The effects of insulin on glucose and fluid transport in the isolated small intestine of normal rats

Asl, Saleh Zahedi; Alipour, Mohammad Reza

doi:10.1016/j.lfs.2007.04.021

Cited by 5 publications

(4 citation statements)

References 43 publications

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“…The everted rat small intestine experiment was approved by the Ethical Review Committee of Jiangnan University. The experiment was performed as described by Zahedi and Alipour and Tomimatsu and Horie, with slight modifications. A jejunum segment (10 cm in length), which was cut about 15 cm below the pylorus, was used for the everted sac experiment.…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies have shown that the active transport of glucose by glucose transporters in the small intestine can be influenced by many hypoglycaemic factors such as polyphenol, insulin and gastrointestinal motility . Makelainen et al hypothesised that OBG may form a protective layer along the small intestinal wall, acting as a viscous barrier to decrease glucose transportation.…”

Section: Introductionmentioning

confidence: 99%

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The effect of oat β‐glucan on in vitro glucose diffusion and glucose transport in rat small intestine

et al. 2015

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The effect of oat β‐glucan on in vitro glucose diffusion and glucose transport in rat small intestine

et al. 2015

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“…Thus, glucose transport is an example of secondary active transport, provided indirectly by the active transport of sodium out of the cell, which maintains the concentration gradient across the luminal border of the cell. Glucose then passively passes from the enterocytes to the circulation through glucose transporter 2 (GLUT-2) in the basolateral membrane [21]. The Na + /H + exchange which occurs by NHE1 found in basolateral membrane, NHE2 and NHE3 both at the brush border membrane of the intestinal epithelium [22][23][24][25] and Na + absorption through the Na + /Clco-transporter [22] are alternative sodium absorption pathways.…”

Section: Resultsmentioning

confidence: 99%

Does pH of tyrode solution modify glucose and electrolyte jejunal absorption in rats?

Borges¹,

Viana²

2012

JBPC

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Studies have shown that glucose is able to decrease the pH of the surface epithelium jejunal preparations when added <i>in vitro</i> and the existence of a high concentration of protons in the immediate area of the mucosa could be of considerable significance for absorption of electrolytes. The aim of this study is to assess whether the change in pH of Tyrode (solution used for perfusion of the jejunum) interferes with the absorption of glucose and electrolytes. Male Wistar rats weighing 200 to 220 g (n = 6) were utilized. Jejunal absorption of glucose and electrolytes was investigated in rats. A Tyrode solution containing twice glucose, sodium and potassium concentration (pH 7.0, 7.4, 8.0 and 8.5) was infused through the jejunal loops during 40 minutes. The glucose absorption was not significantly affected by Tyrode. However, there was significantly decrease in sodium absorption at pH 7.0 and 8.5 (41.13 ± 2.79 and 41.37 ± 1.71, respectively, P < 0.05) when compared with the uptake at pH 7.4 and 8.0 (61.06 ± 6.50 and 56.28 ± 7.03, respectively, P < 0.05). Moreover, potassium absorption increased at pH 8.0 (1.04 ± 0.07) when compared with the uptake at pH 7.0 (0.59 ± 0.04), 7.4 (0.78 ± 0.08) and 8.5 (0.54 ± 0.05) (P < 0.05). These data indicate that the pH of Tyrode has no influence on glucose absorption. However, the major potassium uptake occurs at pH 8.0, while the absorption of sodium is impaired at pH 7.0 and 8.5

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“…Briefly, each sac (4 sacs for each condition) was made by tying one end of the segment, filling it with 1 ml/10 cm (of the segment) of Krebs bicarbonate solution (containing 120 mM NaCl, 4.5 mM KCl, 1 mM MgSO 4 1.8 mM Na2HPO4, 0.2 mM NaH2PO4, 1.25 mM CaCl2, 25 mM NaHCO3, and 5.5 mM glucose) and then tying the other end. The sacs were immersed in 40 ml of Krebs solution (containing 120 mM NaCl, 4.5 mM KCl, 1 mM MgSO 4 1.8 mM Na2HPO4, 0.2 mM NaH2PO4, 1.25 mM CaCl2, 25 mM NaHCO3, and 30 mM glucose) in a flask and incubated in a shaker bath (30 min, 37°C 40 rpm) (43). The sacs were then removed, blotted, weighed, and opened, and the changes of glucose concentration in their contents (serosal fluid) and the incubating media (mucosal fluid) were analyzed as described above.…”

Section: Measurement Of Intestinal Glucose Transport Ex Vivomentioning

confidence: 99%

Estriol blunts postprandial blood glucose rise in male rats through regulating intestinal glucose transporters

Yamabe

Kang

Lee

et al. 2015

American Journal of Physiology-Endocrinology and Metabolism

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Despite increased total food intake in healthy, late-stage pregnant women, their peak postprandial blood sugar levels are normally much lower than the levels seen in healthy nonpregnant women. In this study, we sought to determine whether estriol (E3), an endogenous estrogen predominantly produced during human pregnancy, contributes to the regulation of the postprandial blood glucose level in healthy normal rats. In vivo studies using rats showed that E3 blunted the speed and magnitude of the blood glucose rise following oral glucose administration, but it did not appear to affect the total amount of glucose absorbed. E3 also did not affect insulin secretion, but it significantly reduced the rate of intestinal glucose transport compared with vehicle-treated animals. Consistent with this finding, expression of the sodium-dependent glucose transporter 1 and 2 was significantly downregulated by E3 treatment in the brush-border membrane and basolateral membrane, respectively, of enterocytes. Most of the observed in vivo effects were noticeably stronger with E3 than with 17β-estradiol. Using differentiated human Caco-2 enterocyte monolayer culture as an in vitro model, we confirmed that E3 at physiologically relevant concentrations could directly inhibit glucose uptake via suppression of glucose transporter 2 expression, whereas 17β-estradiol did not have a similar effect. Collectively, these data showed that E3 can blunt the postprandial glycemic surge in rats through modulating the level of intestinal glucose transporters.

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The effects of insulin on glucose and fluid transport in the isolated small intestine of normal rats

Cited by 5 publications

References 43 publications

The effect of oat β‐glucan on in vitro glucose diffusion and glucose transport in rat small intestine

The effect of oat β‐glucan on in vitro glucose diffusion and glucose transport in rat small intestine

Does pH of tyrode solution modify glucose and electrolyte jejunal absorption in rats?

Estriol blunts postprandial blood glucose rise in male rats through regulating intestinal glucose transporters

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