The effects of lanthanum chloride on proliferation and apoptosis of cervical cancer cells: involvement of let-7a and miR-34a microRNAs

Yu, Lingfang; Xiong, Jing; Guo, Ling; Miao, Lifang; Liu, Sisun; Guo, Fei

doi:10.1007/s10534-015-9872-6

Cited by 35 publications

(24 citation statements)

References 50 publications

(52 reference statements)

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“…Alterations of this miRNA in our study were similar to those described in the literature earlier by our group as well as by other investigators [22,23,40–42]. The aberrant expression of let-7a in many types of human cancers including PC indicates its tumor suppressor role [22,24,43,44]. For example, over-expression of let-7a with diflourinated-curcumin (CDF) mediated tumor regression with reduced EZH2, Notch-1, and EpCAM expression in PC in our previous study [22].…”

Section: Discussionsupporting

confidence: 90%

Differential Expression of Micrornas in Tissues and Plasma Co-exists as a Biomarker for Pancreatic Cancer

et al. 2015

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Objective Pancreatic cancer (PC) is a lethal disease with disappointing results from current treatment modalities, suggesting that novel therapeutic strategies are urgently needed. Since microRNAs (miRNAs) are important player in biology, the clinical utility of miRNAs for designing novel therapeutics is an active area of research. The objective of the present study was to examine differentially expressed miRNAs between normal and tumor tissues, and in plasma samples obtained from PC patients, chronic pancreatitis (CP) patients and healthy subjects (HC). Material and methods The miRNA expression profiling using formalin-fixed paraffin embedded (FFPE) tissues from normal and tumor specimens was accomplished using miRBase version 19 (LC Sciences, Houston, TX, USA). Quantitative real-time PCR (qRT-PCR) was subsequently performed in individual samples for 7 selected miRNAs. In addition, qRT-PCR was also performed for assessing the expression of 8 selected miRNAs in plasma samples. Results A significant difference in the expressions of miR-21, miR-205, miR-155, miR-31, miR-203, miR-214 and miR-129-2 were found in tumor tissue samples. Lower expression of miR-214 was found to be associated with better overall survival. We also observed differential expression of 8 miRNAs in plasma samples of CP and PC patients compared to HC. Interestingly, over expression of miR-21, and miR-31 was noted in both tumor tissues and in the plasma. Conclusion We found deregulated expression of miRNAs that could distinguish normal from PC in two different types of samples (tissues and plasma). Interestingly, lower expression of miR-214 was found to be associated with better overall survival. Although not statistically significant, we also observed higher expression of let-7a and lower expression of miR-508 to be associated with overall better survival. We conclude that our study nicely lays the foundation for detailed future investigations for assessing the role of these miRNAs in the pathology of pancreatic cancer.

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Section: Discussionsupporting

confidence: 90%

Differential Expression of Micrornas in Tissues and Plasma Co-exists as a Biomarker for Pancreatic Cancer

et al. 2015

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“…| 17089 several tumor types, including cervical cancer (Fujita et al, 2008;Wang, Yang, Li, & Han, 2015;Yu et al, 2015).…”

Section: The Role Of Micrornas In the Chemoresistance Of Cervical Cmentioning

confidence: 99%

microRNAs: New prognostic, diagnostic, and therapeutic biomarkers in cervical cancer

Nahand

Taghizadeh-Boroujeni

Karimzadeh

et al. 2019

Journal Cellular Physiology

171

100

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Cervical cancer is as a kind of cancer beginning from the cervix. Given that cervical cancer could be observed in women who infected with papillomavirus, regular oral contraceptives, and multiple pregnancies. Early detection of cervical cancer is one of the most important aspects of the therapy of this malignancy. Despite several efforts, finding and developing new biomarkers for cervical cancer diagnosis are required. Among various prognostic, diagnostic, and therapeutic biomarkers, miRNA have been emerged as powerful biomarkers for detection, treatment, and monitoring of response to therapy in cervical cancer. Here, we summarized various miRNAs as an employable platform for prognostic, diagnostic, and therapeutic biomarkers in the treatment of cervical cancer.

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“…Let-7a is also involved in many important signaling pathways in cancer progression. 17,18 Furthermore, let-7a induces apoptosis in a number of cancers including gastric carcinoma, 19 nasopharyngeal carcinoma, 20 hepatocellular cancer, 21 breast cancer, 22 cervical cancer, 23 brain cancer, 24 and prostate cancer. 25 For melanoma, let-7a was found to reduce invasive potential of melanoma cells by regulating the expression of integrin-β3.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-26a inhibits the growth and invasiveness of malignant melanoma and directly targets on MITF gene

2017

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Metastatic melanoma is the most aggressive form of skin cancer and is refractory to therapy. MicroRNAs have been recently discovered as novel molecules that provide therapeutic benefits against melanoma. This work aims to examine the effects of miR-26a and let-7a on the growth and invasiveness of malignant melanoma in vitro and in vivo. In addition, we elucidate the mechanism of action by identifying the target gene of miR-26a. Both miR-26a and let-7a inhibited proliferation and invasiveness and halted the cell cycle at the G 1 /G 0 phase in SKMEL-28 and WM1552C malignant melanoma cell lines. Moreover, miR-26a potently induced apoptosis and downregulated the expressions of microphthalmia-associated transcription factor (MITF) and MAP4K3 in both cell lines. The luciferase reporter assay demonstrated that miR-26a suppresses MITF expression by binding the 3′-UTR, suggesting that MITF is a bona fide target of miR-26a. SiRNA knockdown of the MITF gene confirmed that miR-26a reduced cell viability and induced apoptosis by regulating MITF. Using a murine model, we also found miR-26a significantly retarded the growth of melanoma tumors in vivo. In conclusion, miR-26a and let-7a suppressed the growth and invasiveness of melanoma cells, suggesting that miR-26a and let-7a may represent novel therapies for malignant melanoma.

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The effects of lanthanum chloride on proliferation and apoptosis of cervical cancer cells: involvement of let-7a and miR-34a microRNAs

Cited by 35 publications

References 50 publications

Differential Expression of Micrornas in Tissues and Plasma Co-exists as a Biomarker for Pancreatic Cancer

Differential Expression of Micrornas in Tissues and Plasma Co-exists as a Biomarker for Pancreatic Cancer

microRNAs: New prognostic, diagnostic, and therapeutic biomarkers in cervical cancer

MicroRNA-26a inhibits the growth and invasiveness of malignant melanoma and directly targets on MITF gene

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