The effects of GnRHa with and without dopamine antagonist on reproductive hormone levels and ovum viability in tench Tinca tinca

Podhorec, Peter; Socha, Magdalena; Ammar, Imen Ben; Sokolowska-Mikolajczyk, M.; Brzuska, E; Milla, Sylvain; Gosiewski, Grzegorz; Stejskal, Vlastimil; Šimko, Milan; Kouřil, J.

doi:10.1016/j.aquaculture.2016.09.012

Cited by 19 publications

(16 citation statements)

References 44 publications

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“…The values were relatively low in all treatments at the time of ovulation, with medians varying from 0.5 to 7.2 pg/mL, when compared to the values observed at the time of the first dose (with medians ranging from 38.8 to 185.6 pg/mL). These observations pointed out that ovulation was associated with reductions of E 2 in almost all treatments characterizing a phenomenon known as steroidogenic shift known to be associated to successful ovulation with a concomitant and respectively increase and decrease of DHP and E 2 concentrations during induced spawning (Levavi-Zermonsky and Yaron, 1986;Nagahama and Yamashita, 2008;Podhorec et al, 2016;Klangnurak and Tokumoto, 2017;Honji and Moreira, 2017).…”

Section: Discussionmentioning

confidence: 97%

Wild Leporinus friderici induced spawning with different dose of mGnRHa and metoclopramide or carp pituitary extract

Souza

Kuradomi

Rodrigues³

2020

Anim Reprod

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How to cite: Souza TG, Kuradomi RY, Rodrigues SM, Batlouni SR. Wild Leporinus friderici induced spawning with different dose of mGnRHa and metoclopramide or carp pituitary extract. Anim Reprod. 2020;17(1):e20190078. AbstractBreeding technology is of utmost importance for reproduction of wild fish in captivity for the reintroduction and selective breeding programs purposes. The main challenge is that when applied to wild undomesticated specimens, conventional protocols often cause breeders and/or embryo mortality and spawning failure. In this study, we evaluated the reproductive performance of wild Leporinus friderici, a great importance fish for subsistence fishing in South American rivers, applying conventional and lower-dose hormonal therapies by means of two consecutive experiments. In the first, a conventional (0.5 and 5.5 mg/kg) and a lower carp pituitary extract (CPE) dose (0.5 and 1.0 mg/kg) were applied. In the second, a conventional mammalian GnRH analogue associated with metoclopramide (mGnRHa + MET) (40 µg mGnRHa + 20 mg MET/kg) and a lower dose (4 μg mGnRHa + 2 mg MET/kg and 8 µg + 4 mg of mGnRHa + MET/kg) were applied. Ovulation was observed in all treatments, however, only lower CPE protocol provided viable embryos. High levels of 17α,20β-dihydroxy-4-pregnen-3-one (DHP) and 17β estradiol (E 2 ) detected in conventional, but not in lower CPE dose, at ovulation, might be associated to the mortality of the embryos. The use of lower CPE dose applied here was the best way to obtain L. friderici viable embryos. These results directly contribute to the knowledge about poorly explored effects of reproductive management and hormonal therapies in wild-type breeders, showing that the use of reduced doses may be an alternative to reproductive success.

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Section: Discussionmentioning

confidence: 97%

Wild Leporinus friderici induced spawning with different dose of mGnRHa and metoclopramide or carp pituitary extract

Souza

Kuradomi

Rodrigues³

2020

Anim Reprod

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“…The potential of DHP to induce final maturation was demonstrated in several fish species (51)(52)(53)(54). In this study, recombinant GnRH/GAP stimulated a surge of blood DHP occurring h after injection subsequently decline at 24 h. Podhorec (50)…”

Section: Discussionmentioning

confidence: 54%

“…The ability of mGnRHa to induce ovulation goldfish is similar to several species (50). The potential of DHP to induce final maturation was demonstrated in several fish species (51)(52)(53)(54).…”

Section: Discussionmentioning

confidence: 94%

Design, production and purification of a novel recombinant gonadotropin-releasing hormone associated peptide as a spawning inducing agent for fish

Mohammadzadeh

Moradian

Falahatkar

et al. 2020

Protein Expression and Purification

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GnRH is a neuropeptide known to regulate reproduction in vertebrates. The purpose of this study was to design and produce recombinant gonadotropin-releasing hormone associated peptide (rGnRH/GAP) as an alternative of the previous GnRHs and native extracted hormone from tissue, to induce final maturation in fish. Decapeptide as well as GAP area sequences were compared between GnRH1, GnRH2, and mGnRH from Acipenser sp and Huso huso, respectively. Considering the conserved amino acids and the replacement of unstable amino acids with those that were more stable against proteolytic digestion as well as had a longer halflife, the sequence was designed. The sequences of decapeptide and GAP region were synthesized and then cloned on pET28a expression vector and transformed into expression host Escherichia coli BL21(DE3). The supernatant of cultured recombinant bacteria was used for purification using TALON Metal affinity resin. The purity of the GnRH/GAP was confirmed by single 8 kDa band on SDS-PAGE and western blot. Bioinformatics studies were performed for evaluation of homology between GnRH protein sequences and prediction of 3D protein structure using Swiss Model. The result showed that the structure prediction of the recombinant GnRH decapeptide was relatively similar to decapeptide of GnRH2 from Beluga (Huso huso). The GAP structure was similar to GAP1 of Nile tilapia (Oreochromis niloticus) and sturgeon and GnRH2 of Chinese sturgeon (Acipenser sinensis). The mass analysis showed that the sequence was exactly the same as designated sequence. Biology activity of rGnRH/GAP was tested in mature goldfish (Carassius auratus) and results showed that rGnRH/GAP had a positive effect in final maturation. Indeed 17α, 20β-dihydroxy-4-pregnen-3-one (DHP) was increased 17 h and 24h after injection with rGnRH/GAP and spawning stemmed from that injection. These novel findings introduce the potential of utilizing rGnRH/GAP in aquaculture.

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“…Moreover, the intermediate reproductive performance obtained using 1,000 μg sGnRHa/kg highlights that future approaches testing dopamine inhibitors together with sGnRHa may change the current scenario. Even unusual and much higher than that the doses used for hormonal induction for neotropical (Acuña & Rangel, 2009; Pereira et al., 2016, 2018; Souza et al., 2018, 2020; Viveiros et al., 2013), cyprinids (Podhorec et al., 2016; Podhorec, Socha, Sokolowska‐Mikolajczyk, Policar, Svinger, Drozd, et al., 2012; Podhorec, Socha, Sokolowska‐Mikolajczyk, Policar, Svinger, Gosiewski, et al., 2012) and salmonids fishes (Arabaci et al., 2004), the 1,000 μg sGnRHa/kg treatment resulted in fertility and hatching rates above 90%, indicating that the use of this synthetic treatment is not a problem for this species in this concern.…”

Section: Discussionmentioning

confidence: 94%

“…Concerning the ‘steroidogenic shift,’ many studies demonstrated that this shift in steroidogenesis during spawning induction is necessary for final oocyte maturation and ovulation to occur (Chaube et al., 2014; Rahman et al., 2001; Senthilkumaran et al., 2004). In addition, a distinct shift in steroidogenesis is well documented in teleosts by exogenous hormonal therapy (Podhorec et al., 2016; Zadmajid et al., 2017). ‘Steroidogenic shift’ is characterized by a 17,20β‐P level peak during ovulation with a simultaneously decrease in the estradiol (E 2 ) levels (Levavi‐Zermonsky & Yaron, 1986).…”

Section: Discussionmentioning

confidence: 99%

Reproductive performance of lambari ( Astyanax altiparanae ) in a seminatural system using different protocols

et al. 2020

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The effects of GnRHa with and without dopamine antagonist on reproductive hormone levels and ovum viability in tench Tinca tinca

Cited by 19 publications

References 44 publications

Wild Leporinus friderici induced spawning with different dose of mGnRHa and metoclopramide or carp pituitary extract

Wild Leporinus friderici induced spawning with different dose of mGnRHa and metoclopramide or carp pituitary extract

Design, production and purification of a novel recombinant gonadotropin-releasing hormone associated peptide as a spawning inducing agent for fish

Reproductive performance of lambari ( Astyanax altiparanae ) in a seminatural system using different protocols

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