The effects of fibrolytic enzymes, cellulolytic fungi and bacteria on the fermentation characteristics, structural carbohydrates degradation, and enzymatic conversion yields of Pennisetum sinese silage

Li, Junfeng; Yuan, Xianjun; Dong, Zhihao; Mugabe, Wazha; Shao, Tao

doi:10.1016/j.biortech.2018.05.059

Cited by 69 publications

(55 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An exponential decay model was used to fit the WSC and monosaccharide (glucose, fructose, and xylose) data using IBM Statistical Packages for the Social Sciences (IBM SPSS 20.0 for Windows) to describe the sugar change characteristics with the following equation:where y (g/kg DM) is the residue at any time x (day), y 0 (g/kg DM) is the total residual fraction after 60 days of ensiling, a (g/kg DM) is the consumable fraction, b (day −1 ) is the fractional consumption rate of a and x is the ensilage time (day) [4].…”

Section: Methodsmentioning

confidence: 99%

“…Ensiling is a bioprocessing method for the anaerobic preservation and pretreatment of lignocellulosic biomass to produce feed and biofuel [3, 4]. However, alfalfa contains low contents of dry matter (DM) and water-soluble carbohydrates (WSC), especially in the rainy season, and has a high buffer capacity that often causes increased butyric acid and substantial DM loss [5].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enhancement of lignocellulosic degradation in high-moisture alfalfa via anaerobic bioprocess of engineered Lactococcus lactis with the function of secreting cellulase

Liu

Zhao

et al. 2019

Biotechnol Biofuels

Self Cite

View full text Add to dashboard Cite

Background Butyric fermentation and a substantial loss of dry matter (DM) often occur in alfalfa silage during the rainy season, which is not conducive to subsequent biofuel production. Currently, there have been negative effects on the combination of cellulases and lactic acid bacteria (LAB) on processing high-moisture alfalfa silage; however, transgenically engineered LAB strains that secrete cellulase have been proposed as an alternative approach to avoid the above problem. The objective of the present study was to construct engineered Lactococcus lactis strains with high-efficiency secretory-expressing cellulase genes from Trichoderma reesei and to investigate the effects of the combination of transgenically engineered L. lactis strains HT1 / pMG36e- usp 45- bgl 1, HT1 / pMG36e- usp 45- cbh 2, and HT1 / pMG36e- usp 45- egl 3 (HT2) on fermentation quality, structural carbohydrate degradability and nonstructural carbohydrate fermentation kinetics of high-moisture alfalfa silage treated without additive as a negative control (Control), or/and with cellulase (EN), wild-type L. lactis subsp. lactis MG1363 (HT1) and the combination of HT1 and EN (HT1 + EN) as positive additive controls. Results Engineered L. lactis strains were successfully constructed and efficiently secreted endoglucanase (1118 mU/mL), cellobiohydrolase (222 mU/mL), and β-glucosidase (131 mU/mL) and had high filter paper activity (236 mU/mL). Ensiling experiments verified that HT2 obtained the highest fermentation quality score (83.6) and most efficiently processed high-moisture alfalfa silage, demonstrated by a low pH (4.49) and ammonia-N content (106 g/kg nitrogen) and a high lactic acid content (67.1 g/kg DM) and without butyric acid. Change curves of structural carbohydrates revealed that HT2 degraded more lignocelluloses, demonstrated by the lowest contents of neutral detergent fibre, acid detergent fibre, cellulose and hemicellulose after ensiling for 60 days. Kinetic analysis showed that the most residual water-soluble carbohydrates, glucose, fructose and xylose generated by lignocellulose degradation were produced by HT2, followed by HT1 + EN. The HT2-treated silages had the highest DM recovery, had the fewest Clostridia spores, emitted a fragrance and were not sticky. Conclusion HT2 improved the conversion of lignocellulose to sugars and processed high-moisture alfalfa silage efficiently. This is a novel strategy that can be used to enhance lignocellulosic degradation in high-moisture alfalfa via a bioprocess with transgenically engineered L. lactis ...

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhancement of lignocellulosic degradation in high-moisture alfalfa via anaerobic bioprocess of engineered Lactococcus lactis with the function of secreting cellulase

Liu

Zhao

et al. 2019

Biotechnol Biofuels

Self Cite

View full text Add to dashboard Cite

show abstract

“…The untreated macroalgae showed compact and intact surface with salt crystals while after SSF and HE the surface of macroalgae presented high roughness and many holes. It is known that NSP enzymes action causes undulating surfaces and large quantity of hollow regions (Li et al, 2018). The branching hyphal morphology of the fungus allowed it to colonize all substrate and to release carbohydrase enzymes, which strongly degraded macroalgae polysaccharides during EH.…”

Section: Sem Analysismentioning

confidence: 99%

Sequential bioprocessing of Ulva rigida to produce lignocellulolytic enzymes and to improve its nutritional value as aquaculture feed

Fernandes

Salgado

Martins

et al. 2019

Bioresource Technology

View full text Add to dashboard Cite

A B S T R A C TThe macroalgae aquaculture industry has grown up in the last years, and new applications for macroalgae should be considered. In this work, sequential biological treatments as solid-state fermentation (SSF) by Aspergillus ibericus and enzymatic hydrolysis (EH) were applied to washed and unwashed Ulva rigida. SSF of unwashed macroalgae showed higher xylanase (359.8 U/g), cellulase (73.07 U/g) and β-glucosidase (14.9 U/g) activities per dry mass of macroalgae. After SSF, two strategies to carry out EH were assayed. The best process was SSF followed by EH by simply adding a buffer. The non-starch polysaccharides content was reduced by 93.2%, achieving a glucan conversion of 98%. In addition, the antioxidant activity was improved 2.8-fold and the protein concentration of macroalgae extracts increased from 16.9% to 29.8% (w/w). These biological treatments allowed to increase macroalgae value as feedstuff with potential for use in aquafeeds. 2015). Even thought, macroalgae are usually dried to be preserved, SSF

show abstract

“…We hypothesized that LAB from animals not only could improve the silage quality, but also be more likely to have a good environmental adaptability in animals, subsequently improving animal performance. Previous research showed that Enterococcus faecium isolated from yak rumen could be exploited for reducing the lignocellulosic biomass of grass silage (Li et al, 2018c). However, until now, little information is available about the effects of LAB derived from dairy cow on silage quality.…”

Section: Introductionmentioning

confidence: 99%

Effects of Lactic Acid Bacteria Isolated From Rumen Fluid and Feces of Dairy Cows on Fermentation Quality, Microbial Community, and in vitro Digestibility of Alfalfa Silage

Guo

Yao

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

The objective of this study was to select lactic acid bacteria (LAB) isolated from the rumen fluid and feces of dairy cows, and evaluate their effects on silage quality of alfalfa after 30 or 60 days of ensiling. One hundred and four LAB strains were isolated from rumen fluid and feces of six dairy cows, of which four strains (Lactobacillus plantarum F1, L. plantarum F50, Lactobacillus salivarius L100, and Lactobacillus fermentum L120) and one commercial inoculant (GFG) isolated from forage were employed for further study. The silages treated with F1 had the lowest (P < 0.05) pH value and the highest (P < 0.05) lactic acid (LA) content in all treatments. Besides, higher (P < 0.05) in vitro digestibility was also observed in F1-treated silage after 60 days of ensiling. The microbial analysis showed that the Lactobacillus abundance in the F1-treated silages increased to 60.32%, higher than other treatments (5.12-47.64%). Our research indicated that strain F1 could be an alternative silage inoculant, and dairy cows could be a source for obtaining excellent LAB for ensiling.

show abstract

The effects of fibrolytic enzymes, cellulolytic fungi and bacteria on the fermentation characteristics, structural carbohydrates degradation, and enzymatic conversion yields of Pennisetum sinese silage

Cited by 69 publications

References 35 publications

Enhancement of lignocellulosic degradation in high-moisture alfalfa via anaerobic bioprocess of engineered Lactococcus lactis with the function of secreting cellulase

Enhancement of lignocellulosic degradation in high-moisture alfalfa via anaerobic bioprocess of engineered Lactococcus lactis with the function of secreting cellulase

Sequential bioprocessing of Ulva rigida to produce lignocellulolytic enzymes and to improve its nutritional value as aquaculture feed

Effects of Lactic Acid Bacteria Isolated From Rumen Fluid and Feces of Dairy Cows on Fermentation Quality, Microbial Community, and in vitro Digestibility of Alfalfa Silage

Contact Info

Product

Resources

About