The Effects of Extracellular Matrix on Cell Attachment, Proliferation and Migration in a Human Lens Epithelial Cell Line

Oharazawa, Hideaki; Ibaraki, Nobuhiro; Lin, Lishan; Reddy, Venkat N.

doi:10.1006/exer.1999.0723

Cited by 66 publications

(35 citation statements)

References 28 publications

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“…In the lens, the ECM is represented by the lens capsule, which is primarily composed of collagen IV, laminin, and fibronectin (Cammarata et al, 1986). As might be expected, lens epithelial cells in culture attach and spread well on these substrates, providing direct evidence that they recognize and bind these components ( Oharazawa et al, 1999;Negash et al, 2002) (Fig. 2).…”

Section: Lens Extracellular Matrixmentioning

confidence: 86%

Regulation of cell adhesion and migration in lens development

Zelenka¹

2004

Int. J. Dev. Biol.

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Cell movements during lens development and differentiation involve dynamic regulation of cell-matrix and cell-cell adhesion. How these processes are regulated depends on the particular array of matrix components and adhesion proteins that are expressed, as well as the signaling pathways that affect them. This review examines what is known about adhesion proteins and their regulation in the lens in light of recent findings about the mechanism of cell migration. The characteristic shape and organization of the lens depends on highly regulated cell movements during development and differentiation. Epithelial cells at the equator migrate posteriorly, bringing them into contact with factors in the vitreous humor and initiating differentiation. Elongation of the differentiating fiber cells is coupled with directed migration, posteriorly along the capsule and anteriorly along the fiber cell-epithelial interface, to generate a symmetrically organized fiber cell mass with aligned suture planes. To make these movements, cells systematically create and dissolve cell-cell and cell-matrix adhesions, form connections between these adhesions and the cytoskeleton, and generate contractile force. Since errors in cell migration may lead to aberrant lens shape or misplacement of the lens sutures, precise regulation of each step is essential for the optical quality of the lens. Recent advances in cellular developmental biology have begun to shed light on the molecular mechanisms underlying cell movements and the changes in adhesion that make them possible. This review will summarize those findings and relate them to relevant studies of the lens to provide an outline of the cellular events that lead to lens morphogenesis.

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Section: Lens Extracellular Matrixmentioning

confidence: 86%

Regulation of cell adhesion and migration in lens development

Zelenka¹

2004

Int. J. Dev. Biol.

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“…Although in our study the mean superficial epithelial cell density was lower in buphthalmic eyes, the difference was not statistically significant. Proliferative activity of different cells can be affected by the action of mechanical factors, [30][31][32] basement membrane modifications, [33][34][35] and effects of growth factors. 36,37 Different growth factors have been identified that can modify corneal epithelial cell mitotic activity.…”

Section: Discussionmentioning

confidence: 99%

Corneal Changes Assessed Using Confocal Microscopy in Patients With Unilateral Buphthalmos

Mahelková

Filouš

Odehnal

et al. 2013

Invest. Ophthalmol. Vis. Sci.

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Citation: Mahelková G, Filouš A, Odehnal M, Cendelín J. Corneal changes assessed using confocal microscopy in patients with unilateral buphthalmos. Invest Ophthalmol Vis Sci. 2013;54:4048-4053. DOI:10. 1167/iovs.12-11165 PURPOSE. To compare corneal structures in buphthalmic eyes and healthy eyes in patients with unilateral congenital glaucoma using a corneal confocal microscope.METHODS. Ten patients with unilateral buphthalmos (mean 6 SD age, 14.85 6 5.12 years) were examined using corneal confocal microscopy. The cell density and cell area of endothelial cells and superficial and basal epithelial cells and the number of keratocytes were evaluated.RESULTS. There was no significant difference between the cell density of superficial epithelial cells in buphthalmic eyes relative to healthy eyes (P ¼ 0.1944). The cell density of basal epithelial cells was significantly higher (P ¼ 0.0234) and the cell area was significantly smaller (P ¼ 0.0181) in buphthalmic eyes relative to healthy eyes. There was no difference between the number of keratocytes in buphthalmic eyes and healthy eyes in the anterior stroma (P ¼ 0.273) or in the posterior stroma (P ¼ 0.0799). The cell density of endothelial cells was significantly lower and the cell area was significantly larger in buphthalmic eyes relative to healthy eyes (P ¼ 0.0009). CONCLUSIONS.We demonstrated a lower cell density of endothelial cells in buphthalmic eyes. We found no differences in keratocyte density between the buphthalmic eyes and healthy eyes. The cell density of basal epithelial cells was higher in buphthalmic eyes. These differences could be due to buphthalmos or due to the previous surgical and medical therapies. Monitoring of these changes could help to contribute to accurate assessments regarding future ocular surgical procedures.

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“…The fibronectin and laminin, which are present in the serum [31], promote LEC migration [32,33]. Differences in the affinity of these extracellular matrixes for IOLs made of PMMA, silicone and acrylic have been reported [34,35].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Lens Epithelial Cell Migration by an Acrylic Intraocular Lens in vitro

Kurosaka

Obasawa²,

Kurosaka³

et al. 2002

Ophthalmic Res

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We developed a new in vitro system to evaluate the effect of intraocular lenses (IOLs) on the migration of lens epithelial cells (LECs) and determined how acrylic and other IOLs influence LEC migration using this model. In an in vitro system, porcine LECs were cultured in a cell culture chamber insert, containing a collagen membrane, for 10 days with no IOL or with various types of IOLs. Migration of LECs beneath each IOL optic was observed with an inverted-phase microscope. The cell-free areas under the IOL optic, where the LECs had not migrated, were measured. Without IOL, LECs completely covered the collagen membrane within 5 days after plating (5.0 ± 0.0 days). Complete coverage was slowed by a silicone IOL (6.7 ± 1.2 days, p = 0.0305). LECs cultured with acrylic or with round- or sharp-edged polymethylmethacrylate (PMMA) IOLs did not completely cover the area. Ten days after initiating cultures, the cell-free areas under IOLs with sharp edges (acrylic, 41.1 ± 8.0%; sharp-edged PMMA, 60.9 ± 39.0%) were significantly larger than under IOLs with round edges (silicone, 0.0 ± 0.0%; round-edged PMMA, 1.5 ± 1.2%). A sharp edge may act as a barrier to LEC migration. Moreover, LEC migration under the acrylic IOL slowed after the LECs had crossed the barrier of the optic edge, perhaps due to acrylic adhesive properties. Only a few LECs reached the collagen membrane beneath the central 3 mm of the acrylic IOL. This new in vitro model was useful in evaluating the effect of various IOLs on LEC migration. Acrylic IOLs inhibited LEC migration by not only a sharp edge but also other factors, such as adhesive properties.

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The Effects of Extracellular Matrix on Cell Attachment, Proliferation and Migration in a Human Lens Epithelial Cell Line

Cited by 66 publications

References 28 publications

Regulation of cell adhesion and migration in lens development

Regulation of cell adhesion and migration in lens development

Corneal Changes Assessed Using Confocal Microscopy in Patients With Unilateral Buphthalmos

Inhibition of Lens Epithelial Cell Migration by an Acrylic Intraocular Lens in vitro

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