The effects of genetics and age on expression of MHC class II and CD4 antigens on rat cardiac interstitial dendritic cells

Darden, Alix G.; Forbes, R. D. C.; Darden, Paul M.; Guttmann, Ronald D.

doi:10.1016/0008-8749(90)90324-k

Cited by 14 publications

(6 citation statements)

References 16 publications

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“…In addition to OX6 antibody, previous studies reported that W3/25 antibody also detected cardiac dendritic cells [3,20]. In the present study, W3/25-positive dendritic cells were more numerous than OX6-positive dendritic cells in normal heart, but the latter cells increased more sharply and were always more numerous than the former after immunization.…”

Section: Characteristics Of Cardiac Dendritic Cellsmentioning

confidence: 33%

A histological study on experimental autoimmune myocarditis with special reference to initiation of the disease and cardiac dendritic cells

Suzuki

1995

Vichows Archiv A Pathol Anat

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The precise mechanism of myosin-induced autoimmune myocarditis is unknown. The purpose of the present study was to define the immunohistological and ultrastructural characteristics of the infiltrating cells, especially in the initial phase of the myocarditis. It was demonstrated that OX6-positive dendritic cells first infiltrated the cardiocytes on day 13 after immunization. After day 17, OX6-positive cells, which possessed elongated irregular-shaped processes on the cell surface but contained few phago-lysosomes in the cytoplasm, were located at the margin of an inflammatory field and inserted their processes into the sarcoplasm of cardiocytes. The central portion of the inflammatory field was occupied by ED1-positive inflammatory macrophages, which were rich in phagosomes and which were in contact with degenerating cardiocytes. No evidence was obtained which suggested that lymphocytes directly injured the cardiocytes. These results demonstrated ultrastructural evidence that the type of infiltrating cell that first injures cardiocytes is the cardiac dendritic cell. Inflammatory macrophages thereafter serve as scavengers of degenerating cardiocytes.

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Section: Characteristics Of Cardiac Dendritic Cellsmentioning

confidence: 33%

A histological study on experimental autoimmune myocarditis with special reference to initiation of the disease and cardiac dendritic cells

Suzuki

1995

Vichows Archiv A Pathol Anat

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“…Immunostaining for ED1 (monocytes/macrophages), ED2 (macrophages/Kupffer cells) and OX6, identifies two distinct phenotypes of hepatic DC: (i) ED1 + ED2 – OX6 + and (ii) ED1 – ED2 – OX6 + 10 . Interestingly, rat hepatic MHC class II + DC appear to express variable levels of CD4, 3 , 12 which is common in DC in other peripheral tissues of this species 3 , 13 , 14 . Mouse CD11c + 4 and CD205 + cells 15 are mainly located periportally, as reported for MHC II + cells.…”

Section: Characterization Of Hepatic Dendritic Cells In Situmentioning

confidence: 91%

Immunobiology of liver dendritic cells

et al. 2002

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Summary Dendritic cells (DC) are rare, bone marrow-derived antigen-presenting cells that play a critical role in the induction and regulation of immune reactivity. In this article, we review the identification and characterization of liver DC, their ontogenic development, in vivo mobilization and population dynamics. In addition, we discuss the functions of DC isolated from liver tissue or celiac lymph, or propagated in vitro from liver-resident haemopoietic stem/progenitor cells. Evidence concerning the role of DC in viral hepatitis, liver tumours, autoimmune liver diseases, granulomatous inflammation and the outcome of liver transplantation is also discussed.Key words: dendritic cells, immunity, liver, liver disease, tolerance. Identification of hepatic dendritic cells in situIn both rodents and humans, immunohistochemical staining of normal adult livers identifies major histocompatibility complex (MHC) class II + , presumptive dendritic cells (DC) primarily in portal areas, with a few cells scattered throughout the parenchyma. 1-7 The constitutive expression of MHC II antigens (Ag) by these cells, and their dendriform morphology, led to their initial classification as DC. Although MHC class II Ag are also expressed constitutively by human, 7 but not rodent liver macrophages (Kupffer cells), 3 the restricted distribution of DC allows these cells to be readily distinguished from Kupffer cells, which are evident throughout the parenchyma. 1,2,4 MHC class II + presumptive DC have also been described in the liver capsule. 7 In addition to differences in microanatomic location and surface immunophenotype, liver DC and Kupffer cells are distinguished by the absence of non-specific esterase and α-napthylacetate esterase activity in the former cell type. 1,8 Characterization of hepatic dendritic cells in situFew reports have detailed the location of cells immunoreactive with monoclonal Ab (mAb) recognizing DC-associated Ag within the liver. In rats, MHC class II + and/or OX62 + cells are found predominantly in portal areas, with a few positive cells in the sinusoids and around the central vein. 9-11 Immunostaining for ED1 (monocytes/macrophages), ED2 (macrophages/Kupffer cells) and OX6, identifies two distinct phenotypes of hepatic DC: (i) ED1 + ED2 -OX6 + and (ii) ED1 -ED2 -OX6 + . 10 Interestingly, rat hepatic MHC class II + DC appear to express variable levels of CD4, 3,12 which is common in DC in other peripheral tissues of this species. 3,13,14 Mouse CD11c +4 and CD205 + cells 15 are mainly located periportally, as reported for MHC II + cells. The in situ phenotype of human liver DC has been examined quite extensively. 7 Interstitial DC in normal mouse liver exhibit an 'immature' or 'Ag-processing' phenotype, as described for DC in other non-lymphoid tissues. Immunohistochemical staining of normal mouse liver for the costimulatory molecules CD40, CD80 and CD86 16,17 indicates that normal liver DC do not constitutively express detectable amounts of these molecules in situ; a phenotype characteristic of DC in other...

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“…The survival of DDC-depleted grafts is generally prolonged in F1 to parent combinations (5,6,26). Strain-dependent differences in resident dendritic cell numbers (42,43) have been proposed to partly explain this strain-dependent effect of graft dendritic cell depletion (23). In addition, some treatments or manipulations, such as "passive enhancement," to inhibit the immune response of the intermediate recipient during a parking phase, aimed at depleting allogenic resident dendritic cells (5,6), may affect the antigenicity of the transplant and favor carryover of reguloratory cells (see below).…”

Section: Figurementioning

confidence: 97%

Exhaustive Depletion of Graft Resident Dendritic Cells: Marginally Delayed Rejection but Strong Alteration of Graft Infiltration

et al. 2005

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The effects of genetics and age on expression of MHC class II and CD4 antigens on rat cardiac interstitial dendritic cells

Cited by 14 publications

References 16 publications

A histological study on experimental autoimmune myocarditis with special reference to initiation of the disease and cardiac dendritic cells

A histological study on experimental autoimmune myocarditis with special reference to initiation of the disease and cardiac dendritic cells

Immunobiology of liver dendritic cells

Exhaustive Depletion of Graft Resident Dendritic Cells: Marginally Delayed Rejection but Strong Alteration of Graft Infiltration

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