The Effects of Cytotoxic Dose of Diclofenac on CD82/KAI1 in Cervical Cancer Cells in Cell Culture

Abstract

In this laboratory experimental study we assessed antimetastasis effects of Diclofenac on cervical cancer cells in cell culture. For this purpose, the cells (Hela cell line) were used in our study. MTT assay was used to determine cytotoxic effects of diclofenac followed by Real Time PCR to assay KAI gene expression in cells in which the extract have shown cytotoxic effects. Our results showed that administration of diclofenac (0.1 mg/ml) led to decrease in expression of antimetastasis KAI gene indicating "anti-antimetastasis" effects of diclofenac on cervical cancer cells in cell culture.