The Effects of Lactobacillus plantarum-12 Crude Exopolysaccharides on the Cell Proliferation and Apoptosis of Human Colon Cancer (HT-29) Cells

Sun, Mengying; Liu, Wenwen; Song, Yinglong; Mu, Guangqing; Ma, Fenglian

doi:10.1007/s12602-020-09699-8

Cited by 38 publications

(31 citation statements)

References 45 publications

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“…Interestingly, at the highest concentration and after 48 h of incubation, the inhibition rates of HT-29 cell line proliferation increased significantly (p < 0.01) up to 66 and 69% for iEPS-1.0 and iEPS-1.5 kGy, respectively, and they were similar to the positive control 5-fluorouracil molecule (5-FU) (71.2 ± 0.23% at 24 h incubation period). In comparison to previous research findings, the iEPSs inhibition rate against the HT-29 cell line was three-fold better than that obtained by crude EPS from L. plantarum-12 at the same dose of 250 µg mL −1 (28.4 ± 1.37) [64] and two-fold better than that of EPS from L. casei SB27 (35.98 ± 0.47) [65]. The irradiation-induced increase in the anti-proliferative activity of iEPSs could be attributed to their low MW as well as to physicochemical changes of the nEPS by radiolysis, as demonstrated above by different analytical tools.…”

Section: Antitumor Activities Of Neps and Iepsscontrasting

confidence: 78%

Effect of Gamma Irradiation on Enhanced Biological Activities of Exopolysaccharide from Halomonas desertis G11: Biochemical and Genomic Insights

Chouchane¹,

Boutiti²,

Ouertani³

et al. 2021

Polymers

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In this work, a native exopolysaccharide (nEPS) produced by Halomonas desertis G11 isolated from a Tunisian extreme environment was modified by gamma irradiation. Characterization as well as the antioxidant and antitumor activities of nEPS and its gamma-irradiated derivatives (iEPSs) were comparatively evaluated. In vitro and in vivo antioxidant potentials were determined by using different methods and through different antioxidant enzymes. The antitumor activity was checked against a human colon cancer cell line. Analyses of the complete genome sequence were carried out to identify genes implicated in the production of nEPS. Thus, the genomic biosynthesis pathway and the export mechanism of nEPS were proposed. Analyses of irradiation data showed that iEPSs acquired new functional groups, lower molecular weights, and gained significantly (p < 0.05) higher antioxidant and antitumor abilities compared with nEPS. These findings provide a basis for using iEPSs as novel pharmaceutical agents for human therapies.

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Section: Antitumor Activities Of Neps and Iepsscontrasting

confidence: 78%

Effect of Gamma Irradiation on Enhanced Biological Activities of Exopolysaccharide from Halomonas desertis G11: Biochemical and Genomic Insights

Chouchane¹,

Boutiti²,

Ouertani³

et al. 2021

Polymers

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“…LPEPS was isolated as previously described [ 18 ]. Briefly, L. plantarum -12 was cultured in MRS medium at 37 °C for 24 h. The cell-free supernatant was achieved by centrifugation (10,000× g for 10 min at 4 °C).…”

Section: Methodsmentioning

confidence: 99%

“…In our previous studies, LPEPS was found to exert anti-proliferative effect on the human colon cancer cell line HT-29 in vitro [ 18 ]. In addition, LPEPS consisted of galactose, mannose, glucuronic acid, galactosamine, glucose, and xylose [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Exopolysaccharide Produced by Lactiplantibacillus plantarum-12 Alleviates Intestinal Inflammation and Colon Cancer Symptoms by Modulating the Gut Microbiome and Metabolites of C57BL/6 Mice Treated by Azoxymethane/Dextran Sulfate Sodium Salt

Song

Sun

et al. 2021

Foods

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Exopolysaccharide produced by Lactiplantibacillus plantarum-12 (LPEPS) exhibited the anti-proliferating effect on human colon cancer cell line HT-29 in vitro. The purpose of the study was to determine the alleviating effects of LPEPS on colon cancer development of the C57BL/6 mice treated by azoxymethane/dextran sulfate sodium salt (AOM/DSS). The C57BL/6 mice treated by AOM/DSS were orally administered LPEPS daily for 85 days. The results showed that LPEPS oral administration enhanced colon tight-junction protein expression and ameliorated colon shortening and tumor burden of the AOM/DSS treated mice. Furthermore, LPEPS oral administration significantly reduced pro-inflammatory factors TNF-α, IL-8, and IL-1β levels and increased anti-inflammatory factor IL-10 level in the serum of the AOM/DSS-treated mice. LPEPS oral administration reversed the alterations of gut flora in AOM/DSS-treated mice, as evidenced by the increasing of the abundance of Bacteroidetes, Bacteroidetes/Firmicutes ratio, Muribaculaceae, Burkholderiaceae, and norank_o__Rhodospirillales and the decreasing of the abundance of Firmicutes, Desulfovibrionaceae, Erysipelotrichaceae, and Helicobacteraceae. The fecal metabolites of the AOM/DSS-treated mice were altered by LPEPS oral administration, involving lipid metabolism and amino acid metabolism. Together, these results suggested that LPEPS oral administration alleviated AOM/DSS-induced colon cancer symptoms of the C57BL/6 mice by modulating gut microbiota and metabolites, enhancing intestine barrier, inhibiting NF-κB pathway, and activating caspase cascade.

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“…A similar study showed that crude exopolysaccharide produced by Lactobacillus plantarum-12 showed an antiproliferative effect on HT-29 cells, which is marked by the expression of proliferating cell nuclear antigen (PCNA). The pro-apoptotic effect was marked by the increased expression of apoptotic proteins such as Bax, Cytochrome C, caspase-3, -8, and -9 enzymes and decreased expression of the antiapoptotic protein Bcl-2 in the HT-29 cells [35].…”

Section: Restricting the Growth Of Cancerous Cellsmentioning

confidence: 99%

Probiotics: A Promising Candidate for Management of Colorectal Cancer

Tripathy

Dash

Kancharla³

et al. 2021

Cancers

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Colorectal cancer (CRC) is the World’s third most frequently diagnosed cancer type. It accounted for about 9.4% mortality out of the total incidences of cancer in the year 2020. According to estimated facts by World Health Organization (WHO), by 2030, 27 million new CRC cases, 17 million deaths, and around 75 million people living with the disease will appear. The facts and evidence that establish a link between the intestinal microflora and the occurrence of CRC are quite intuitive. Current shortcomings of chemo- and radiotherapies and the unavailability of appropriate treatment strategies for CRC are becoming the driving force to search for an alternative approach for the prevention, therapy, and management of CRC. Probiotics have been used for a long time due to their beneficial health effects, and now, it has become a popular candidate for the preventive and therapeutic treatment of CRC. The probiotics adopt different strategies such as the improvement of the intestinal barrier function, balancing of natural gut microflora, secretion of anticancer compounds, and degradation of carcinogenic compounds, which are useful in the prophylactic treatment of CRC. The pro-apoptotic ability of probiotics against cancerous cells makes them a potential therapeutic candidate against cancer diseases. Moreover, the immunomodulatory properties of probiotics have created interest among researchers to explore the therapeutic strategy by activating the immune system against cancerous cells. The present review discusses in detail different strategies and mechanisms of probiotics towards the prevention and treatment of CRC.

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The Effects of Lactobacillus plantarum-12 Crude Exopolysaccharides on the Cell Proliferation and Apoptosis of Human Colon Cancer (HT-29) Cells

Cited by 38 publications

References 45 publications

Effect of Gamma Irradiation on Enhanced Biological Activities of Exopolysaccharide from Halomonas desertis G11: Biochemical and Genomic Insights

Effect of Gamma Irradiation on Enhanced Biological Activities of Exopolysaccharide from Halomonas desertis G11: Biochemical and Genomic Insights

Exopolysaccharide Produced by Lactiplantibacillus plantarum-12 Alleviates Intestinal Inflammation and Colon Cancer Symptoms by Modulating the Gut Microbiome and Metabolites of C57BL/6 Mice Treated by Azoxymethane/Dextran Sulfate Sodium Salt

Probiotics: A Promising Candidate for Management of Colorectal Cancer

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