The Effects of Different Concentrations of Nano-Silver on Elimination of Bacterial Contaminations and Phenolic Exudation of Rose (Rosa Hybrida L.) in Vitro Culture

Abstract

Rose is known as the first and the most important cutflowers in all over the world. One of the common methods of mass production of this plant is propagation through tissue culture. The main limiting factor of rose tissue culture is Bacterial contaminations and phenolic exudation in the establishment phase that significantly makes most of explants spoiled. Nano-Silver is able to control and stop the bacterial contamination. In this experiment Nano-Silver was added to medium with concentrations of (, , and ppm) and in the other experiments explants were immersed in solutions of Nano-Silver with concentrations (, , and ppm) in complete random design. The experiments were carried out with four replications. The results showed that the concentration of ppm which is directly added to the medium can reduce Bacterial contamination and phenolic exudation rate. Concentration of ppm for minutes after surface sterilization was the best treatment of immersion to control bacterial contaminations. High concentrations of Nano-Silver make regeneration of explants more and more slower and in some cases lead to destroy explants. In general, Nano-Silver had no effects on fungal contamination.

Artificial Neural Network Genetic Algorithm As Powerful Tool to Predict and Optimize In vitro Proliferation Mineral Medium for G × N15 Rootstock

“…Shoot proliferation is influenced by several factors, such as genotype, media composition (Ruzic and Vujovic, 2008; Ivanova and Van Staden, 2009; Yang et al, 2012), disinfection and establishment methods (Shokri et al, 2013; Arab et al, 2014), in vitro environmental factors, etc. Development of an appropriate culture medium for a specific crop can be quite complex because the response to the culture medium is often genotype dependent, and the effects of mineral nutrition on morphogenesis have hardly been studied (Ramage and Williams, 2002; George et al, 2008; Greenway et al, 2012; Wada et al, 2013).…”

The Effects Nano-Silver on Contamination of Spring Barley ‘Eunova’ in Vitro

“…While addition to the MS medium 400 mg • dm -3 of nano-silver decreased the number of bacterial contaminations from 81.25% to 18.75%. Shokri et al (2014) showed, that adds to the MS medium 100 ppm of nano-silver decreased the number of bacterial contamination from 70% to 17.5%, and 87.5% rose (Rosa hybryda L.) explants were able to developed shoots. In addition, submerging of rose explants in 200 ppm of nano-silver solution for 20 minutes showed the best disinfection efficiency.…”

“…In addition, submerging of rose explants in 200 ppm of nano-silver solution for 20 minutes showed the best disinfection efficiency. According to Shokri et al (2014) adding nano-silver to the MS medium allowed to obtain the highest efficiency of disinfection with the higher explants regeneration rate with a comparison to the submerging method. In the present study, we demonstrated the influence of nano-silver on the morphological traits of spring barley (Table 2).…”

“…However, the success of micropropagation is determined by many factors. One of them is the effective removal of exogenous and endogenous microorganisms (Shokri et al 2014). The most common pathogens infecting plant cultures are bacteria, fungi and even yeast (Abdi et al 2008).…”

“…Nano-silver is a non-toxic molecule of size less than 100 nm, characterized by high efficiency in eliminating bacterial infections (Abdi et al 2008;Rostami and Shahsavar 2009;Sarmast et al 2011;Shokri et al 2014). Silver ions interact with many processes at the molecular level in bacterial cells, resulting in the inhibition of their growth and even death (Abdi et al 2008).…”