa. Inhibition of sperm alkaline phosphatase could be distinguished from that of seminal plasma by a paradoxical stimulation by pentoxifylline at lower pH values in spermatozoa. Among the three methyl xanthines, theophylline exhibited the most dramatic inhibition of alkaline phosphatase activity and substrate inhibition was observed with increasing concentrations. Each methyl xanthine had a different action on alkaline phosphatase activity at lower pH; theophylline showed the highest inhibition, caffeine inhibition was not related to pH, and pentoxifylline did not inhibit alkaline phosphatase of seminal plasma and, in fact, it stimulated its activity (or that of a phosphatase with lower pH optimum) in spermatozoa. These results indicate another possible mechanism of action of methyl xanthines on sperm and are in agreement with data indicating that methyl xanthines are not specific inhibitors of sperm phosphodiesterase, because clearly, they inhibit alkaline phosphatase activity as well.

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The effects of cyclic adenosine monophosphate, forskolin, and theophylline on motility parameters in gossypol-treated human sperm

Cited by 10 publications

References 25 publications

Deleterious Actions of Gossypol on Bovine Spermatozoa, Oocytes, and Embryos1

Deleterious Actions of Gossypol on Bovine Spermatozoa, Oocytes, and Embryos1

A Stimulatory Approach for in Vitro Activation of Human Sperm Motility by Using Theophylline.

Inhibition of Alkaline Phosphatase Activity of Boar Semen by Pentoxifylline, Caffeine, and Theophylline

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