1972
DOI: 10.1113/expphysiol.1972.sp002147
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The Effects of Endogenous and Added Insulin on the Time‐course of Glucose Uptake by the Isolated Perfused Rat Heart

Abstract: A new method for measurement at short intervals of uptake or production of metabolites by a perfused heart has been applied to the study of the time-course of glucose utilization. In the presence of high concentrations of insulin (100 mU/ml.) glucose utilization was constant from the beginning of perfusion at all concentrations of glucose. In the absence of added insulin, when the perfusate glucose concentration was greater than 1 mm, glucose utilization decreased by more than 20% to reach a constant rate afte… Show more

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Cited by 10 publications
(12 citation statements)
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“…Hearts were perfused by the method of Fisher and O'Brien [25], except that the blood was washed from the heart with warmed oxygenated perfusate [14]. Hearts were perfused at a pressure of 40mm Hg; the perfusate temperature was 37 °C.…”
Section: Perfusion Methodsmentioning
confidence: 99%
“…Hearts were perfused by the method of Fisher and O'Brien [25], except that the blood was washed from the heart with warmed oxygenated perfusate [14]. Hearts were perfused at a pressure of 40mm Hg; the perfusate temperature was 37 °C.…”
Section: Perfusion Methodsmentioning
confidence: 99%
“…We refer to this fluid as 'secretion'. A modified Starling resistance, H (Fisher & O'Brien, 1972), is connected to the outflow cannula to maintain a distension pressure of 30 cm water so as to expose uniformly the whole mucosal surface to the perfusate and gas. The outflow from the resistance passes to a second set of tared collecting tubes at K. The sets of tubes, J and K, are held in a time operated fraction collector.…”
Section: Kinetic Approach To Absorption 213mentioning
confidence: 99%
“…Glucose was estimated on a Technicon AutoAnalyzer by Fisher & O'Brien's (1972) modification of the glucose oxidase method of Hill & Cowart (1966), which uses gum guaiacum extract as chromogen. The S.D.…”
Section: Kinetic Approach To Absorption 213mentioning
confidence: 99%
“…But where there is recirculation through the lumen the uptake so calculated is the algebraic sum of the mucosal uptake and the change in solute content of the recirculating fluid [Fisher and O'Brien, 1972]. To calculate the second term an exponential is fitted to the mean concentration (5) in successive collections, giving j=p+qe-kt.…”
Section: Calculations Mucosal Uptakefrom the Lumenmentioning
confidence: 99%