Background/Objectives: The objective of this descriptive research study was to determine effects of caring character and gratitude disposition on nursing professionalism of nursing students.Methods/Statistical analysis: Data were collected from January 10, 2022 to January 23, 2022 using self-reported questionnaires from 110 students in the first to fourth years of nursing education at two universities. Collected data were analyzed using descriptive statistics, t-test, analysis of variance, Pearson's correlation coefficients, and multiple linear regression using the SPSS WINDOW 25.0 program.Findings: Regression analysis revealed that caring character (β=.33, p=.001) and gratitude disposition (β=.26, p=.005) were factors significantly affecting nursing professionalism, with a total explanatory power of 24.9%.Improvements/Applications: To establish and strengthen nursing students' proper nursing professionalism, it is necessary to open courses related to caring character and gratitude disposition with the development of extra-curricular programs for nursing students.