THE EFFECTS OF ETHANOL EXTRACTS OF Centella asiatica LEAF ON SERIAL SERUM BRAIN DERIVED NEUROTROPHIN FACTOR (BDNF) CONCENTRATION OF RATS (SPRAGUE DAWLEY) FOLLOWING CHRONIC STRESS

Sari, Dwi Cahyani Ratna; Rochmah, Mawaddah Ar

doi:10.18502/kls.v2i1.136

Cited by 6 publications

(7 citation statements)

References 31 publications

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“…The plasma concentration of TNF- α as well as other inflammatory cytokines increases under stressful condition such as restraint stress [ 35 ]. Therefore, it would be feasible to use serial plasma TNF- α for evaluating stress response in rodents to chronic stress paradigms, such as what has been shown for serum BDNF [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Centella asiaticaPrevents Increase of Hippocampal Tumor Necrosis Factor-αIndependently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of Chronic Stress

Rochmah

Harini

Septyaningtrias

et al. 2019

BioMed Research International

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Centella asiaticaameliorates memory impairment and induces expression of hippocampal brain-derived neurotropic factor (BDNF) in chronically stressed rats. The relationship between the anti-inflammatory effect ofCentella asiaticaon hippocampal BDNF and the involvement of sirtuin-1, a BDNF expression regulator, in neuroprotective mechanisms ofCentella asiaticawarrants an investigation. We investigated the effect ofCentella asiaticaethanolic extracts (CA) on TNF-α, IL-10, and SIRT1 levels and whether these predicted BDNF expression in rat hippocampus after chronic stress. For the experiments, thirty male rats (Sprague Dawley) were divided into six groups: nonstressed-control, stressed-control, nonstressed +CA 300mg/kg/d, stressed +CA 150 mg/kg/d, stressed +CA 300 mg/kg/d, and stressed +CA 600 mg/kg/d. On day 28, rats were sacrificed and hippocampus was dissected out. Hippocampal TNF-α, IL-10, SIRT1, and BDNF were measured by enzyme-linked immunosorbent assay. Hippocampal TNF-αlevel was significantly higher in the stressed-control compared to nonstressed-control groups. Across all stress conditions, rats receiving the highest dose of CA had the lowest mean TNF-αand highest mean BDNF. There were no significant differences in IL-10 and SIRT1 levels between groups. Hippocampal TNF-αdid not predict hippocampal BDNF in a regression analysis. In conclusion, lower TNF-αand higher BDNF in the hippocampus support the hypothesis that these factors independently contribute toCentella asiatica’s neuroprotective effect in chronically stressed rats.

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Section: Discussionmentioning

confidence: 99%

Centella asiaticaPrevents Increase of Hippocampal Tumor Necrosis Factor-αIndependently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of Chronic Stress

Rochmah

Harini

Septyaningtrias

et al. 2019

BioMed Research International

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“…The two main components in pegagan are triterpenoids and flavonoids. Triterpenoids consists of active components, namely asiatic acid and asiaticoside (Sari and Rochmah, 2015).…”

Section: Resultsmentioning

confidence: 99%

“…There are two main components in pegagan, namely triterpenoids and flavonoids. Both components function as antioxidants and are neuroprotective (Sari and Rochmah, 2015, Orhan, 2012, Vauzour et al, 2008. Pegagan has long been used in Ayurveda medicine and traditional Chinese medicine to improve memory function (Lokanathan et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Pegagan (Centella Asiatica (L.) Urban) Ethanol Extracts on Hippocampal PSD-95 Protein Expression in Male Wistar Rats

et al. 2018

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One effort to overcome the decline in memory function is through herbal medicine. Pegagan (Centella asiatica (L.) Urban) contain the active components of triterpenoid and flavonoids, has been known to be able to improve memory function. Synaptic plasticity is the basis of memory formation which is strongly influenced by synapse proteins such as PSD-95. Loss of PSD-95 protein can cause memory function decline. This study aims to determine the effect of 70% ethanol extract of pegagan toward PSD-95 protein expression on hippocampus of male Wistar rat. Eighteen male Wistar rats were randomly divided into 3 groups, 6 rats/group: group (1) given ethanol extract of pegagan with dose 300 mg / kgBW / day (CA300), (2) given ethanol extract of pegagan with dose 600 mg / kgBW / day (CA600), and (3) control group (K), given daily aquadest. All three groups were treated for 28 consecutive days. At the end of the treatment period, rats were decapitated and the hippocampus was isolated from the brain. Analysis of protein was done by immunohistochemical method. Statistical analysis was performed by One Way ANOVA parametric test followed by Post-Hoc Bonferroni. The results showed that there was no significant difference between group K and group CA300 (P = 0.123), whereas there were significant differences between CA600 group and K group and CA300 group (P = 0.000). From this research, it can be concluded that 70% ethanol extract of pegagan with dose 600mg / kgBW / day can increase expression of PSD-95 protein on hippocampus of male Wistar rat.

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“…in rats following chronic stress affects the concentration of serum and hippocampal brain-derived neurotrophic factor (BDNF), which is an endogenous neuroprotective agent in the brain. [4][5][6] The brain is the central organ for stress adaptation and is also a target of stress. 6 Chronic stress may result in abnormal changes in brain plasticity, including dendritic retraction, neuronal toxicity and suppression of neurogenesis, as well as axospinous synaptic plasticity.…”

Section: Abstrakmentioning

confidence: 99%

Ethanolic extract of the Centella asiatica (L.) Urb. leaf decreases cerebellar brain-derived neurotrophic factor (BDNF) levels in rats after chronic stress

Sari¹,

Juananda²,

Ar-Rochmah³

et al. 2018

JmedScie

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Chronic stress produces glucocorticoid-induced neurotoxicity that may lead to alterations of the brain-derived neurotrophic factor (BDNF) concentration in the brain. Cerebellum is known to be severely affected by glucocorticoids-associated oxidative damage. Centella asiatica (L.) Urb. may protect neurons from oxidative damage. This study aimed to investigate the effect of ethanolic extract of C. asiatica (L.) Urb. leaf on the rat cerebellar BDNF levels following stress. Twenty young-adult male Sprague Dawley rats were randomly assigned into four experimental groups. The stress control group received aquadest, and the other groups were treated with different doses of the C. asiatica (L.) Urb. extract i.e 150 (CeA150), 300 (CeA300) and 600 (CeA600) mg/kg body weight/ day orally, respectively and followed by chronic footshock stress for 28 days. Upon completion of the experimental period, all animals were sacrificed and the cerebellar was isolated. The BDNF levels from the cerebellar tissue lysate was measured using ELISA. The mean BDNF levels of the cerebellar tissue in the stress control, CeA150, CeA300 and CeA600 groups were 1217.10±301.40; 771.46±241.45; 757.05±268.29; and 627.00±246.02 pg/mL, respectively. Post-hoc analysis showed a significant difference between the control and treatment groups (p< 0.05). In conclusion, the ethanolic extracts of the C. asiatica (L.) Urb. leaf decrease the cerebellar BDNF levels in rats after chronic stress.

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THE EFFECTS OF ETHANOL EXTRACTS OF Centella asiatica LEAF ON SERIAL SERUM BRAIN DERIVED NEUROTROPHIN FACTOR (BDNF) CONCENTRATION OF RATS (SPRAGUE DAWLEY) FOLLOWING CHRONIC STRESS

Cited by 6 publications

References 31 publications

Centella asiaticaPrevents Increase of Hippocampal Tumor Necrosis Factor-αIndependently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of Chronic Stress

Centella asiaticaPrevents Increase of Hippocampal Tumor Necrosis Factor-αIndependently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of Chronic Stress

The Effect of Pegagan (Centella Asiatica (L.) Urban) Ethanol Extracts on Hippocampal PSD-95 Protein Expression in Male Wistar Rats

Ethanolic extract of the Centella asiatica (L.) Urb. leaf decreases cerebellar brain-derived neurotrophic factor (BDNF) levels in rats after chronic stress

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