The effects of low level laser therapy on injured skeletal muscle

Rennó, Ana Cláudia Muniz; Assis, Lívia; Peres, Beatriz; Rodrigues, Natália Camargo; Brunelli, Roberta de Matos; Toma, Renata Luri; Medalha, Carla Christina; Ribeiro, Daniel Araki

doi:10.1590/s1516-89132014000100008

Cited by 7 publications

(4 citation statements)

References 25 publications

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“…Both Roth et al and Weiss et al demonstrated that injured areas of muscle were filled with new myofibrils capable of contractile activity [28, 37]; additionally, Shefer et al showed that laser irradiation could stimulate the satellite cells near myofibrils and form new myofibrils for muscular repair [27]. Moreover, laser irradiation could modulate the collagen content in injured areas through nuclear factor kappa-B (NF-kB) gene expression, collagen I and III remodeling [38], and a reduction in collagen content in skeletal muscles [39]. Rodriguez et al indicated that the hADSCs could repair a posterior tibialis muscle injury via formation and fusion of new myotubes [40].…”

Section: Discussionmentioning

confidence: 99%

Combination of laser and human adipose-derived stem cells in repair of rabbit anal sphincter injury: a new therapeutic approach

et al. 2019

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BackgroundAnal sphincter injury leads to fecal incontinence. Based on the regenerative capability of laser and human adipose-derived stem cells (hADSCs), this study was designed to assess the effects of co-application of these therapies on anal sphincter recovery after injury.DesignMale rabbits were assigned to equal groups (n = 7) including control, sphincterotomy, sphincterotomy treated with laser (660 nm, 90 s, immediately after sphincterotomy, daily, 14 days), hADSCs (2 × 106 hADSCs injected into injured area of the sphincter immediately after sphincterotomy), and laser + hADSCs. Ninety days after sphincterotomy, manometry and electromyography were performed, sphincter collagen content was evaluated, and Ki67, myosin heavy chain (MHC), skeletal muscle alpha-actin (ACTA1), vascular endothelial growth factor A (VEGFA), and vimentin mRNA gene expression were assessed.ResultsThe laser + hADSCs group had a higher resting pressure compared with the sphincterotomy (p < 0.0001), laser (p < 0.0001), and hADSCs (p = 0.04) groups. Maximum squeeze pressure was improved in all treated animals compared with the sphincterotomized animals (p < 0.0001), without a significant difference between treatments (p > 0.05). In the laser + hADSCs group, motor unit numbers were higher than those in the laser group (p < 0.0001) but did not differ from the hADSCs group (p = 0.075). Sphincterotomy increased collagen content, but the muscle content (p = 0.36) and collagen content (p = 0.37) were not significantly different between the laser + hADSCs and control groups. Laser + hADSCs increased ACTA1 (p = 0.001) and MHC (p < 0.0001) gene expression compared with laser or hADSCs alone and was associated with increased VEGFA (p = 0.009) and Ki67 mRNA expression (p = 0.01) and decreased vimentin mRNA expression (p < 0.0001) compared with laser.ConclusionThe combination of laser and hADSCs appears more effective than either treatment alone for promoting myogenesis, angiogenesis, and functional recovery after anal sphincterotomy.

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Section: Discussionmentioning

confidence: 99%

Combination of laser and human adipose-derived stem cells in repair of rabbit anal sphincter injury: a new therapeutic approach

et al. 2019

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“…The laser does this by stimulating the satellite cells adjacent to the adult myofibrils [ 19 ]. In addition to increasing myofibrils, laser by modifying the Nuclear Factor Kappa B (NF-κB) expression causes collagen remodeling [ 36 ] and reduces collagen levels [ 37 ], thereby advancing the muscle repair process. In the present study, the amount of collagen in the laser group also confirms the laser's regulatory role on collagen during the repair process.…”

Section: Discussionmentioning

confidence: 99%

Combined application of chondroitinase ABC and photobiomodulation with low-intensity laser on the anal sphincter repair in rabbit

et al. 2021

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Background Photobiomodulation with low-intensity laser (LIL) and chondroitinase ABC (ChABC) can repair damaged muscle tissue, so the aim of this study was to investigate the effect of co-administration of these two factors on anal sphincter repair in rabbits. Methods Male rabbits were studied in 5 groups (n = 7): Control (intact), sphincterotomy, laser, ChABC and laser + ChABC. 90 days after intervention were evaluated resting and maximum squeeze pressures, number of motor units, collagen amount, markers of muscle regeneration and angiogenesis. Results Resting pressure in the Laser + ChABC group was higher than the sphincterotomy, laser and ChABC groups (p < 0.0001). Maximum squeeze pressure in the all study groups was higher than sphincterotomy group (p < 0.0001). In the laser + ChABC and ChABC groups, motor unit numbers were more than the sphincterotomy group (p < 0.0001). Collagen content was significantly decreased in the laser (p < 0.0001) and laser + ChABC groups. ACTA1 (p = 0.001) and MHC (p < 0.0001) gene expression in the Laser + ChABC group were more than the laser or ChABC alone. VEGFA (p = 0.009) and Ki67 mRNA expression (p = 0.01) in the Laser + ChABC group were more than the laser group, But vimentin mRNA expression (p < 0.0001) was less than the laser group. Conclusion Co-administration of ChABCs and photobiomodulation with LIL appears to improve the tissue structure and function of the anal sphincter in rabbits more than when used alone.

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“…LLLT is used to speed up the process of regeneration as noted by Renno et al 17 , who evaluated rats submitted to cryolesion and treated with AsGaAl laser (808 nm, 50 mw, and 10 J/cm 2 and 50 J/cm 2 energy densities) and found reduction in the areas of cellular infiltrate and injury compared to the control group after 13 days. A similar result was found after 21 days of cryolesion in the control and malnourished groups in this work.…”

Section: Discussionmentioning

confidence: 99%

Laser de baixa intensidade favorece a regeneração muscular em modelo experimental desnutrido e recuperado

Silva

Silveira

Matsumura³

et al. 2018

Fisioter. Pesqui.

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RESUMO A terapia por laser de baixa intensidade (Low-Level Laser Therapy - LLLT) é utilizada com frequência nas lesões musculares, mas precisa ser investigada em modelo de desnutrição. O objetivo desse estudo foi analisar os efeitos da LLLT na regeneração muscular de ratos submetidos à desnutrição e recuperação proteica. Foram utilizados 40 ratos Wistar, recém-desmamados, divididos em grupo controle (C), que consumiu ração normoproteica (14% caseína), e grupo desnutrido (D), que consumiu ração hipoproteica (6% caseína) por 45 dias e ração normoproteica até o final do experimento. Posteriormente, o músculo tibial anterior direito foi criolesado e tratado com LLLT (AsGaAl 830nm, 30mW, 20J/cm²), três vezes por semana, por 7 e 21 dias. Houve redução da área de inflamação/regeneração no grupo C21 comparado ao D21 (p<0,05), sendo mais evidente com a LLLT (C21L e D21L). O conteúdo de TNF-α foi reduzido após 21 dias da lesão. A área de densidade de tecido conjuntivo (ADTC) foi menor nos grupos C21 e C21L comparados aos respectivos grupos desnutridos (p<0,05). A LLLT reduziu a ADTC no grupo D21L quando comparado do D21 (p<0,05), porém o conteúdo de TGF-β1 não foi influenciado. A área de secção transversa (AST) da fibra muscular aumentou nos grupos 21 dias. A m-TOR apresentou maior conteúdo no grupo C21L quando comparado ao D21L (p<0,05). Concluiu-se que a LLLT favoreceu a regeneração muscular na fase tardia no modelo experimental de desnutrição pós-natal e posterior recuperação proteica.

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The effects of low level laser therapy on injured skeletal muscle

Abstract: The main purpose of the present study was to investigate the effects of low-level laser therapy (LLLT)

Cited by 7 publications

References 25 publications

Combination of laser and human adipose-derived stem cells in repair of rabbit anal sphincter injury: a new therapeutic approach

Combination of laser and human adipose-derived stem cells in repair of rabbit anal sphincter injury: a new therapeutic approach

Combined application of chondroitinase ABC and photobiomodulation with low-intensity laser on the anal sphincter repair in rabbit

Laser de baixa intensidade favorece a regeneração muscular em modelo experimental desnutrido e recuperado

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