The effects of mebendazole on the growth and development of <i>Caenorhabditis elegans</i>

Spence, Andrew M.; Malone, Kathleen M. B.; Novak, Marie; Woods, Robin A.

doi:10.1139/z82-336

Cited by 16 publications

(11 citation statements)

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“…All development. Each of the larval moults occurred within the time range prevously reported by our laboratory (Spence et al 1982). Reproductive development was similarly unaffected; vulvae and eggs could be discerned in treated worms at the same time as in the controls.…”

Section: Drug Concn (Mm)supporting

confidence: 65%

“…The media and growth conditions for plate cultures were as described previously (Spence et al 1982). Stock solutions of ADT and dADT for addition to agar media were prepared at 5 m g .…”

Section: Media and Growth Conditionsmentioning

confidence: 99%

“…Worms were transferred to microscope slides in a drop of water and measured using a Leitz projection microscope at a total magnification of 1 OOX (Spence et al 1982).…”

Section: Growth Measurementsmentioning

confidence: 99%

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The effects of amidantel (BAY d 8815) and its deacylated derivative (BAY d 9216) on wild-type and resistant mutants of Caenorhabditis elegans

Woods¹,

Malone²,

Albuquerque³

et al. 1986

Can. J. Zool.

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1986. The effects of amidantel (BAY d 88 15) and its deacylated derivative (BAY d 9216) on wild-type and resistant mutants of Caenorhabditis elegans. Can. J . Zool. 64: 1310-1316. The anthelmintic drugs amidantel (BAY d 88 15) and its deacylated derivative (BAY d 9216) inhibited the growth of wild-type (N2) Caenorhabditis elegans but had little effect on development or reproductive capacity. Inhibition of growth correlated well with drug-induced paralysis, both becoming maximal at around 1 .O mM concentration of either drug. Egg laying was delayed by about 24 h and the rate of laying was only about 60-70% of the controls. However, the period during which eggs were laid was extended by a similar amount and the total number of eggs laid was the same for controls and drug-treated worms. Five drug-resistant mutants (TI 14, T22, T26, T21 6, and T226) were isolated following ethylmethanesulphonate mutagenesis. All were shorter than N2 at 96 h on drug-free medium; their growth was not further impaired by either of the anthelmintic drugs. All except T I 14 exhibited a normal pattern of sexual maturation. Cultures of T I 14 at 96 h contained many immature worms. This mutant also exhibited the most impaired motility, being severely uncoordinated in liquid suspension. The other mutants could swim normally but were noticeably slower than N2. Genetic analysis indicated that each mutant was the result of a single genetic lesion, that the mutants were recessive, and that there were two genes for amidantel resistance (adtl and adt2). In vitro studies on representatives of each class (T114 and T22) indicated a defect in the acetylcholine receptor. T22 mutants showed a moderate decrease in sensitivity towards typical cholinergic agonists as well as the anthelmintic drugs, while T I 14 mutants were apparently devoid of functional pharmacological acetylcholine receptors. WOODS, R. A., K. M. B. MALONE, C. A. ALBUQUERQUE et G. TOMI~INSON. 1986. The effects of amidantel (BAY d 88 15) and its deacylated derivative (BAY d 9216) on wild-type and resistant mutants of Caenorhabditis elegans. Can. J . Zool. 64: 1310-1316. L'amidantel (BAY d 88 15), une substance anti-helminthes, ou son derive desacetyle (BAY d 9216) inhibent la croissance des Caenorhabditis elegans de type sauvage (N2), mais n'ont que peu d'effet sur le d~v e l o~~e m e n t ou sur la capacite reproductrice des vers. I1 y a une forte correlation entre l'inhibitation de la croissance et la paralysie qu'entralnent les substances et ces deux facteurs atteignent leur sommet lorsque la concentration de la substance atteint 1 ,O mM. La ponte est retardee d'environ 24 h et le taux de ponte n'atteint que 60-70% de la valeur enregistree chez les tCmoins. Cependant, la ponte dure plus longtemps chez les vers traites et le nombre total d'oeufs pondus est semblable a celui qui prevaut chez les temoins. Cinq mutants resistants aux produits (TI 14, T22, T26, T216 et T226) ont kt6 isolks apres une mutagenese causee par de l'ethyl-methane-sulfonate. Tous les mutants sont plus courts que N2 ...

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Section: Drug Concn (Mm)supporting

confidence: 65%

“…The media and growth conditions for plate cultures were as described previously (Spence et al 1982). Stock solutions of ADT and dADT for addition to agar media were prepared at 5 m g .…”

Section: Media and Growth Conditionsmentioning

confidence: 99%

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The effects of amidantel (BAY d 8815) and its deacylated derivative (BAY d 9216) on wild-type and resistant mutants of Caenorhabditis elegans

Woods¹,

Malone²,

Albuquerque³

et al. 1986

Can. J. Zool.

Self Cite

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“…Nematodes become paralysed by low doses of ABM and, thus, ABM was used as a comparison for acute toxicity (Martin et al, 2002). MBZ is an antihelmintic drug that inhibits the synthesis of microtubules in the intestine, blocking uptake of nutrients, causing a more gradual paralysis than ABM (Spence et al, 1982). Therefore, MBZ was used as a comparison for chronic effects.…”

Section: Staged Developmental Assays With C Elegansmentioning

confidence: 99%

Spirotetramat causes an arrest of nematode juvenile development

Vang

Opperman

Schwarz

et al. 2016

Nematol

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Spirotetramat (Movento™, Bayer CropScience) (SPT), an effective insecticide, has also demonstrated potential activity as a nematicide. No significant effects on hatching rates of Caenorhabditis elegans, Meloidogyne incognita or Heterodera glycines were observed when eggs were soaked in a maximum concentration of 105 ppm of technical grade spirotetramat-enol (SPT-enol), the active form in plants. Synchronised first-stage juveniles of C. elegans soaked in SPT-enol concentrations as low as 30 ppm demonstrated arrested juvenile development with calculated EC95 of 44-48 ppm. Single applications of formulated SPT (Movento 240SC) were applied to plant foliage at the labelled insecticidal rate of 87.6 g a.s. ha−1 at 1-week intervals on soybean plants inoculated with H. glycines or tomato plants inoculated with M. incognita in glasshouse tests. SPT consistently inhibited nematode development to reproductive maturity when applied at 1-2 weeks after inoculation. Optimal SPT application timings coincide with early stages of root infection, when nematodes are still in vulnerable juvenile stages.

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“…Three-animal sets were incubated on E. coli in 200-lL complete S medium containing one of the samples in 250-lL hemiellipsoidal wells at 20°C [3,11]. DMSO at concentrations up to 1% has no effect on the development of C. elegans [12]. In tests on 9, water-insoluble, the final concentration of DMSO was maintained at 0.8%.…”

Section: Generalmentioning

confidence: 99%

Potential anthelmintics: polyphenols from the tea plant Camellia sinensis L. are lethally toxic to Caenorhabditis elegans

et al. 2008

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A novel gallate of tannin, (-)-epigallocatechin-(2 beta-->O-->7',4 beta-->8')-epicatechin-3'-O-gallate (8), together with (-)-epicatechin-3-O-gallate (4), (-)-epigallocatechin (5), (-)-epigallocatechin-3-O-gallate (6), and (+)-gallocatechin-(4 alpha-->8')-epigallocatechin (7), were isolated from the tea plant Camellia sinensis (L.) O. Kuntze var. sinensis (cv., Yabukita). The structure of 8, including stereochemistry, was elucidated by spectroscopic methods and hydrolysis. The compounds, along with commercially available pyrogallol (1), (+)-catechin (2), and (-)-epicatechin (3), were examined for toxicity towards egg-bearing adults of Caenorhabditis elegans. The anthelmintic mebendazole (9) was used as a positive control. Neither 2 nor 3 were toxic but the other compounds were toxic in the descending order 8, 7 approximately 6, 9, 4, 5, 1. The LC(50) (96 h) values of 8 and 9 were evaluated as 49 and 334 micromol L(-1), respectively. These data show that many green tea polyphenols may be potential anthelmintics.

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The effects of mebendazole on the growth and development of Caenorhabditis elegans

Cited by 16 publications

References 0 publications

The effects of amidantel (BAY d 8815) and its deacylated derivative (BAY d 9216) on wild-type and resistant mutants of Caenorhabditis elegans

The effects of amidantel (BAY d 8815) and its deacylated derivative (BAY d 9216) on wild-type and resistant mutants of Caenorhabditis elegans

Spirotetramat causes an arrest of nematode juvenile development

Potential anthelmintics: polyphenols from the tea plant Camellia sinensis L. are lethally toxic to Caenorhabditis elegans

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