The effects of fibrin–icariin nanoparticle loaded in poly (lactic-co-glycolic) acid scaffold as a localized delivery system on chondrogenesis of human adipose-derived stem cells

Gorji, Mona; Ghasemi, Nazem; Setayeshmehr, Mohsen; Zargar, A.; Kazemi, Mohammad; Soleimani, Mitra; Hashemibeni, Batool

doi:10.4103/abr.abr_143_19

Cited by 10 publications

(1 citation statement)

References 47 publications

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“…Hybrid fibrous scaffolds consist of both advantages of ECM derivatives and synthetic polymers can mimic natural microenvironment to differentiate stem cells, and can be considered a strong vehicle for TE applications [14]. Fibrin has been used as scaffolds for a variety of TE applications such as using in liver, skin, bone, cartilage [15]and cardiovascular applications [16]. However due to its poor mechanical properties its use as a perfect scaffold has been restricted [4].…”

Section: Introductionmentioning

confidence: 99%

Cartilage Particles can Promote Chondrogenesis of Adipose-Derived Stromal Cells on Poly(ε-Caprolactone)/Fibrin Hybrid Constructs Prepared via Sandwich Model

Ghosouri

Setayeshmehr

Taheri‐Kafrani

et al. 2020

JBBBE

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Electrospun fibers have demonstrated a remarkable potential as a framework structure in the fabrication of cartilage tissue engineering (CTE) scaffolds. Various extracellular matrices have been incorporated into electrospun scaffolds to mimic and simulate the extracellular environment. The objective of this study was to fabricate hybrid constructs using composite electrospun scaffolds based on poly (ε-caprolactone) (PCL) and cartilage-derived matrix (CDM) and fibrin hydrogel to improve the viability and differentiation of human adipose-derived stromal cells (ADSCs) for CTE applications.Initially, PCL and PCL-CDM electrospun mats were fabricated. Fibrin/ ADSCs hydrogel were seeded on PCL- CDM mats and arranged layer-by-layer using sandwich technique. This method has been employed to increase cell seeding and infiltration efficiency through the entire mass of the scaffold. Real-time reverse-transcription polymerase chain reaction (RT- PCR), were performed to examine the expression of collagen types II and X, SOX9 and aggrecan. The production of glycosaminoglycan (GAG) was also tested in vitro by Toluidine blue stain and biochemical assay in the cultured scaffolds.The findings demonstrated that incorporation of CDM in PCL fibers results in improved cell viability. Hematoxylin and eosin staining showed that the sandwich method resulted in homogenous cell seeding within the scaffold. Overall, the RT- PCR, biochemical and histological results, showed that incorporation of the CDM into PCL/fibrin sandwich scaffolds stimulated ADSCs chondrogenesis and produced the products which increased expression of chondrogenic genes. It also, enhanced GAG synthesis compared to PCL/fibrin scaffolds.These findings suggest PCL-CDM/fibrin can be considered as an appropriate hybrid scaffold for CTE applications.

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Section: Introductionmentioning

confidence: 99%

Cartilage Particles can Promote Chondrogenesis of Adipose-Derived Stromal Cells on Poly(ε-Caprolactone)/Fibrin Hybrid Constructs Prepared via Sandwich Model

Ghosouri

Setayeshmehr

Taheri‐Kafrani

et al. 2020

JBBBE

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A modular approach to 3D-printed bilayer composite scaffolds for osteochondral tissue engineering

Maherani,

Eslami,

Poursamar

et al. 2024

J Mater Sci: Mater Med

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Prolonged osteochondral tissue engineering damage can result in osteoarthritis and decreased quality of life. Multiphasic scaffolds, where different layers model different microenvironments, are a promising treatment approach, yet stable joining between layers during fabrication remains challenging. To overcome this problem, in this study, a bilayer scaffold for osteochondral tissue regeneration was fabricated using 3D printing technology which containing a layer of PCL/hydroxyapatite (HA) nanoparticles and another layer of PCL/gelatin with various concentrations of fibrin (10, 20 and 30 wt.%). These printed scaffolds were evaluated with SEM (Scanning Electron Microscopy), FTIR (Fourier Transform Infrared Spectroscopy) and mechanical properties. The results showed that the porous scaffolds fabricated with pore size of 210–255 µm. Following, the ductility increased with the further addition of fibrin in bilayer composites which showed these composites scaffolds are suitable for the cartilage part of osteochondral. Also, the contact angle results demonstrated the incorporation of fibrin in bilayer scaffolds based on PCL matrix, can lead to a decrease in contact angle and result in the improvement of hydrophilicity that confirmed by increasing the degradation rate of scaffolds containing further fibrin percentage. The bioactivity study of bilayer scaffolds indicated that both fibrin and hydroxyapatite can significantly improve the cell attachment on fabricated scaffolds. The MTT assay, DAPI and Alizarin red tests of bilayer composite scaffolds showed that samples containing 30% fibrin have the more biocompatibility than that of samples with 10 and 20% fibrin which indicated the potential of this bilayer scaffold for osteochondral tissue regeneration. Graphical Abstract

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Advances in nanoenabled 3D matrices for cartilage repair

2022

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The effects of fibrin–icariin nanoparticle loaded in poly (lactic-co-glycolic) acid scaffold as a localized delivery system on chondrogenesis of human adipose-derived stem cells

Cited by 10 publications

References 47 publications

Cartilage Particles can Promote Chondrogenesis of Adipose-Derived Stromal Cells on Poly(ε-Caprolactone)/Fibrin Hybrid Constructs Prepared via Sandwich Model

Cartilage Particles can Promote Chondrogenesis of Adipose-Derived Stromal Cells on Poly(ε-Caprolactone)/Fibrin Hybrid Constructs Prepared via Sandwich Model

A modular approach to 3D-printed bilayer composite scaffolds for osteochondral tissue engineering

Advances in nanoenabled 3D matrices for cartilage repair

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