The effects of L-carnitine on sperm parameters in smoker and non-smoker patients with idiopathic sperm abnormalities

Khademi, Ali; Alleyassin, Ashraf; Safdarian, Leili; Hamed, Ehsan Akbari; Rabiee, Ehsan; Haghaninezhad, Hasan

doi:10.1007/s10815-005-7204-4

Cited by 13 publications

(8 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although the D100 rats have shown altered sperm transit time, as formerly mentioned, the carnitine-doxorubicin-treated rats showed normal values in various sperm parameters, including the caput/corpus sperm transit time. These data agree with previously published studies that mentioned an improvement of the spermatic parameters in infertile patients after carnitine treatment (Ng et al, 2004;Agarwal & Said, 2004;Kadhemi et al 2005).…”

Section: Discussionsupporting

confidence: 93%

Carnitine partially protects the rat testis against the late damage produced by doxorubicin administered during pre‐puberty

et al. 2014

View full text Add to dashboard Cite

SUMMARYDoxorubicin, an anticancer drug, is widely included in chemotherapy protocols to combat childhood cancer. Carnitine, an important quaternary amine, is present in testis and epididymis and is involved in sperm maturation; it has been used in infertility treatment. In a previous study, our group observed that L-carnitine given before etoposide, another chemotherapeutic drug, reduces the spermatogenic damage and protects germ cells against apoptosis. This study aimed to evaluate the antiapoptotic and cytoprotective actions of L-carnitine in long-and mid-term basis, on the seminiferous epithelium of doxorubicin-treated pre-pubertal rats. Fortyeight 30-day-old male Wistar rats were distributed into four groups: sham-control; doxorubicin; carnitine; carnitine/doxorubicin (L-carnitine injected 1 h before doxorubicin). The rats were submitted to euthanasia at 64 and 100 days of age and their testes were collected for biometric, morphometric, and histopathological analyses. The numerical density of apoptotic germ cells was obtained (TUNEL method). In adult phase (100 days), the following spermatic parameters were analyzed: mature spermatid (19 step) count and sperm daily production per testis; sperm number and transit time through the epididymal caput/corpus and cauda; frequency of morphologically abnormal spermatozoa (from epididymal fluid), as well as sperm DNA integrity (Comet assay). The testicular and spermatic parameters at both ages were improved in rats treated with carnitine before doxorubicin. At 64 days, the TUNEL-positive germ cell frequency was lower in the carnitine/doxorubicin-treated rats comparatively to the doxorubicin-treated rats. At 100 days of age, the sperm DNA fragmentation was also lower in the previously carnitine-treated rats, as evidenced by the analysis of three parameters. Carnitine reduced the late testicular and spermatic damages caused by doxorubicin, probably providing a partial cytoprotection against the deleterious action of doxorubicin administration to pre-pubertal rats. However, further studies shall be undertaken to investigate the protective mechanisms involved in such germ cell preservation.

show abstract

Section: Discussionsupporting

confidence: 93%

Carnitine partially protects the rat testis against the late damage produced by doxorubicin administered during pre‐puberty

et al. 2014

View full text Add to dashboard Cite

show abstract

“…2005). Oral l ‐carnitine (1 g of l ‐carnitine orally 3 × daily for 3 months) was effective in improving semen quality in men (Khademi et al. 2005).…”

Section: Addressing Individual Differences In Cryotolerancementioning

confidence: 99%

“…L-carnitine stabilizes the mitochondrial membrane, increases supply of energy of the organelles and protects cells from apoptotic cell death (Pillich et al 2005). Oral L-carnitine (1 g of L-carnitine orally 3 · daily for 3 months) was effective in improving semen quality in men (Khademi et al 2005). Acidic seminal fluid protein found in bull seminal plasma can protect spermatozoa from oxidative damage (Schoeneck et al 1996), whereas seminal fluid steroids, such as dihydroepiandrosterone and some of its metabolites that have antioxidative properties, have been detected in human seminal fluid (Hampl et al 2003).…”

Section: Antioxidant Supplementationmentioning

confidence: 99%

Cryopreservation of Canine Semen – New Challenges

Farstad

2009

Reprod Domestic Animals

View full text Add to dashboard Cite

Egg yolk (EY) protects cell membranes against cold shock, and it prevents or restores the loss of phospholipids from the membrane. EY has been widely used in semen extenders. It has been added to Tris-Glucose buffer and has been widely used for cooling and cryopreservation of canine semen. EY is not a defined entity, but a complex biological compound containing proteins, vitamins, phospholipids, glucose and antioxidants which are all potentially useful for cell membrane integrity. Unfortunately, it also is a biologically hazardous compound. Hence, whole EY needs to be replaced by other chemically defined components for semen processing in dogs. Freezing poor semen does not improve its quality, so attention must be focused on how to cope with dogs whose semen does not freeze well, and on designing individual freezing extenders for semen from such males. Furthermore, differences have been found among canid species in the ability of their spermatozoa to withstand freezing. There are differences in sperm membrane fatty acid composition among species, which may explain part of these differences. If the presence of long-chained polyunsaturated fatty acids contributes to increased membrane fluidity, this relationship may be biphasic, i.e. either too much membrane fluidity, or too little, could compromise successful sperm cryopreservation. An increase in fluidity of the outer leaflet of the plasma membrane has been shown in frozen thawed dog spermatozoa. The protective effect of exogenous lipids may lie in close association with the membrane rather than in modification or rearrangement of the membrane. This also points at lipids as an important, if not entirely new group of substances, which may substitute standard EY-based diluents in preserving sperm survival during freezing. EY-derived phospholipids or lecithin could be used to replace whole EY. Vegetable lecithin is currently investigated to avoid using substances of animal origin. EY also contains antioxidants which prevent cells from oxidative damage due to the generation of reactive oxygen species. An increasing number of publications now recognize the significance of protecting sperm from this damage during processing by using dietary or diluent supplemented antioxidants. This paper aims at looking at some of the new challenges in freezing of dog semen.

show abstract

“…Our study indicated that the percentage of motile spermatozoa (grades A + B) is increased from 27.8% to 35.2% after 10 min treatment with 0.5 mmol/L l ‐carnitine, which is consistent with the result of Banihani (Banihani, Agarwal, Sharma, & Bayachou, ; Banihani, Sharma, Bayachou, Sabanegh, & Agarwal, ; Figure a,b). However, the influence of l ‐carnitine on sperm morphology (Khademi et al, ) and other parameters remains to be clarified.…”

Section: Discussionmentioning

confidence: 99%

The influence of l ‐carnitine on the expression of miRNAs in asthenospermia spermatozoa and the network regulation of the associated molecules

Liu

et al. 2019

Andrologia

View full text Add to dashboard Cite

l‐carnitine is a natural compound that is indispensable for energy metabolism in mammals. The efficiency and safety of l‐carnitine in improving sperm activity, enhancing epididymal function and treating male infertility has been widely acknowledged by clinicians. CircRNAs can regulate gene expression at the transcriptional or post‐transcriptional level by serving as a molecular sponge of miRNAs with miRNA response elements. However, the detailed mechanism linking miRNA, circRNA and asthenospermia remains unclear. The present study demonstrated that hsa‐miR‐27b‐3p, hsa‐miR‐151a‐5p and hsa‐miR‐206 play an important role in the effects of l‐carnitine treatment of the spermatozoa in asthenospermia patients. Furthermore, the target mRNAs of hsa‐miR‐206 were analysed by GO and KEGG. The results show that the target mRNAs of hsa‐miR‐206 may change the activity of ATP synthase and participate in the cAMP signalling pathway and the calcium signalling pathway, which may play an important role in sperm motility.

show abstract

The effects of L-carnitine on sperm parameters in smoker and non-smoker patients with idiopathic sperm abnormalities

Abstract: The results of this study indicate that smoker patients should not be excluded from the treatment with L-carnitine based on smoking alone.

Cited by 13 publications

References 17 publications

Carnitine partially protects the rat testis against the late damage produced by doxorubicin administered during pre‐puberty

Carnitine partially protects the rat testis against the late damage produced by doxorubicin administered during pre‐puberty

Cryopreservation of Canine Semen – New Challenges

The influence of l ‐carnitine on the expression of miRNAs in asthenospermia spermatozoa and the network regulation of the associated molecules

Contact Info

Product

Resources

About