The Effects of<i>Costaria costata</i>Extracts on Atopic Dermatitis in an<i>In Vitro</i>Model

Kim, Ok‐Kyung; Nam, Da‐Eun; Lee, Minhee; Kwon, Han Ol; Park, Jeongjin; You, Yanghee; Kim, Su‐Il; Lee, Jeongmin; Jun, Woojin

doi:10.1089/jmf.2016.3691

Cited by 5 publications

(7 citation statements)

References 30 publications

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“…PMA and A23187 used for activating MC/9 mast cells in our study are highly potent PKC activator and calcium ionophore, respectively [44]. In several reports, PMA and A23187 treatment significantly elevated the histamine release in MC/9 mast cells [45,46]. Therefore, we used PMA and A23187 as activators for degranulation of MC/9 mast cells in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Kim demonstrated the effects of Costaria costata extract on atopic dermatitis via suppressing expression of TARC, eotaxin, TNF-α, and IL-6 in TNF-α and IFN-γ-stimulated HaCaT keratinocytes as well as release histamine in PMA and A23187-stimulated MC/9 mast cells [45]. In our results, PMA and A23187 induced the activation of 5-LO and the production of histamine and LTC 4 in MC/9 mast cells.…”

Section: Discussionmentioning

confidence: 99%

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Anti-Allergic and Anti-Inflammatory Effects of Kuwanon G and Morusin on MC/9 Mast Cells and HaCaT Keratinocytes

Jin

Shin

et al. 2019

Molecules

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Atopic dermatitis (AD) is a prevalent chronic inflammatory skin disease. The use of immunomodulatory corticosteroids in AD treatment causes adverse side effects. Therefore, novel natural anti-inflammatory therapeutics are needed. The aim of the present study was to investigate the anti-allergic and anti-inflammatory activities of kuwanon G and morusin. To investigate the effect of kuwanon G and morusin on skin inflammation, enzyme-linked immunosorbent assays (ELISA) to quantitate secreted (RANTES/CCL5), thymus- and activation-regulated chemokine (TARC/CCL17), and macrophage-derived chemokine (MDC/CCL22) were performed, followed by Western blotting to measure the phosphorylation of signal transducer and activator of transcription 1 (STAT1) and nuclear transcription factor-κB (NF-κB) p65 in tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ)-stimulated HaCaT keratinocytes. In order to evaluate the anti-allergic effects, ELISA to quantify histamine and leukotriene C4 (LTC4) production and Western blotting to measure 5-lipoxygenase (5-LO) activation were performed using PMA and A23187-stimulated MC/9 mast cells. Kuwanon G reduced the release of RANTES/CCL5, TARC/CCL17, and MDC/CCL22 via down-regulation of STAT1 and NF-κB p65 signaling in TNF-α and IFN-γ-stimulated HaCaT keratinocytes. Kuwanon G also inhibited histamine production and 5-LO activation in PMA and A23187-stimulated MC/9 mast cells. Morusin inhibited RANTES/CCL5 and TARC/CCL17 secretion via the suppression of STAT1 and NF-κB p65 phosphorylation in TNF-α and IFN-γ-stimulated HaCaT keratinocytes, and the release of histamine and LTC4 by suppressing 5-LO activation in PMA and A23187-stimulated MC/9 mast cells. Kuwanon G and morusin are potential anti-inflammatory mediators for the treatment of allergic and inflammatory skin diseases such as AD.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Anti-Allergic and Anti-Inflammatory Effects of Kuwanon G and Morusin on MC/9 Mast Cells and HaCaT Keratinocytes

Jin

Shin

et al. 2019

Molecules

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“…The intensity of fluorescence was measured at 490 nm and 520 nm using a microplate reader. PK Solver 2.0 and non-atrioventricular model were used to analyze the pharmacokinetic parameters of the experiment [30,31].…”

Section: Tissue Distribution Of Fucoidan In Micementioning

confidence: 99%

Study on Absorption Mechanism and Tissue Distribution of Fucoidan

Bai

Zhang

et al. 2020

Molecules

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Fucoidan exhibits several pharmacological activities and is characterized by high safety and the absence of toxic side effects. However, the absorption of fucoidan is not well-characterized. In the present study, fucoidan were labeled with fluorescein isothiocyanate (FITC) and their ability to traverse a monolayer of Caco-2 cells was examined. The apparent permeability coefficients (Papp × 10−6) of FITC-labeled fucoidan (FITC-fucoidan) were 26.23, 20.15, 17.93, 16.11 cm/sec, respectively, at the concentration of 10 μg/mL at 0.5, 1, 1.5 and 2 h. The absorption of FITC-fucoidan was suppressed by inhibitors of clathrin-mediated endocytosis, chlorpromazine, NH4Cl, and Dynasore; the inhibition rates were 84.24%, 74.61%, and 63.94%, respectively. This finding suggested that clathrin-mediated endocytosis was involved in fucoidan transport. Finally, tissue distribution of FITC-fucoidan was studied in vivo after injection of 50 mg/kg body weight into the tail vein of mice. The results showed that FITC-fucoidan targeted kidney and liver, reaching concentrations of 1092.31 and 284.27 μg/g respectively after 0.5 h. In summary, the present work identified the mechanism of absorption of fucoidan and documented its tissue distribution, providing a theoretical basis for the future development of fucoidan applications.

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“…In a previous study, thymus and activation-regulated chemokine (TARC) and regulated on activation, and normal T cell expressed and secreted (RANTES) were shown to play a key role in the pathogenesis of AD. In addition, TGF-b1 is known to modulate immune system [19][20][21][22][23]. PE treatment decreased the levels of TARC and RANTES mRNAs (which were increased by TNFα/IFN-γ) in a dose-dependent manner (Fig.…”

Section: Regulation Of Ad-related Gene Expression In Tnfα/ifn-γ-stimu...mentioning

confidence: 93%

The Inhibitory Effect of Human Placenta Extract (Laennec ® ) on Atopic Dermatitis-Like Skin Disease In Vivo and In Vitro

Jeong

Jeon

Jing

et al. 2023

Preprint

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Atopic dermatitis (AD) is an allergic inflammatory skin disease accompanied by severe itching and eczema. Human placenta extract (PE), used for treatment of conditions related to hepatic function, possess anti-inflammatory and antiviral properties. In this study, we investigated the efficacy of PE against AD in a 2,4-dinitrochlorobenzene (DNCB)-induced AD mouse model, and in tumor necrosis factor (TNF)-α/interferon (IFN)-γ-stimulated human keratinocytes (HaCaT) and immunized splenocytes. We confirmed that subcutaneous administration of PE (200 or 400 µL, twice a week) improved AD skin lesions and reduced the spleen index in mice. In addition, the expression levels of immune-related and proinflammatory factors were suppressed in serum and skin tissue. These results were consistent with the tendency observed in HaCaT cells and splenocytes. Intriguingly, human-derived PE had no side effects, such as loss of muscle mass, compared to corticosteroid, which was used as a positive control. Taken together, our results demonstrate that PE effectively inhibits the development of AD and might be a potentially useful therapeutic agent for AD-like skin disease.

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The Effects ofCostaria costataExtracts on Atopic Dermatitis in anIn VitroModel

Cited by 5 publications

References 30 publications

Anti-Allergic and Anti-Inflammatory Effects of Kuwanon G and Morusin on MC/9 Mast Cells and HaCaT Keratinocytes

Anti-Allergic and Anti-Inflammatory Effects of Kuwanon G and Morusin on MC/9 Mast Cells and HaCaT Keratinocytes

Study on Absorption Mechanism and Tissue Distribution of Fucoidan

The Inhibitory Effect of Human Placenta Extract (Laennec ® ) on Atopic Dermatitis-Like Skin Disease In Vivo and In Vitro

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