The effects of different hygiene procedures in reducing bacterial contamination in a model domestic kitchen

Røssvoll, Elin; Langsrud, Solveig; Bloomfield, Sally F.; Moen, Birgitte; Heir, Even; Møretrø, Trond

doi:10.1111/jam.12869

Cited by 29 publications

(33 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The swabbing and plating were as described previously (Røssvoll et al 2015). Briefly, 2 swabs were used for each coupon-sink area and both swabs were put in the same tube with 3 mL of Dey-Engley (D/E) neutralizing broth (BD Difco, New Jersey, USA), and serial 10-fold dilutions were prepared in PBS and spiral plated on tryptic soy agar (TSA; Oxoid, Basingstoke, UK).…”

Section: Surface Sampling and Cultivation Methodsmentioning

confidence: 99%

“…The experimental setup of the steel coupons in the domestic kitchen sinks (stainless steel) has previously been described (Røssvoll et al 2015). Briefly, 8 volunteers attached 3 new stainless steel coupons (AISI 304, 2B; Norsk Stål AS, Nesbru, Norway) in their kitchen sinks in January 2013.…”

Section: Experimental Designmentioning

confidence: 99%

“…PCR and sequencing was performed as described previously (Røssvoll et al 2015). Briefly, universal primers (Nadkarni et al 2002) were used for 16S rRNA gene amplification (V3-V4) and sequencing.…”

Section: Pcr and Sequencing Of Coloniesmentioning

confidence: 99%

“…Ideally, this will allow studies on biofilms that are more relevant than those produced using laboratory models. The approach has been used to compare hygienic properties of different materials (Guobjornsdottir et al 2005), to identify microbiota in food production factories (Hood and Zottola 1997;Mettler and Carpentier 1998;Gunduz and Tuncel 2006), and to detect biofilm formation (Holah et al 1989;Gibson et al 1995), and recently, we used this approach to study the effect of kitchen cleaning methods (Røssvoll et al 2015). However, little has been done to evaluate how well the microbiota developed by this approach reflects the microbiota developed in situ, where the surfaces can be of a different quality and (or) condition than the coupons used.…”

Section: Introductionmentioning

confidence: 99%

“…The samples were collected in a parallel study previously published (Røssvoll et al 2015). Domestic sinks were chosen as a suitable environment, since their material (stainless steel) is comparable to that of the coupons and since they are heavily exposed to and colonized by bacteria (e.g., from raw produce, water, and skin microbes).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Microbiota formed on attached stainless steel coupons correlates with the natural biofilm of the sink surface in domestic kitchens

et al. 2016

View full text Add to dashboard Cite

Stainless steel coupons are frequently used in biofilm studies in the laboratory, as this material is commonly used in the food industry. The coupons are attached to different surfaces to create a "natural" biofilm to be studied further in laboratory trials. However, little has been done to investigate how well the microbiota on such coupons represents the surrounding environment. The microbiota on sink wall surfaces and on new stainless steel coupons attached to the sink wall for 3 months in 8 domestic kitchen sinks was investigated by nextgeneration sequencing (MiSeq) of the 16S rRNA gene derived from DNA and RNA (cDNA), and by plating and identification of colonies. The mean number of colony-forming units was about 10-fold higher for coupons than sink surfaces, and more variation in bacterial counts between kitchens was seen on sink surfaces than coupons. The microbiota in the majority of biofilms was dominated by Moraxellaceae (genus Moraxella/Enhydrobacter) and Micrococcaceae (genus Kocuria). The results demonstrated that the variation in the microbiota was mainly due to differences between kitchens (38.2%), followed by the different nucleic acid template (DNA vs RNA) (10.8%), and that only 5.1% of the variation was a result of differences between coupons and sink surfaces. The microbiota variation between sink surfaces and coupons was smaller for samples based on their RNA than on their DNA. Overall, our results suggest that new stainless steel coupons are suited to model the dominating part of the natural microbiota of the surrounding environment and, furthermore, are suitable for different downstream studies.Key words: microbiota, stainless steel coupons, sink surface, domestic kitchens. Résumé :Les études sur les biofilms utilisent couramment des coupons en acier inoxydable en laboratoire puisque ce matériau est répandu dans l'industrie alimentaire. Ces coupons sont attachés à diverses surfaces afin de créer un biofilm « naturel » aux fins d'études plus approfondies en laboratoire. Or, on s'est peu demandé à quel point la microflore retrouvée sur de tels coupons est représentative de l'environnement avoisinant. On a examiné la microflore de nouveaux coupons d'acier inoxydable attachés à la paroi de l'évier pendant trois mois, ainsi que celle des parois de l'évier comme telles, au moyen du séquençage de prochaine génération (MiSeq) du gène de l'ARNr 16S issu d'ADN et d'ARN (ADNc), et par ensemencement et identification des colonies. Le nombre moyen d'unités formant colonies était environ 10 fois plus élevé sur les coupons que sur les surfaces de l'évier, et on a observé davantage de variations des numérations bactériennes selon les cuisines dans le contexte des surfaces d'éviers que des coupons. La microflore de la majorité des biofilms était dominée par les Moraxellaceae (genre Moraxella/ Enhydrobacter) et les Micrococcaceae (genre Kocuria). Les résultats ont démontré que la variation de la microflore était principalement attribuable aux différences entre les cuisines (38,2 %), suivie par les ...

show abstract

Section: Surface Sampling and Cultivation Methodsmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Section: Pcr and Sequencing Of Coloniesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Microbiota formed on attached stainless steel coupons correlates with the natural biofilm of the sink surface in domestic kitchens

et al. 2016

View full text Add to dashboard Cite

show abstract

Trends in Polymer Degradation Across All Scales

Veskova

Sbordone

Frisch

2022

Macro Chemistry & Physics

View full text Add to dashboard Cite

The development of sustainable plastic materials will be flanked with conscious resource management, waste recovery frameworks, and social change. Nonetheless, developing strategies toward controlled polymer degradation remains a key challenge—whether as a failsafe mechanism for materials that escape the resource recovery cycle, or where distinct degradation pathways are required for specific applications such as in the biomedical realm. This perspective highlights recent trends, challenges, and future strategies on three levels: 1) On the materials level, by the incorporation of enzymes into polymer materials that catalyze polymer degradation under benign conditions; 2) On the domain level, crystalline segments of polymer materials are often inert, even to enzymatically catalyzed degradation. Gaining an understanding of the mode of interaction between enzymes and polymer chains is key to controlling degradation of all polymer morphologies within materials. Processive depolymerization mechanisms, where the enzyme binds polymer chain ends and depolymerizes along the chain are extremely promising for efficient polymer degradation; 3) On the molecular level, where polyesters exhibit enzymatic targets of ester bonds through their polymer backbone, poly(alkene)s c of all carbon backbones. To enable degradation of this most abundant class of polymers, strategies must be developed to incorporate enzymatic targets into the backbone.

show abstract

Quantitative risk assessment for bacterial community in residential kitchens

Liu

et al. 2023

Building and Environment

View full text Add to dashboard Cite

The effects of different hygiene procedures in reducing bacterial contamination in a model domestic kitchen

Cited by 29 publications

References 41 publications

Microbiota formed on attached stainless steel coupons correlates with the natural biofilm of the sink surface in domestic kitchens

Microbiota formed on attached stainless steel coupons correlates with the natural biofilm of the sink surface in domestic kitchens

Trends in Polymer Degradation Across All Scales

Quantitative risk assessment for bacterial community in residential kitchens

Contact Info

Product

Resources

About