The Effects of Different Concentrations Cytokinins on the in vitro Multiplication of Plantain (Musa sp.)

Buah, J. N.; Danso, E.; Taah, Kingsley J.; Abole, E. A.; Bediako, E. A.; Asiedu, J.B.K.; Baidoo, Richard

doi:10.3923/biotech.2010.343.347

Cited by 68 publications

(56 citation statements)

References 8 publications

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“…It is also possible that the amount of BA conjugated to medium was less compared to other hormones. As a result, large amount of BA exist as free or severable forms, which are readily (Buah et al 2010).…”

Section: Resultsmentioning

confidence: 99%

Micropropagation of annatto (Bixa orellana L.) from mature tree and assessment of genetic fidelity of micropropagated plants with RAPD markers

Siril

Joseph

2012

Physiol Mol Biol Plants

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An in vitro propagation technique based on axillary bud proliferation was developed for the first time to mature annatto (Bixa orellana L.) tree. Nodal segments cultured on Murashige and Skoog (MS) medium supplemented with 1.0 μM benzyl adenine (BA) and tender coconut water (10 %) showed significantly high (P < 0.05) explant response (67.0 %), development of elongated shoots (3.36), shoot buds (8.9) and shoot elongation (3.53 cm). Cytokinins like zeatin, isopentenyl adenine (2-iP), kinetin, or thidiazuron (TDZ) were inferior to BA to induce multiple shoots. Seasonal variations significantly affected the in vitro response of nodal explants. In vitro rooting experiments have showed 55.6 % rooting on MS medium containing 15 μM indole-3-butyric acid (IBA). Alternatively, in vitro raised shoots were rooted (61.1 %) ex vitro, by 10 mM indole-3-butyric acid (IBA) for 30 s. The results of the RAPD marker system revealed the genetic stability among the micropropagated plants. The present protocol in brief, can be used for the clonal propagation of the superior genotype and preservation of germplasm.

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Section: Resultsmentioning

confidence: 99%

Micropropagation of annatto (Bixa orellana L.) from mature tree and assessment of genetic fidelity of micropropagated plants with RAPD markers

Siril

Joseph

2012

Physiol Mol Biol Plants

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“…The sheaths that formed the pseudostem were carefully removed to reduce the size of the material to about four leaf sheaths. They were then sterilized with 70% ethanol for 3 min and washed three times in sterilized distilled water (Buah et al, 2010). More leaf sheaths were then removed aseptically in a clean bench until about two leaves covered the shoot meristem.…”

Section: Materials and Methods Plant Materials And Explant Preparationmentioning

confidence: 99%

“…Thirty grams (30 g LG 1 ) sucrose and 6 g LG 1 agar were used because they had been the optimal concentrations from previous work with Musa species (Buah et al, 2010). The pH of the media was adjusted at 5.8 with HCl and NaOH before autoclaving for 15 min at 121°C.…”

Section: Media Compositionmentioning

confidence: 99%

In vitro Growth of Dwarf Cavendish Banana Plantlets in Different Culture Vessels and Light Intensities

Buah¹

2015

International J. of Agricultural Research

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The growth and multiplication of a local Cavendish banana called "Kwadu Pa" was investigated under three different light intensities and three types of culture vessels. The light intensities were 4000, 5000 and 6000 Lux with glass, plastic bottles and conical flask as the vessel types. Each of the treatments for light intensity and vessel type were replicated 50 times and cultures were arranged in a completely randomized fashion. They were cultured for 16 weeks and data was taken on mean shoot fresh weight, number of shoots per plantlet, shoot dry weight, leaf chlorophyll content and shoot height. Plantlets that were cultured in conical flask under the three different light intensities had higher values for all the parameters measured under the different light intensities. Among the light intensities, plantlets that were cultured under 5000 Lux showed superior growth and multiplication compared to those cultured under 4000 and 6000 Lux. At light intensities above 5000 Lux, growth characteristics and chlorophyll content declined significantly. Conical flask as a culture vessel and photon flux of 5000 Lux were optimal for the in vitro growth and multiplication of banana.

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“…It is also possible that the amount of BA that got conjugated in the medium was smaller than what happened to the other plant hormones. This would then have larger amount of BA existing in their free or ionized forms and were readily made available to plant tissues from the medium (Buah, et al, 2010). Generally, BA was more effective on shoot formation than Kin in guava, indicating cytokinin specificity for shoot induction in its tissue.…”

Section: Shoot Multiplicationmentioning

confidence: 99%

Micropropagation of guava (Psidium guajava L.)

Hassanen¹,

Daib²,

Omar³

2017

IOSR JAVS

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The Effects of Different Concentrations Cytokinins on the in vitro Multiplication of Plantain (Musa sp.)

Cited by 68 publications

References 8 publications

Micropropagation of annatto (Bixa orellana L.) from mature tree and assessment of genetic fidelity of micropropagated plants with RAPD markers

Micropropagation of annatto (Bixa orellana L.) from mature tree and assessment of genetic fidelity of micropropagated plants with RAPD markers

In vitro Growth of Dwarf Cavendish Banana Plantlets in Different Culture Vessels and Light Intensities

Micropropagation of guava (Psidium guajava L.)

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