The Effects of Diurnal Intermittent Fasting On Proinflammatory Cytokine, Oxidative Stress and Heat Shock Protein 70 Levels

Abstract

In this study, it is aimed to assess the effect of intermittent diurnal fasting (DIF) at the beginning (2nd day of Ramadan fasting) and the end of the Ramadan (28th day of Ramadan fasting) on plasma levels of proinflammatory cytokines [tumor necrosis factor-alpha (TNF-α) and interleukin 8 (IL-8)], heat shock protein 70 (Hsp70) and oxidative stress markers [total oxidant status (TOS) and total antioxidant status (TAS)]. Methods: Fifty healthy male volunteers aged between 25-45 years were included in the study. All subjects followed the same dietary regimen before and during Ramadan and were encouraged to continue their usual lifestyle and activities. All biochemical tests were made both at the beginning (2nd day of fasting) and the end of the Ramadan (28th day of fasting). Plasma levels of cytokines (TNF-α and IL-8) Hsp70 were assessed using enzyme-linked immunoassays (ELISA), and oxidative markers (TAS and TOS) were measured spectrophotometric methods. Results: There was a significant decrease in the levels of TNF-α, compared to the beginning of fasting (p<0.05). However, there was no significant change in IL-8 levels between before and end of fasting (p>0.05). Therefore, there was a significant increase in the Hsp70 levels compared to the beginning of fasting (p<0.05). On the other hand, TOS levels reduced at the end of fasting compared to the beginning (p<0.05). In addition, TAS levels were similar and looked unchanged during the period of fasting (p>0.05). Conclusion: DIF decreased proinflammatory cytokine level and oxidative stress parameter while increasing the levels of Hsp70.

The Effect of Intermittent Fasting and Exercise on Body Composition, Antioxidant Capacity, and Blood Parameters in Obese Rats

“…In contrast, TOS and OSI were found to be significantly lower compared to non-fasting people [29]. Conversely, a significant decrease was observed among TOS measured at the beginning of Ramadan and TOS measured at the end of Ramadan, while no change was observed in TAS values [30]. Some studies analyzed different oxidative stress parameters with intermittent fasting.…”