The effects of green synthesized anionic cupric oxide nanoparticles on Zaraibi goat spermatozoa during cryopreservation with and without removal of seminal plasma

Elshamy, Ayat A; Kotram, Laila E; Barakat, Olfat S.; Mahmoud, Sara Mohamed

doi:10.1080/10495398.2022.2106992

Cited by 7 publications

(1 citation statement)

References 69 publications

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“…To maintain sperm quality during the cryopreservation process, it is important to balance the production of ROS and its scavenging. A major strategy for enhancing goat sperm’s resistance to oxidative stress during cryopreservation is the supplementation of exogenous antioxidants in the freezing medium, such as glutathione [ 19 ], green synthesized anionic cupric oxide nanoparticles [ 20 ], Lycium barbarum and Laminaria japonica polysaccharides [ 21 ], turraea fischeri leaf extract [ 22 ], resveratrol and taurine [ 23 ], vitamin E and amino acids [ 24 ], trehalose [ 25 ], myo-inositol and melatonin [ 26 ]. However, most of the exogenous antioxidants are unstable due to oxidation; hence, they lose their anti-oxidative capacity.…”

Section: Introductionmentioning

confidence: 99%

Beneficial Effect of Proline Supplementation on Goat Spermatozoa Quality during Cryopreservation

Zhang

Min

Lang

et al. 2022

Animals

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Sperm cryopreservation contributes to the extensive utilization of artificial insemination (AI) in the daily livestock industry. However, due to the presence of few sperm with good biological function in post-thaw goat sperm, its use has been limited for AI purposes. Hence, its improvement has been the focus of many research studies. This study aimed to investigate the effects of proline supplementation of the freezing medium on goat sperm. The goat semen was cryopreserved with freezing medium supplementation of different concentrations of proline (0, 0.5, 1, 2 and 4 mM). The post-thaw sperm motility patterns, membrane integrity, acrosome integrity, lipid peroxidation (LPO) levels, malondialdehyde (MDA) levels, total antioxidant capacity (T-AOC), proline dehydrogenase (PRODH) activity, superoxide dis-mutase (SOD) activity, glutathione (GSH) levels and GSH/GSSG were evaluated. Likewise, the expression and immunofluorescent localization of PRODH in post-thaw goat sperm was also detected. It was observed that addition of 2 mM proline to the freezing medium significantly enhanced post-thaw goat sperm total motility, progressive motility, straight-linear velocity (VSL), curvilinear velocity (VCL), average path velocity (VAP), straightness (STR), linearity (LIN), membrane integrity and acrosome integrity. Interestingly, PRODH was expressed in post-thaw goat sperm, especially in the post-acrosome and sperm tail. Addition of 2 mM proline also significantly increased the post-thaw sperm PRODH activity compared to the control. Moreover, post-thaw goat sperm LPO levels and MDA levels were reduced by supplementation of 2 mM proline. Furthermore, compared to the control, the values of post-thaw goat sperm T-AOC, SOD activity, GSH level and GSH/GSSG were also significantly increased in 2 mM proline treatment. Reduction of post-thaw goat sperm apoptosis in 2 mM proline treatment was also observed as the levels of Caspase3 and Caspase9 were decreased by the supplementation with 2 mM proline. These observations suggest that the addition of 2 mM proline to the freezing medium increased post-thaw goat sperm quality by reducing oxidative stress during cryopreservation. These findings also provide novel insights into the use of proline as an efficient additive to enhance post-thaw goat sperm quality during cryopreservation.

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