The effects of hydroalcoholic extract of spinach on prevention and treatment of some metabolic and histologic features in a rat model of nonalcoholic fatty liver disease

Abstract

BACKGROUND: This study evaluated the effects of hydroalcoholic extract of spinach (HES) on nonalcoholic fatty liver disease (NAFLD). In the prevention phase, 18 Sprague-Dawley rats were fed a high-fat diet, a high-fat diet plus 400 mg kg −1 HES, or a chow diet for 7 weeks. For the treatment phase, after the induction of NAFLD, they were fed a high-fat diet, a high-fat diet plus 400 mg kg −1 HES, a chow diet, or chow diet plus 400 mg kg −1 HES for 4 weeks (n = 6).RESULTS: Weight gain (P = 0.01), food intake (P < 0.01), serum glucose (P = 0.01), triglyceride (TG) (P = 0.02), low-density lipoprotein cholesterol (LDL-c) (P = 0.01), aspartate aminotransferase (AST) (P = 0.02), liver steatosis, and the nonalcoholic fatty liver disease (NAFLD) activity score (NAS) (P < 0.01) in the high-fat group were statistically higher than in the other groups at the end of the prevention phase. Feeding spinach extract to rats on a high-fat diet decreased serum glucose (P = 0.01), total cholesterol (TCh) (P < 0.01), AST (P = 0.01), alkaline phosphatase (ALP) (P < 0.01), and liver steatosis (P < 0.01) in the treatment phase.CONCLUSION: Overall, spinach extract showed beneficial effects in the prevention and treatment of NAFLD.

Bioavailability and biotransformation of linolenic acid from basil seed oil as a novel source of omega-3 fatty acids tested on a rat experimental model

“…40 In addition, an overall absence of liver damage caused by BSO intake could be inferred from the low plasma activity values of transaminases. The slight increase in ALT and the 2.2-fold increment in ALP levels for the BSO 15% group could be attributed to the increase in the dietary fat levels 24,41,42 and could indicate a potential adverse effect of the higher oil dose assayed in this study, as has been reported for studies involving different dietary oils 43 but not for the dose within the range of values that meet the nutrient requirements of the experimental animal used. 25…”

Banana green peels extract protects against nonalcoholic fatty liver disease in high‐fat‐fed mice through modulation of lipid metabolism and inflammation

“…Under the umbrella of chronic diseases related to obesity, nonalcoholic fatty liver disease (NAFLD) has been considered a major public health problem (Estes et al, 2018; Younossi, 2019). Although there are currently no specific drugs registered for this disease, insulin sensitizers and antioxidants have shown to be useful in its treatment, including those found in natural products (Amirinejad et al, 2021; Amirinejad, Hekmatdoost, Ebrahimi, Ranjbaran, & Shidfar, 2020; Simental‐Mendía et al, 2021). Considering the beneficial effects of banana peels on metabolic responses and the limited number of scientific studies focused on its biological activities, the present study investigated the effects of banana green peels extract (BPE) as a preventive treatment for dyslipidemia and insulin resistance, evaluating its beneficial action against NAFLD establishment in obese mice fed high‐fat diet.…”

Administration of hydro-alcoholic extract of spinach improves oxidative stress and inflammation in high-fat diet-induced NAFLD rats

“…Consumption of spinach decreased liver steatosis and improved glucose and lipid profile in previous animalbased NAFLD models [28,29]. To further establish the existing data, this study aimed to investigate the effect of supplementation with hydro-alcoholic extract of spinach (HES) on serum levels and hepatic gene expression of some inflammatory and oxidative factors in a rat model of NAFLD.…”