The effects of electrospun TSF nanofiber diameter and alignment on neuronal differentiation of human embryonic stem cells

Wang, Junxia; Ye, Rong; Wei, Youhua; Xu, Xiaojing; Zhang, Feng; Qu, Jing; Zuo, Baoqi; Zhang, Huanxiang

doi:10.1002/jbm.a.33291

Cited by 99 publications

(83 citation statements)

References 68 publications

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“…As dictated in previous studies on neural and vascular cells (Daud et al 2012;Wang et al 2012;Christopherson et al 2009), fibre diameter does affect the viability of cells. Similarly to our results Christopherson et al (2009) and Daud et al (2012) found that when fibre diameter is around 1-1.5 µm cells have a lower proliferative capacity.…”

Section: Discussionmentioning

confidence: 66%

“…These experiments using electrospun scaffolds did not consider the mechanical or physical properties of the scaffolds and the effect this has on the cells. It is well documented that changes is fibre diameter and orientation induce a response of the cells in many tissue types (Yan et al 2012;Balguid et al 2009;Wang et al 2012), but the effect on renal cells has been ignored until now. The diameter of the fibre determines the pore size of the scaffold, and this determines how well cells will penetrate into a scaffold (Balguid et al 2009;Baker et al 2008;Ju et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…This variation in morphology has been shown to affect the way cells behave and integrate with the scaffold, larger fibres allow for greater cell integration, nanofibres represent the natural ECM and aligned fibres have shown to guide neural, vascular and cornea cell growth (Yan et al 2012;Balguid et al 2009;Wang et al 2012). Other methods have been proposed to increase the porosity of scaffolds to allow for greater cell integration; dual-spinning using a water soluble sacrificial material allows for a scaffold with greater porosity (Lowery et al 2010;Phipps et al 2012) and techniques such as cell electrospinning enable cell to be directly integrated within the scaffold (Jayasinghe 2013;Townsend-Nicholson & Jayasinghe 2006).…”

Section: Introductionmentioning

confidence: 99%

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The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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There is a pressing need for further advancement in tissue engineering of functional organs with a view to providing a more clinically relevant model for drug development and reduce the dependence on organ donation. Polymer based scaffolds, such as polycaprolactone (PCL), have been highlighted as a potential avenue for tissue engineered kidneys, but there is little investigation down this stream.Focus within kidney tissue engineering has been on 2-dimensional cell culture and decellularised tissue. Electrospun polymer scaffolds can be created with a variety of fibre diameters and have shown a great potential in many areas. The variation in morphology of tissue engineering scaffold has been shown to effect the way cells behave and integrate. In this study we examined the cellular response to scaffold architecture of novel electrospun scaffold for kidney tissue engineering. Fibre diameters of 1.10±0.16 µm and 4.49±0.47 µm were used with 3 distinct scaffold architectures. Traditional random fibres were spun onto a mandrel rotating at 250 rpm, aligned at 1800 rpm with novel cryogenic fibres spun onto a mandrel loaded with dry ice rotating at 250 rpm. Human kidney epithelial cells were grown for 1 and 2 weeks. Fibre morphology had no effect of cell viability in scaffolds with a large fibre diameter but significant differences were seen in smaller fibres. Fibre diameter had a significant effect in aligned and cryogenic scaffold. Imaging detailed the differences in cell attachment due to scaffold differences. These results show that architecture of the scaffold has a profound effect on kidney cells; whether that is effects of fibre diameter on the cell attachment and viability or the effect of fibre arrangement on the distribution of cells and their alignment with fibres. Results demonstrate that PCL scaffolds have the capability to maintain kidney cells life and should be investigated further as a potential scaffold in kidney tissue engineering.

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Section: Discussionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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“…Furthermore a successful differentiation of HMSCs into osteoblastic lineage and subsequent mineralization were observed on PCL nanofibers [76]. Wang et al cultured human embryonic stem cells on fibers made up of Tussah silk fibroin and having diameters of 400 and 800 nm and showed increased cellular viability on the 400 nm fibers [77]. In a study conducted by Yang et al, the rate of neural stem cells differentiation was shown higher on PLLA nanofibers than on microfibers [78].…”

Section: Electrospinningmentioning

confidence: 99%

Plasma surface functionalization of biodegradable electrospun scaffolds for tissue engineering applications

Ghobeira¹,

Morent²

2017

Biodegradable Polymers: Recent Developments and New Perspectives

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“…43,44 The proliferation and activity of the cells on the scaffold reduced with the increase of fiber diameter. 45 In this study, we found that ASCs and NSCs could proliferate on the scaffold well, which indicated that cells could survive on this scaffold (fiber diameter, 7.93±1.41 μm). The fibers with diameters between 400-500 nm were easier to adhere; however, the 3D space was not enough.…”

mentioning

confidence: 57%

In vitro study of neural stem cells and activated Schwann cells cocultured on electrospinning polycaprolactone scaffolds

Fan¹,

Zhou²,

Wang³

et al. 2017

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Background:To investigate the biocompatibility of electrospinning polycaprolactone (PCL) fiber scaffolds and coculture system, which consisted of neural stem cells (NSCs) and activated Schwann cells (ASCs). Materials and methods: ASCs were isolated from sciatic nerves, ligated for 7 days, in 4-week-old Wistar rats, and the NSCs were isolated from the hippocampus of E14.5 Wistar rat embryos. ASCs, NSCs and ASCs combined with NSCs were 3D cultured on the electrospinning PCL fiber scaffolds. Crystal violet staining was used to find the suitable density of ASCs for growth, and the proliferation of NSCs and ASCs were tested by Cell Counting Kit (CCK)-8 assay, and cell adhesion, differentiation of NSCs and myelin basic protein (MBP) expression of ASCs were observed by laser confocal microscopy. Distribution and morphology were assessed by scanning electron microscopy. Results: The average diameter of fibers in electrospinning PCL scaffolds was approximately 7.93±1.41 μm. ASCs could grow well at the density of 2×10 4 /cm 2 , and a certain number of cells extended along the longitudinal axis of fibers, and the shape of the cells was spindle, which was consistent with crystal violet staining results. The CCK-8 experiment showed ASCs could proliferate gradually on the PCL scaffold within 7 days, as well as NSCs, and NSCs differentiated into astrocytes, neurons and oligodendrocytes on the PCL scaffold; PCL scaffolds could improve the differentiation rate of neurons. After NSCs and ASCs were cocultured on electrospinning PCL scaffolds, ASCs could express MBP and NSCs could differentiate into neurons, which distributed around those ASCs expressing MBP. Conclusion: Electrospinning PCL fibrous scaffolds showed good biocompatibility, and the fibers had an inducing effect on the distribution of ASCs. NSCs and ASCs cultured on electrospinning PCL scaffolds could form 3D culture system, and NSCs could differentiate into neurons which distributed around the ASCs expressing MBP.

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The effects of electrospun TSF nanofiber diameter and alignment on neuronal differentiation of human embryonic stem cells

Cited by 99 publications

References 68 publications

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

Plasma surface functionalization of biodegradable electrospun scaffolds for tissue engineering applications

In vitro study of neural stem cells and activated Schwann cells cocultured on electrospinning polycaprolactone scaffolds

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