The Effects of Lanthanoid on the Structure–Function of Lactate Dehydrogenase from Mice Heart

Li, Na; Duan, Yanmei; Zhou, Min; Liu, Chao; Hong, Fashui

doi:10.1007/s12011-009-8374-1

Cited by 6 publications

(1 citation statement)

References 19 publications

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“…In recent years, microcalorimetric method has been used in biochemistry, biophysics, and medicine science, thus drawing increasing attention [16][17][18]. Furthermore, application of the spectroscopic techniques can reveal the reactivity of chemical and biological systems in low concentration under physiological conditions, and there have been several studies on fluorescence quenching and structure analysis of albumin induced by drugs or other bioactive small molecules [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Biological Activation of Heteropoly Complex of Molybdotungstosilicate Containing Lanthanum K10H3La(SiMo6W5O39)2⋅26H2O: Spectroscopic Approach and Microcalorimetry

Ouyang

Zhao

et al. 2009

Biol Trace Elem Res

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In this paper, the biological activation of heteropoly complex of molybdotungstosilicate containing lanthanum K(10)H(3)La(SiMo(6)W(5)O(39))(2)x26H(2)O (LaW(5)) was investigated by spectroscopic approach and microcalorimetry under the human physiological conditions. Fluorescence spectroscopy in combination with UV-Vis absorption spectroscopy was employed to investigate the binding of LaW(5) to bovine serum albumin (BSA). In the mechanism discussion, it was proved that the fluorescence quenching of BSA by LaW(5) is a result of the formation of LaW(5)-BSA complex. Binding parameters were determined using the Stern-Volmer equation. The results of thermodynamic parameters G, H, S at different temperatures indicate that van der Waals interactions and hydrogen bonds play a major role for LaW(5)-BSA association. The distance r between donor (BSA) and acceptor (LaW(5)) was obtained according to fluorescence resonance energy transfer. Furthermore, the calorimetric method was used to monitor the biological activity of LaW(5) in Escherichia coli.

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Section: Introductionmentioning

confidence: 99%

Biological Activation of Heteropoly Complex of Molybdotungstosilicate Containing Lanthanum K10H3La(SiMo6W5O39)2⋅26H2O: Spectroscopic Approach and Microcalorimetry

Ouyang

Zhao

et al. 2009

Biol Trace Elem Res

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Lanthanum chloride reduces lactate production in primary culture rat cortical astrocytes and suppresses primary co-culture rat cortical astrocyte-neuron lactate transport

Sun

Yang

et al. 2017

Arch Toxicol

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Lanthanum (La) can impair learning memory and induce behavioral abnormalities in animals. However, the mechanism underlying these adverse effects of La is still elusive. It has been demonstrated that lactate derived from astrocytes is the major energy source for neurons during long-term memory (LTM) formation and the deficiency of lactate supply can result in LTM damage. However, little work has been done with respect to the impact of La on the lactate production in astrocytes and astrocyte-neuron lactate transport (ANLT). Herein, experiments were undertaken to explore if there was such an adverse effect of La. Primary culture rat cortical astrocytes and primary co-culture rat cortical astrocyte-neuron were treated with (0.125, 0.25 and 0.5 mM) lanthanum chloride (LaCl) for 24 h. The results showed that LaCl treatment significantly downregulated the mRNA and protein expression of glucose transporter 1 (GLUT1), glycogen synthase (GS), glycogen phosphorylase (GP), lactate dehydrogenase A (LDHA), and monocarboxylate transporter 1, 2 and 4 (MCT 1 2 and 4); upregulated the mRNA and protein expression of lactate dehydrogenase B (LDHB); and decreased the glycogen level, total LDH and GP activity, GS/p-GS ratio and lactate contents. Moreover, rolipram (20, 40 μM) or forskolin (20, 40 μM) could increase the lactate content by upregulating GP expression and the GS/p-GS ratio, as well as antagonize the effects of La. These results suggested that La-induced learning-memory damage was probably related to its suppression of lactate production in astrocytes and ANLT. This study provides some novel clues for clarifying the mechanism underlying the neurotoxicity of La.

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Lanthanum damages learning and memory and suppresses astrocyte–neuron lactate shuttle in rat hippocampus

Jin

Gao

et al. 2017

Exp Brain Res

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Rare-earth elements (REEs) are applied in various fields by virtue of their superior physical and chemical properties. Surveys have reported that REEs can impair learning and memory in children and induce neurobehavioral abnormalities in animals. However, the mechanism underlying this neurotoxicity is still unclear. Lanthanum (La) is often chosen to study the effects of REEs. Here, we investigated the role of astrocyte-neuron lactate shuttle (ANLS) in spatial learning and memory impairment induced by LaCl in hippocampus, an important spatial memory-related brain region. Pregnant Wistar rats were exposed to 0, 0.125, 0.25, 0.5, or 1% LaCl in drinking water during pregnancy and lactation. After weaning, young rats continued to receive 0, 0.125, 0.25, 0.5, and 1% LaCl in the drinking water for 1 month. The results showed that LaCl exposure impaired the spatial learning and memory of rats in Morris water maze test, significantly reduced the mRNA and protein levels of glycogen synthetase, glycogen phosphorylase, lactate dehydrogenase A, monocarboxylate transporter 4, MCT-1, and MCT-2, and decreased total LDH activity and lactate contents in rat hippocampus. These results indicate that LaCl impairs spatial learning and memory in rats probably by suppressing ANLS in rat hippocampus. The study provides a novel clue of energy supply for neurons to clarify the neurotoxicity of REEs.

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The Effects of Lanthanoid on the Structure–Function of Lactate Dehydrogenase from Mice Heart

Cited by 6 publications

References 19 publications

Biological Activation of Heteropoly Complex of Molybdotungstosilicate Containing Lanthanum K10H3La(SiMo6W5O39)2⋅26H2O: Spectroscopic Approach and Microcalorimetry

Biological Activation of Heteropoly Complex of Molybdotungstosilicate Containing Lanthanum K10H3La(SiMo6W5O39)2⋅26H2O: Spectroscopic Approach and Microcalorimetry

Lanthanum chloride reduces lactate production in primary culture rat cortical astrocytes and suppresses primary co-culture rat cortical astrocyte-neuron lactate transport

Lanthanum damages learning and memory and suppresses astrocyte–neuron lactate shuttle in rat hippocampus

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