The effects of long-term treatment with eicosapentaenoic acid and docosahexaenoic acid on hypoxia/ rexoygenation injury of isolated cardiac cells in adult rats

Hayashi, Michihiko; Nasa, Yoshihisa; Tanonaka, Kouichi; Sasaki, Hideo; Miyake, Rikako; Hayashi, Jyun-ichi; Takeo, Satoshi

doi:10.1016/0022-2828(95)90024-1

Cited by 25 publications

(12 citation statements)

References 41 publications

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“…DHA inhibits activation of this transcription factor in other systems (27,47). Furthermore, this inhibitory effect on TNF-␣ may also help explain the beneficial effects of long-term DHA-containing diets on acute ischemic injury to various tissues (24,48,49). Although TNF-␣ production is also controlled at the translational step (50), decreasing mRNA abundance should ultimately inhibit production of this molecule.…”

Section: Discussionmentioning

confidence: 99%

Docosahexaenoic Acid Ameliorates Murine Ischemic Acute Renal Failure and Prevents Increases in mRNA Abundance for both TNF-α and Inducible Nitric Oxide Synthase

Kielar¹,

Jeyarajah²,

Zhou³

et al. 2003

Journal of the American Society of Nephrology

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Abstract. This study demonstrates that intraperitoneal injections of DHA (all cis 4,7,10,13,16,19 docosahexaenoic acid C22: n-3) bound to bovine serum albumin ameliorate murine acute renal failure (ARF) induced by temporary occlusion of the renal artery. Three micromoles of DHA decreased serum creatinine (Scr) from 2.3 mg/dl to 1.1 mg/dl 24 h after reperfusion (n ϭ 15; P Ͻ 0.05). Scr of the treated animals were significantly lower than controls throughout a 7-d time course. Although lower doses of DHA were less effective, higher doses were not more effective. Ribonuclease (RNase) protection assays showed that ischemia increased mRNA abundance for TNF-␣ and inducible nitric oxide synthase (iNOS) at 24 h. This increase was prevented by DHA administration. Because TNF-␣ and iNOS contribute to renal ischemic injury, their inhibition may contribute to DHA's salutary effect. In addition, the data may have therapeutic implications, because the DHA improves ARF even when administered at 4 h after reperfusion.

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Section: Discussionmentioning

confidence: 99%

Docosahexaenoic Acid Ameliorates Murine Ischemic Acute Renal Failure and Prevents Increases in mRNA Abundance for both TNF-α and Inducible Nitric Oxide Synthase

Kielar¹,

Jeyarajah²,

Zhou³

et al. 2003

Journal of the American Society of Nephrology

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“…Exogenous DHA may even be deleterious during ischemia and reperfusion [181]. The majority of studies agreed that EPA is responsible for the beneficial effects of n-3 PUFAs when compared with DHA [91, 179,181,182], although some investigations also showed a protective effect of DHA [178,183]. The higher the level of EPA in cardiac phospholipids, the greater the protection during ischemia and reperfusion [91].…”

Section: Cell Damage During Ischemia and Reperfusionmentioning

confidence: 99%

“…Although some studies were inconclusive [162 -164], the vast majority reported a beneficial effect of either marine or plant n-3 PUFAs. Long-term dietary enrichment with n-3 PUFAs reduced infarct size [165 -167], improved the recovery of mechanical cardiac activity during the reestablishment of coronary flow [90, 91, 168 -173], prevented the release of creatine kinase or lactate dehydrogenase [90, 91, 169 -171, 174, 175], favoured the recovery of action potential during reoxygenation [176,177] and improved cell survival during hypoxia [178,179]. These studies thus clearly indicated that n-3 PUFAs reduce the cellular damage triggered by ischemia and reperfusion.…”

Section: Cell Damage During Ischemia and Reperfusionmentioning

confidence: 99%

Dietary n-3 polyunsaturated fatty acids and coronary heart disease-related mortality: a possible mechanism of action

Demaison¹,

Moreau²

2002

Cellular and Molecular Life Sciences (CMLS)

106

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Epidemiological and interventional studies indicate that dietary n-3 PUFA reduces mortality due to coronary heart disease (CHD). They act at a low dose, since one or two meals with fatty fish per week is sufficient to provide protection when compared with no fish intake. These fatty acids are effective in providing primary prevention in low- and high-risk subjects and secondary prevention. At high doses, dietary n-3 PUFAs have several beneficial properties. First, they act favourably on blood characteristics: they are hypocholesterolemic and hypotriglyceridemic; they reduce platelet aggregation; they exhibit antithrombotic and fibrinolytic activities; they reduce blood viscosity and they exhibit antiinflammatory action. Second, they reduce ischemia/reperfusion-induced cellular damage. This effect is apparently due to the incorporation of eicosapentaenoic acid in membrane phospholipids. Third, they reduce ischemia and reperfusion arrhythmias. All the effects exerted by n-3 PUFAs at high doses are incompatible with the beneficial action on CHD mortality in humans observed at low doses, where their main properties are related to circulation in the form of free fatty acids. Numerous experimental studies have indicated that low concentrations of exogenous n-3 PUFAs reduce the severity of cardiac arrhythmias. This effect is probably responsible for the protective action of n-3 PUFA on CHD mortality. Further studies are necessary to confirm this assumption in animals. Such studies should take account of the fact that only a low dose of n-3 PUFA (20 mg/kg/day) is necessary to afford protection. Furthermore, since the beneficial effect of n-3 PUFAs on CHD mortality is observed in fish eaters versus no-fish eaters, and since populations in industrialised countries consume excess n-6 PUFAs, control animals in long-term dietary experiments should be fed a diet with only n-6 fatty acids as a source of PUFAs.

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“…Las células individuales se clasificaron en uno de los siguientes tres grupos fenotípicos: (1) Células en forma de bastón con clara forma alargada con estriado de sarcómeras definidas, membrana celular lisa y regular, que excluían el Tripán azul; (2) Células de forma redonda sin estrías claras y contractura aparente, que incluían el azul tripán, y (3) Células de forma rectangular, con estrías y superficie regular, que excluyeron el azul tripán. Estas distinciones fueron hechas porque los cardiomiocitos maduros típicos poseen un sarcolema intacto, tienen forma de bastón y son de aspecto rectangular, y se consideran viables, mientras que cuando los cardiomiocitos se dañan, se acortan y luego se hiper-contraen, dando la apariencia de células en forma redondeada y no se consideran viables (Hayashi, 1995, Gomez, 1997.…”

Section: Morfología Y Exclusión Del Tripán Azulunclassified

Preacondicionamiento isquémico en cardiomiocitos ventriculares aislados. Identificación y expresión de algunos microRNAs asociados

Gómez-Grosso

2014

Rev. Acad. Colomb. Cienc. Ex. Fis. Nat.

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RESUMENGómez-Grosso L.A.: Preacondicionamiento isquémico en cardiomiocitos ventriculares aislados. Identificación y expresión de algunos microRNAs asociados. Rev. Acad. Colomb. Cienc. 37 (145): [433][434][435][436][437][438][439][440][441][442][443][444][445][446][447] 2013. ISSN 0370-3908.El objetivo de este estudio fue evaluar el preacondicionamiento isquémico, PCI, en cardiomiocitos ventriculares de cobayo adultos individuales y evaluar el perfil de expresión diferencial de microRNAs en estas células. Una supervisión de la morfología, de la actividad metabólica de las células cardíacas individuales aisladas en tiempo real, permite una evaluación de los cambios en la viabilidad de los cardiomiocitos y proporciona evidencia experimental de que el PCI tiene acción citoprotectora directa mediante la promoción de la supervivencia in vitro. La protección cardiaca por PCI asociada con la expresión diferencial de microRNAs específicos en cardiomicitos adultos aislados sugiere que algunos miRNAs están involucrados en lesión y cardioprotección.Palabras clave: cardioprotección, preacondicionamiento, ensayo de MTT, tiempo real, cardiomiocitos, viabilidad, isquemia, Dinitrofenol, Adenosina. ABSTRACTThe aim of this study was to evaluate the ischemic preconditioning, PCI, in single quiescent adult guinea-pig ventricular myocytes and assess the differential expression of microRNAs in these cells. Monitoring of the morphology of the metabolic activity of individual cardiac cells isolated in real time, allows evaluation of changes in the viability of the cardiomyocytes and provides experimental evidence that PCI has direct cytoprotective action by promoting survival in vitro. PCI cardiac protection associated with the differential expression of specific microRNAs in isolated adult cardiomyocytes suggests that miRNAs are involved in some injury and cardioprotection.

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The effects of long-term treatment with eicosapentaenoic acid and docosahexaenoic acid on hypoxia/ rexoygenation injury of isolated cardiac cells in adult rats

Cited by 25 publications

References 41 publications

Docosahexaenoic Acid Ameliorates Murine Ischemic Acute Renal Failure and Prevents Increases in mRNA Abundance for both TNF-α and Inducible Nitric Oxide Synthase

Docosahexaenoic Acid Ameliorates Murine Ischemic Acute Renal Failure and Prevents Increases in mRNA Abundance for both TNF-α and Inducible Nitric Oxide Synthase

Dietary n-3 polyunsaturated fatty acids and coronary heart disease-related mortality: a possible mechanism of action

Preacondicionamiento isquémico en cardiomiocitos ventriculares aislados. Identificación y expresión de algunos microRNAs asociados

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