The effects of Gentiana dahurica Fisch on alcoholic liver disease revealed by RNA sequencing

Cao, Houkang; Xi, Shao-Yang; He, Weiwei; Ma, Xuexiao; Liu, Li; Xu, Jie; Zhang, Kefeng; Li, Yingdong; Jin, Ling

doi:10.1016/j.jep.2020.113422

Cited by 13 publications

(5 citation statements)

References 35 publications

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“…Carbohydrates and lipids are the main substances for energy supply in the body, and their metabolic disorder is the main factor leading to lipid accumulation in the liver [ 16 ]. Our previous study confirmed that the most obvious characteristic of mouse liver with ALD was steatosis, which could be reversed by GDEE [ 5 ]. In this study, glucose 6-phosphate, the center of multiple glucose metabolic pathways [ 17 ], was significantly upregulated by alcohol ( Table 1 ).…”

Section: Discussionsupporting

confidence: 74%

“…In our previous study, the results showed that GDEE could inhibit alcohol-induced ALD in mice [ 5 ]. The mice of Control group were given water by gavage for 14 days; the mice of Alcohol group were given 56% alcohol by gavage for 14 days; the mice of Alcohol + GDEE group were given 56% alcohol plus GDEE by gavage for 14 days.…”

Section: Methodsmentioning

confidence: 99%

“…It has become a research hotspot to obtain new drugs for the prevention and treatment of ALD from natural plants, and many studies have demonstrated the safety and effectiveness of natural plant extracts in alleviating ALD [ 4 ]. Our previous study was inspired by the “compendium of materia medica” and clarified the effect of GDEE in alleviating ALD [ 5 ]. In order to further clarify the mechanism of GDEE, this study applied metabolomics technology to reveal the mechanism of GDEE from the metabolite level.…”

Section: Introductionmentioning

confidence: 99%

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A Study on Gentiana dahurica Fisch Ethanol Extract Alleviating Alcoholic Liver Disease in Mice: A Metabolomic Analysis of the Liver

Cao

Guo

Jin

2021

Evidence-Based Complementary and Alternative Medicine

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We clarified the hepatoprotective effect of Gentiana dahurica Fisch ethanol extract (GDEE) in our previous study, and we further revealed the mechanism with the help of metabolomics technology in this study. The livers from Control group, Alcohol group, and Alcohol + GDEE group were analyzed by metabolomics. The metabolites in the liver were separated by ultra-high-performance liquid chromatography (UHPLC) and were tentatively identified using mass spectrometry (MS)/MS analysis. Differential metabolites were defined with VIP > 1 and P < 0.05 . Principal component analysis (PCA) and orthogonal partial least square discriminant analysis (OPLS-DA) were applied to analyze differences among these groups. The results showed that the groups could be clearly distinguished by PCA and OPLS-DA analysis. Alcohol and GDEE could change the overall profile of liver metabolites. Alterations in liver tissues of ALD mice induced by alcohol were mainly involved in the dipeptides, purine and pyrimidine metabolism and glucose and lipid metabolism, which could be partly affected by GDEE. This study revealed that the mechanism of GDEE in alleviating ALD had the characteristics of multitarget and multipathway.

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Section: Discussionsupporting

confidence: 74%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Study on Gentiana dahurica Fisch Ethanol Extract Alleviating Alcoholic Liver Disease in Mice: A Metabolomic Analysis of the Liver

Cao

Guo

Jin

2021

Evidence-Based Complementary and Alternative Medicine

Self Cite

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“…Western medicine can improve liver injury to some extent, but it is easy to tolerate and the clinical effect is not significant [ 11 , 12 ]. Traditional Chinese medicine has a long history in the treatment of ALD and has accumulated a lot of valuable experience and characteristic drugs, which are a great treasure for drug development [ 13 – 15 ].…”

Section: Introductionmentioning

confidence: 99%

Exploring the effect of Yinzhihuang granules on alcoholic liver disease based on pharmacodynamics, network pharmacology and molecular docking

et al. 2023

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Background Yinzhihuang granules (YZHG) is a commonly used Chinese patent medicine for the treatment of liver disease. However, the mechanism of YZHG in alcoholic liver disease (ALD) is still unclear. Methods This study combined liquid chromatography-mass spectrometry technology, pharmacodynamics, network pharmacology and molecular docking methods to evaluate the potential mechanism of YZHG in the treatment of ALD. Results A total of 25 compounds including 4-hydroxyacetophenone, scoparone, geniposide, quercetin, baicalin, baicalein, chlorogenic acid and caffeic acid in YZHG were identified by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The pharmacodynamic investigations indicated that YZHG could improve liver function and the degree of liver tissue lesions, and reduce liver inflammation and oxidative stress in ALD mice. Network pharmacology analysis showed that YZHG treated ALD mainly by regulating inflammation-related signaling pathways such as the PI3K-Akt signaling pathway. The results of the PPI network and molecular docking showed that the targets of SRC, HSP90AA1, STAT3, EGFR and AKT1 could be the key targets of YZHG in the treatment of ALD. Conclusion This study explored the potential compounds, potential targets and signaling pathways of YZHG in the treatment of ALD, which is helpful to clarify the efficacy and mechanism of YZHG and provide new insights for the clinical application of YZHG.

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“…Transcriptomics plays an important role in the research of traditional Chinese medicine because of its holistic and systematic research characteristics at the whole genome transcription level. RNA sequencing technology, which consistent with the concept of TCM, has provide a new perspective for cooperating with a new realization of the mechanisms of drugs and has become an holistic approach that incorporating systems biology, bioinformatics, and polypharmacology to explore and comprehend the mechanisms of TCM [15].…”

Section: Discussionmentioning

confidence: 99%

Cinobufacini Injection Suppresses the Proliferation of Human Osteosarcoma Cells by Inhibiting PI3K/Akt Signaling Pathway

Chen

Zhai

Chen

et al. 2021

Preprint

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Background: Cinobufacini injection (CI), an aqueous extraction from the Cutis Bufonis, is broadly used in clinical treatment of cancer in China. However, the underlying molecular mechanisms of CI in treating osteosarcoma (OS) remain unclear. Aberrant activation of PI3K-AKT signaling pathway is the cause of many types of cancer, including OS. Therefore, we investigated the effect of CI on proliferation, apoptosis and cell cycle of OS cells and elucidated the molecular mechanism of CI in inhibiting OS cells. Methods: Cell proliferation of U2OS and MG63 cells after CI treatment was measured by CCK-8 assay, colony formation and morphological changes. Additionally, the cell cycle arrest and apoptosis induced by CI, were determined by FACS and Western blot analysis. The mechanisms of CI on OS were evaluated by RNA-seq and Western blot analysis. Results: We founf that CI reduced the proliferation of U2OS and MG63 cells in a dose- and time- dependent manner. Furthermore, CI induced the U2OS cells cycle arrest in G0/G1 phase, but the MG63 cells cycle arrest in G2/M phase. Consequently, CI triggered the apoptosis in both OS cells, with enhanced caspase-3 activity and decreased expression of Bcl-2/Bax. In addition, RNA-seq data indicated that PI3K-Akt signaling pathway played an essential role in CI treatment. Moreover PI3K and phosphorylation of AKT (p-AKT) were significantly down-regulated by CI in both OS cells. Conclusions: These results indicate that CI significantly inhibited the proliferation, induced the cell cycle arrest, as well as apoptosis in human OS cells, which is mediated by the inactivation of PI3K-Akt signaling pathway. These findings suggest that CI may have potential for the treatment of OS.

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The effects of Gentiana dahurica Fisch on alcoholic liver disease revealed by RNA sequencing

Cited by 13 publications

References 35 publications

A Study on Gentiana dahurica Fisch Ethanol Extract Alleviating Alcoholic Liver Disease in Mice: A Metabolomic Analysis of the Liver

A Study on Gentiana dahurica Fisch Ethanol Extract Alleviating Alcoholic Liver Disease in Mice: A Metabolomic Analysis of the Liver

Exploring the effect of Yinzhihuang granules on alcoholic liver disease based on pharmacodynamics, network pharmacology and molecular docking

Cinobufacini Injection Suppresses the Proliferation of Human Osteosarcoma Cells by Inhibiting PI3K/Akt Signaling Pathway

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