The Effects of Indomethacin and Thiorphan on Bradykinin-induced Albumin Output and Submucosal Gland Secretion in the Ferret Trachea in vivo

Wells, U.; Yurdakos, E.; Kitano, Soichi; Webber, Stephanie; Widdicombe, J. G.

doi:10.1006/pulp.1993.1017

Cited by 3 publications

(2 citation statements)

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“…In intact glands, cholinergic, ␣-adrenergic, and ␤-adrenergic agents all stimulate lysozyme secretion (42,46), as do several other neurohumoral agents (9,47). Furthermore, this stimulation is associated with serous cell degranulation (42).…”

Section: L752mentioning

confidence: 97%

Secretion of lactoferrin and lysozyme by cultures of human airway epithelium

Dubin

Robinson

Widdicombe

2004

American Journal of Physiology-Lung Cellular and Molecular Phys

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Lactoferrin and lysozyme are important antimicrobial compounds of airway surface liquid, derived predominantly from serous cells of submucosal glands but also from surface epithelium. Here we compared release of these compounds from the following human cell cultures: primary cultures of tracheal epithelium (HTE), Calu-3 cells (a lung adenocarcinoma cell line frequently used as a model of serous gland cells), 16HBE14o- cells (an SV40 transformed line from airway surface epithelium), T84 cells (a colon carcinoma cell line), and human foreskin fibroblasts (HFF). For lysozyme, baseline secretory rates were in the order Calu-3 > 16HBE14o- > HTE T84 > HFF = 0; for lactoferrin, the only cell type showing measurable release was HTE; for mucus, HTE > Calu-3 > 16HBE14o- T84 > HFF = 0. A wide variety of neurohumoral agents and inflammatory stimuli was without effect on lactoferrin and lysozyme release from HTE or Calu-3 cells, although forskolin did stimulate secretion of water and lysozyme from Calu-3 cells. However, the concentration of lysozyme in the forskolin-induced secretions was much less than in airway gland secretions. Thus our data cast doubt on the utility of Calu-3 cells as a model of airway serous gland cells but do suggest that HTE could prove highly suitable for studies of mucin synthesis and release.

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Section: L752mentioning

confidence: 97%

Secretion of lactoferrin and lysozyme by cultures of human airway epithelium

Dubin

Robinson

Widdicombe

2004

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…Elevated levels of albumin in ASL are thought to contribute to the tenacity of mucus in asthma (288). Albumin is actively transported into the lumen of ferret tracheas in vitro, and agents that stimulate ferret gland secretion also increase the secretion of albumin (718,723,724,765). Because similar results were obtained with gland-free rabbit tracheas (526), it was unclear whether airway glands secrete albumin.…”

Section: Proteins Peptides and Small Organic Moleculesmentioning

confidence: 98%

Airway Gland Structure and Function

Widdicombe

Wine

2015

Physiological Reviews

180

221

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Submucosal glands contribute to airway surface liquid (ASL), a film that protects all airway surfaces. Glandular mucus comprises electrolytes, water, the gel-forming mucin MUC5B, and hundreds of different proteins with diverse protective functions. Gland volume per unit area of mucosal surface correlates positively with impaction rate of inhaled particles. In human main bronchi, the volume of the glands is ∼ 50 times that of surface goblet cells, but the glands diminish in size and frequency distally. ASL and its trapped particles are removed from the airways by mucociliary transport. Airway glands have a tubuloacinar structure, with a single terminal duct, a nonciliated collecting duct, then branching secretory tubules lined with mucous cells and ending in serous acini. They allow for a massive increase in numbers of mucus-producing cells without replacing surface ciliated cells. Active secretion of Cl(-) and HCO3 (-) by serous cells produces most of the fluid of gland secretions. Glands are densely innervated by tonically active, mutually excitatory airway intrinsic neurons. Most gland mucus is secreted constitutively in vivo, with large, transient increases produced by emergency reflex drive from the vagus. Elevations of [cAMP]i and [Ca(2+)]i coordinate electrolyte and macromolecular secretion and probably occur together for baseline activity in vivo, with cholinergic elevation of [Ca(2+)]i being mainly responsive for transient increases in secretion. Altered submucosal gland function contributes to the pathology of all obstructive diseases, but is an early stage of pathogenesis only in cystic fibrosis.

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Control of Tracheobronchial Microvascular Permeability

Pedersen

Goldie

1994

Airways Smooth Muscle: Development, and Regulation of Contractility

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The Effects of Indomethacin and Thiorphan on Bradykinin-induced Albumin Output and Submucosal Gland Secretion in the Ferret Trachea in vivo

Cited by 3 publications

References 0 publications

Secretion of lactoferrin and lysozyme by cultures of human airway epithelium

Secretion of lactoferrin and lysozyme by cultures of human airway epithelium

Airway Gland Structure and Function

Control of Tracheobronchial Microvascular Permeability

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