To study the variation of environment in the professional classroom during lecture hours, multiple field experiments and intervention experiments on indoor and outdoor temperatures were conducted in a university professional classroom in Shaoxing during the spring. Environmental data, including indoor and outdoor temperatures, relative, and CO2 concentrations, were recorded every 5 min. Volatile organic compounds (VOC) were sampled, and indoor air quality was evaluated repeatedly. Results showed that the classroom’s average indoor air temperature ranged from 17.8–29.2 ℃, the average indoor relative humidity from 34.5%–91.0%, the average CO2 concentrations from 921.6–1805.2 ppmv, and total VOC concentrations from 330–682 ppbm. The subjective evaluation conducted during the intervention experiments indicated a significant increase in perceived odor intensity upon entering the classroom. When the CO2 concentration reached 2000 ppmv, the satisfaction and acceptability of the air quality for the subjects and invitees decreased significantly. In the temperature range of 17–31 ℃, the CO2 emission rate of human body was estimated to increase by 0.78 L/h for every 1 ℃ increase in temperature. To maintain the indoor CO2 concentration at 1000 ppmv, the required ventilation rate for each person must be increased by 0.25 ± 0.3 L/s.