The Effects of Different Media on Shoot Proliferation From the Shoot Tip of Aloe vera L.

Abstract

Background: Aloe vera L. is an important pharmaceutical plant from which several medicinal and cosmetic compounds are extracted. Aloe is naturally propagated through offset, which is a slow and expensive labor cost method with low economical income. Objectives: In this study, the effect of different media on shoot proliferation of the shoot tip of Aloe vera L. was investigated. Materials and Methods:In vitro techniques are some of the suggested methods for rapid propagation of Aloe. In this experiment, the shoot tips of mother plants were grown in a greenhouse. After surface sterilization of the explants, they were cultured on Murashige and Skoog (1962) (MS) medium containing different concentrations of kinetin and naphthaleneacetic acid (NAA). The experiment was carried out in the form of a randomized complete design with three replications. Results:The results showed that MS media containing 1.5 mg/L kinetin along with 0.15 or 0.3 mg/L NAA produced the highest percentage of proliferated shoots. In addition, the percentage of proliferated shoots in MS medium containing 2.0 or 2.5 mg/L benzylaminopurine (BAP) + 0.15 mg/L NAA was significantly higher than the other treatments. Conclusions: Analysis of the interactive effects of NAA, kinetin and BAP on shoot proliferation showed that most of the proliferated shoots produced in MS medium containing 1.0 mg/L BAP + 1.0 mg/L kinetin + 0.15 mg/L NAA were significantly different from other treatments. Rooting quality was greater in MS media containing 1.0 mg/L IBA than a 1.0 mg/L NAA treatment.

In VitroMicropropagation ofAloe adigratanaReynolds Using Offshoot Cuttings

“…Although the statistical differences among the treatments are not profound, IBA yielded better results in terms of mean root length. Similar findings were reported by many other researchers [13,25,32,45]. On the other hand, the mean root length increased with increasing the concentration of IBA from 0.5 to 1.5 mg/L, while the reverse was true with NAA.…”

“…NAA resulted in better effects in lower concentrations (0.5 and 1.0 mg/L) than in higher concentration (1.5 mg/L). Similar patterns were reported with A. elegans [21], A. trichosantha [22], A. percrassa [15], A. barbadensis [23], A. vera [32], and A. polyphylla [43]. Other workers also reported better rooting responses with NAA at 0.5 mg/L [26,42,44].…”

“…Many researchers reported varied shooting responses with A. vera. For example, Daneshvar et al [32] observed the highest shoot proliferation (28. [25,35]. is difference may be due to the type of explant and genotypic difference that often affect tissue culture response.…”

A Review on in vitro Culture of Aloe vera, Type of Explants and Impact of Growing Media and Growth Regulators

“…After 7 weeks of inoculation, greatest number of shoots (11.18) and highest shoot length (12.15 cm) was achieved. Daneshvar et al, (2013) studied the effect of cytokinins on shoot proliferation of Aloe vera. In media free of cytokinin, the explants produced mostly callus and/or a single shoot along with rhizogenesis.…”

Auxin and cytokinin synergism in micropropagation for mass production of Aloe vera

“…Interestingly, 6-benzylaminopurine (BAP) alone was proved to be more effective than the combination of BAP and naphthaleneacetic acid (NAA) for in vitro propagation of A. vera (Danial et al, 2019 ). In contrast, combinations of PGRs (BAP and NAA) were also shown to be effective for optimal in vitro shoot proliferation in A. vera and Aloe adigratana Reynolds (Daneshvar et al, 2013 ; Niguse et al, 2020 ). Moreover, the maximum shoot bud induction in A. vera was achieved with a combination of BAP and NAA (Jakhar et al, 2020 ).…”