The effects of drought on the expression of TAO1, NCED and EIL1 genes and ABA content in tomato wild-type and flacca mutant

Milosavljević, Aleksandra; Prokić, Ljiljana; Marjanovic, Milena; Stikić, R.; Sabovljević, Aneta

doi:10.2298/abs1201297m

Cited by 7 publications

(6 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our result on the up-regulation of the NCED1 gene under water deprivation is in line with the observed ABA accumulation in tomato leaves (Figure 6). The up-regulation of NCEDs genes under drought has been demonstrated elsewhere [61,62,65,66]. We assume that the more pronounced expression of the NCED1 gene in WT observed in the 3rd drought cycle was mostly due to its de novo biosynthesis, while in the 1st and 2nd cycles the ABA was synthesized from its precursors and conjugates.…”

Section: Low Aba Differences Results In Flacca Traitsmentioning

confidence: 68%

Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles

Živanović

Komić

Nikolić

et al. 2021

Plants

View full text Add to dashboard Cite

Two tomato genotypes with constitutively different ABA level, flacca mutant and wild type of Ailsa Craig cv. (WT), were subjected to three repeated drought cycles, with the aim to reveal the role of the abscisic acid (ABA) threshold in developing drought tolerance. Differential responses to drought of two genotypes were obtained: more pronounced stomatal closure, ABA biosynthesis and proline accumulation in WT compared to the mutant were compensated by dry weight accumulation accompanied by transient redox disbalance in flacca. Fourier-transform infrared (FTIR) spectra analysis of isolated cell wall material and morphological parameter measurements on tomato leaves indicated changes in dry weight accumulation and carbon re-allocation to cell wall constituents in flacca, but not in WT. A higher proportion of cellulose, pectin and lignin in isolated cell walls from flacca leaves further increased with repeated drought cycles. Different ABA-dependent stomatal closure between drought cycles implies that acquisition of stomatal sensitivity may be a part of stress memory mechanism developed under given conditions. The regulatory role of ABA in the cell wall restructuring and growth regulation under low leaf potential was discussed with emphasis on the beneficial effects of drought priming in developing differential defense strategies against drought.

show abstract

Section: Low Aba Differences Results In Flacca Traitsmentioning

confidence: 68%

Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles

Živanović

Komić

Nikolić

et al. 2021

Plants

View full text Add to dashboard Cite

show abstract

“…Our results showed that drought-induced accumulation of ABA in the leaves was more pronounced in wild type compared to flacca mutant (Table 4) that could be explained a specific stomata and leaf response. Investigation of gene expression in Ailsa Craig leaves indicate a synergistic effect of signaling pathways for ABA and ethylene, while in flacca the expression of the NCED gene was reduced and EIL1 was increased, which indirectly imply an increase in the concentration of ethylene [30]. Therefore, the explanation for the smaller leaf area and mass of flacca mutant or specific leaf response compared to the wild type may be due to decreased ABA production or increased ethylene concentration.…”

Section: Biochemical and Metabolic Processesmentioning

confidence: 99%

Influence of Severe Drought on Leaf Response in ABA Contrasting Tomato Genotypes (Wild Type and flacca Mutant)

Petrović

Jovanović

Stikić

et al. 2020

The 1st International Electronic Conference on Plant Science

View full text Add to dashboard Cite

The reaction of leaf growth to drought stress is controlled by various hormones, among which ABA is one of the most important. The aim of this study was to examine the effects of ABA deficiency on tomato leaf response under severe drought stress. Therefore, ABA-mutant (flacca) and wild type (Ailsa Craig) were selected for research and in the stage of second flower truss anthesis plants were exposed to severe water deficit. The effects of severe drought on wild-type leaves and flacca mutant showed that, as a result of reduced ABA concentrations, flacca plants were exposed to a higher degree of stress than wild-type leaves, which had a negative impact on the examined physiological and biochemical parameters. Severe drought caused stomatal closure, decreased water potential, specific leaf area, and chlorophyll concentrations in the leaves in both genotypes, but this was more pronounced in the mutant. Wild-type plants have accumulated more vitamin C and ABA and have a higher total antioxidant capacity in the leaves in dry conditions than flacca mutants, which contribute to their better adaptive response to stress. Based on this, it can be assumed that the ABA mutation has led to a decrease in the capacity for oxidative stress products caused by severe drought stress.

show abstract

“…Tomato (Solanum lycopersicum) plants exposed to DI and PRD showed no consistent changes in the expression of genes related to ABA biosynthesis (SlTAO1 and SlNCED) and ethylene sensitivity (SlEIL1; [18]), and these changes were not consistently related to soil or plant water status.…”

Section: Introductionmentioning

confidence: 99%

Physiological and gene expression responses of sunflower (Helianthus annuus L.) plants differ according to irrigation placement

et al. 2014

View full text Add to dashboard Cite

To investigate effects of soil moisture heterogeneity on shoot physiology and root and foliar gene expression, three treatments were implemented in sunflower plants growing with roots split between two compartments: a control (C) treatment supplying 100% of plant evapotranspiration, and two treatments receiving 50% of plant evapotranspiration, either evenly distributed to both compartments (deficit irrigation-DI) or unevenly distributed to ensure distinct wet and dry compartments (partial rootzone drying-PRD). After 3 days, evapotranspiration was similar in C and DI, but 20% less in PRD, concomitant with decreased leaf water potential (Ψ leaf) and increased leaf xylem ABA concentration. Six water-stress responsive genes were highly induced in roots of PRD plants exposed to drying soil, but not in roots of DI plants exposed to higher soil moisture content. In leaves of PRD plants, gene expression was correlated with increased xylem ABA concentration and decreased Ψ leaf. PRD triggered stronger physiological and molecular responses than DI in roots and leaves suggesting a more intense and systemic stress response due to local dehydration of the dry compartment. Physiological vs. molecular correlation studies in PRD/DI plants provide insights into the severity and location of water deficit and may enable a better understanding of longdistance signalling mechanisms.

show abstract

The effects of drought on the expression of TAO1, NCED and EIL1 genes and ABA content in tomato wild-type and flacca mutant

Cited by 7 publications

References 28 publications

Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles

Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles

Influence of Severe Drought on Leaf Response in ABA Contrasting Tomato Genotypes (Wild Type and flacca Mutant)

Physiological and gene expression responses of sunflower (Helianthus annuus L.) plants differ according to irrigation placement

Contact Info

Product

Resources

About