The effects of Hericium erinaceus extracts on cell viability and telomerase activity in MCF-7 cells

Abstract

Objectives This study aims to determine the effects of Hericium erinaceus extracts on cell viability and the effects of H. erinaceus water extract on the telomerase activity of MCF-7 cells. H. erinaceus is an edible mushroom widely used in traditional Chinese medicine. Although its various therapeutic properties, the literature has not yet submitted evidence about H. erinaceus for its effects on the telomerase activity of MCF-7 cells. Methods MCF-7 cells were treated with ethanol, ethanol-water, ether, ethyl acetate, methanol-water, and water extracts to determine the effects on cell viability using the WST8 method. The TeloTAGGG Telomerase PCR ELISA kit was used to assess telomerase activity. Results The water extract was determined to be the most efficient extract to decrease cell viability. The water extract’s half-maximal inhibitory concentration was 250 μg/mL at 72 h. It is found that H. erinaceus has no statistically significant effect compared to positive control on reducing telomerase activity. We found a statistically significant difference in telomerase activity % between H. erinaceus water extracts and negative control (p<0.05). Conclusions Consequently, these differences in telomerase activity are a significant association rather than inferring action. It is considered that water extract shows its cell viability inhibition effects through different mechanisms.

Biological Activities of Ethanol Extracts of Hericium erinaceus Obtained as a Result of Optimization Analysis

“…In a previous study conducted in China, it was reported that water and ethanol extracts of H. erinaceus had antiproliferative activity on HepG2, Huh-7, HT-29 and NCI-87 cell lines [18]. In a different study conducted in Turkey, it was reported that H. erinaceus' ethanol-water, ethanol, methanol-water, water, ethyl acetate, and ether extracts had antiproliferative activity against MCF-7 cells [19]. In our study, ethanol extracts of H. erinaceus were used and their activity against the A549 cancer cell line was tested.…”