The effects of endogenous and exogenous nitric oxide on gut motility in zebrafish <i>Danio rerio</i> embryos and larvae

Holmberg, Anna; Olsson, Caroline; Holmgren, Susanne

doi:10.1242/jeb.02272

Cited by 73 publications

(88 citation statements)

References 45 publications

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“…Similar to MMC-like contractions previously described in teleosts (Brijs et al, 2014(Brijs et al, , 2017aHolmberg et al, 2007Holmberg et al, , 2006Holmberg et al, , 2003Holmberg et al, , 2004, this propagating complex closely resembles the different phases of mammalian MMCs in rats and mice (Bush et al, 2000;Deloose et al, 2012;Husebye, 1999). However, the relatively long period of quiescence and build-up of contractile activity prior to the cluster of contractions is also reminiscent of neurally mediated peristalsis (Costa and Furness, 1976;Hennig et al, 1999;.…”

Section: The Presence and Potential Osmoregulatory Role Of Mmcs In Rasupporting

confidence: 76%

Exposure to seawater increases intestinal motility in euryhaline rainbow trout (Oncorhynchus mykiss)

Brijs

Hennig

Gräns

et al. 2017

Journal of Experimental Biology

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Upon exposure to seawater, euryhaline teleosts need to imbibe and desalinate seawater to allow for intestinal ion and water absorption, as this is essential for maintaining osmotic homeostasis. Despite the potential benefits of increased mixing and transport of imbibed water for increasing the efficiency of absorptive processes, the effect of water salinity on intestinal motility in teleosts remains unexplored. By qualitatively and quantitatively describing in vivo intestinal motility of euryhaline rainbow trout (Oncorhynchus mykiss), this study demonstrates that, in freshwater, the most common motility pattern consisted of clusters of rhythmic, posteriorly propagating contractions that lasted ∼1-2 min followed by a period of quiescence lasting ∼4-5 min. This pattern closely resembles mammalian migrating motor complexes (MMCs). Following a transition to seawater, imbibed seawater resulted in a significant distension of the intestine and the frequency of MMCs increased twofold to threefold with a concomitant reduction in the periods of quiescence. The increased frequency of MMCs was also accompanied by ripple-type contractions occurring every 12-60 s. These findings demonstrate that intestinal contractile activity of euryhaline teleosts is dramatically increased upon exposure to seawater, which is likely part of the overall response for maintaining osmotic homeostasis as increased drinking and mechanical perturbation of fluids is necessary to optimise intestinal ion and water absorption. Finally, the temporal response of intestinal motility in rainbow trout transitioning from freshwater to seawater coincides with previously documented physiological modifications associated with osmoregulation and may provide further insight into the underlying reasons shaping the migration patterns of salmonids.

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Section: The Presence and Potential Osmoregulatory Role Of Mmcs In Rasupporting

confidence: 76%

Exposure to seawater increases intestinal motility in euryhaline rainbow trout (Oncorhynchus mykiss)

Brijs

Hennig

Gräns

et al. 2017

Journal of Experimental Biology

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“…In MA, we described an increase in Ach-mediated vasoconstriction indicating the presence of a coupled Ach-NOS-NO dilation. This evidence suggests that there is Ach-NOS-NO-relaxation coupling in MA in both species, such as that shown in Danio rerio in the same vascular area (Fritsche et al, 2000;Holmberg et al, 2006).…”

Section: Role Of L-name In the Ach-mediated Contractionsupporting

confidence: 64%

Role of NO in arterial vascular function of intertidal fish (Girella laevifrons) and marine fish (Isacia conceptionis)

Moraga

Urriola-Urriola

2016

Braz. J. Biol.

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Previous studies performed in intertidal fish (Girella laevifrons),as well as marine fish (Isacia conceptionis), showed that acetylcholine (ACh) produced contractions mediated by cyclooxygenases that were dependent on the area and potency of contraction in several arterial vessels. Given that the role of nitric oxide is poorly understood in fish, the objective of our study was to evaluate the role of nitric oxide in branchial afferent (ABA), branchial efferent (ABE), dorsal (DA) and mesenteric (MA) arterial vessels from both Girella laevifrons and Isacia conceptionis. We studied afferent and efferent branchial, dorsal and mesenteric arteries that were dissected from 6 juvenile specimens. Isometric tension studies were done using dose response curves (DRC) for Ach (10 -13 to 10 -3 M) and blockade with L-NAME (10 -5 M), and DRC for sodium nitroprusside (SNP, a donor of NO). L-NAME produced an attenuation of the contractile response in the dorsal, afferent and efferent branchial arteries and a potentiation of the contraction in the MA. SNP caused 70% dilation in the mesenteric artery and 40% in the dorsal artery. Our results suggest that Ach promotes precarious dilatation in MA mediated by NO; data that is supported by the use of sodium nitroprusside. In contrast, in the vessels DA, ABA and EBA our results support that the pathway Ach-NO-relaxation is absent in both species.Keywords: intertidal fish, marine fish, vascular reactivity, NO-pathway. Papel do NO na função vascular arterial de peixes entremarés (Girella laevifrons) e peixes marinhos (Isacia conceptionis) ResumoEstudos anteriores, realizados no peixe intertidal (Girellalaevifrons) no peixe marinho (Isacia conceptionis), mostram que a acetilcolina (Ach) provoca contrações mediadas por ciclooxigenases que eram dependentes da área e potencia da contração em vários vasos arteriais. Tendo em conta que o papel do óxido nítrico é mal compreendido em peixes, o objetivo do nosso estudo foi avaliar o papel do óxido nítrico em vasos arteriais de ambos os peixes Girella laevifrons e Isacia conceptionis. Nós estudamos os vasos aferente, branquial (ABA), eferente branquial (ABE), dorsal (DA) e mesentérica (MA), que foram dissecadas de seis espécimes juvenis. Estudos de tensão isométrica foram realizados utilizando as curvas de dose-resposta (DRC) para Ach (10 -13 a 10 -3M) e bloqueio com L-NAME (10 -5 M), e na DRC para o nitroprussiato de sódio (SNP, doador do NO). L-NAME produziu uma atenuação da resposta contrátil nas artérias dorsais, aferentes e eferentes branquial e uma potenciação da contração no MA. SNP causaram 70% da dilatação da artéria mesentérica e 40% na artéria dorsal. Nossos resultados sugerem que Ach promove dilatação precária em MA mediada por NO; dados que é suportada pela utlilização de nitroprussiato de sódio. Em contraste, nos vasos de DA, ABA e EBA nossos resultados suportam que a via de Ach-NO-relaxamento está ausente em ambas as espécies.Palavras-chave: peixe entremarés, peixe marinho, reatividade vascular, NO-pathway.

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“…Inhibitory and excitatory neurons of the enteric nervous system are associated with the gut by 2 dpf, and spontaneous, sporadic contractions (demonstrating smooth muscle contractility) are observed by 3 dpf (Holmberg et al, 2004). Nitric oxide synthase immunoreactivity in the enteric neurons also increases between 3 and 4 dpf (Holmberg et al, 2006).…”

Section: Introductionmentioning

confidence: 98%

Spatiotemporal expression of smooth muscle markers in developing zebrafish gut

Georgijevic

Subramanian

Rollins

et al. 2007

Developmental Dynamics

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Smooth muscle is important for the contractility and elasticity of visceral organs. The zebrafish is an excellent model for understanding embryonic development, yet due to a lack of appropriate markers, visceral smooth muscle development remains poorly characterized. Here, we develop markers and trace the development of gut and swim bladder smooth muscle in embryonic and juvenile fish. The first smooth muscle marker we detect in the vicinity of the gut is the myoblast marker nonmuscle myosin heavy chain-b at 50 hours postfertilization (hpf), followed by the early smooth muscle markers SM22␣-b, and ␣-smooth muscle actin at 56 and 60 hpf, respectively. Markers of more differentiated smooth muscle, smoothelin-b and cpi-17, appear by 3 days postfertilization (dpf). Tropomyosin, a relatively late marker, is first expressed at 4 dpf. We find that smooth muscle marker expression in the swim bladder follows the same sequence of marker expression as the gut, but markers have a temporal delay reflecting the later formation of swim bladder smooth muscle. Developmental Dynamics 236:1623-1632, 2007.

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The effects of endogenous and exogenous nitric oxide on gut motility in zebrafish Danio rerio embryos and larvae

Cited by 73 publications

References 45 publications

Exposure to seawater increases intestinal motility in euryhaline rainbow trout (Oncorhynchus mykiss)

Exposure to seawater increases intestinal motility in euryhaline rainbow trout (Oncorhynchus mykiss)

Role of NO in arterial vascular function of intertidal fish (Girella laevifrons) and marine fish (Isacia conceptionis)

Spatiotemporal expression of smooth muscle markers in developing zebrafish gut

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