The effects of Haemophilus influenzae vaccination on an aphylactic mediator release and isoprenaline-induced inhibition of mediator release

Abstract

European J. Pharmacol. 62 (1980) 261--268.The influence of Haemophilus influenzae on anaphylactic mediator release from ovalbumin-sensitized isolated guinea pig lungs was investigated. Lungs from H. influenzae-vaccinated animals released prostaglandins and thromboxanes following a smaller dose of ovalbumin than was effective in non-vaccinated animals. Histamine release was significantly increased in 4 day-vaccinated animals but not 1 or 10 days after vaccination, while bronchoconstriction was potentiated in 1 and in 4 day-vaccinated animals. This increased histamine release was achieved following 2 pg ovalbumin. In contrast, doses of 10 pg and 1 mg ovalbumin respectively did not affect and decreased histamine release in the vaccinated group. The inhibition of anaphylactic mediator release by an infusion of 6 × 10 -9 M isoprenaline was significantly attenuated by H. influenzae vaccination. These results indicate an increased sensitivity to antigenic challenge and suggest that the functioning of ~-adrenoceptors was decreased as a result ob H. influenzae vaccination. Haemophilus influenzae HistamineAnaphylactic shock Prostaglandins Thromboxane A2

Anterior hypothalamic lesions prevent the endotoxin-induced reduction of beta-adrenoceptor number in guinea pig lung

“…Several gram negative bacilli have been shown to decrease the number as well as the functioning of beta-adrenoceptors in the guinea pig lung and it appeared that the endotoxin (LPS) of the bacterial cell wall was responsible for this phenomenon 18,19,21. Endotoxin has been shown to have strong immunogenic properties1.…”

Effects of dietary linoleic acid on beta-adrenergic responsiveness of the guinea pig respiratory system

“…The method used is a modification of that described earlier (2,3). Immediately after removal of the tracheae, lungs were flushed twice through the pulmonary artery with 30 ml Krebs bicarbonate and dissected free of major bronchi.…”

“…The function of atory tract for a variety of stimuli. An imbalance the P-adrenergic receptor-adenylate cyclase between the bronchoconstrictive ar-adrenergic system is also impaired, causing inadequate and cholinergic receptor system and the bron-physiological responses (2)(3)(4). Recently it was chodilatory p-adrenergic receptor system may postulated that the cellular defect in asthmatic contribute to the bronchial hyperreactivity (1).…”

“…Recently it was chodilatory p-adrenergic receptor system may postulated that the cellular defect in asthmatic contribute to the bronchial hyperreactivity (1). patients was not located in the adrenergic or We previously showed that administration of the other receptors themselves but somewhere in the Gramnegative bacterium Haemophilus influenzae subsequent biochemical steps that link the actito guinea pigs gave rise to responses comparable vation of the receptor to the final cellular events to those found in atopic patients (2)(3)(4). The under consideration, i.e.…”