The effects of methyl jasmonate and salicylic acid on the production of secondary metabolites in organ culture of Ziziphora persica

Zare-Hassani, Elham; Motafakkerazad, Rouhollah; Razeghi, Jafar; Kosari‐Nasab, Morteza

doi:10.1007/s11240-019-01639-x

Cited by 22 publications

(7 citation statements)

References 29 publications

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“…Earlier studies demonstrated that elicitors are very effective for the enhancement of cell growth and increase metabolites accumulation. Furthermore, the type of elicitors, concentration and exposure period favours the biomass accumulation in cell suspension culture (Salma et al, 2018;Zare-Hassani et al, 2019;Açıkgöz, 2020).…”

Section: Resultsmentioning

confidence: 99%

Sodium nitroprusside enhances biomass and gymnemic acids production in cell suspension of Gymnema sylvestre (Retz.) R.Br. ex. Sm.

Mahendran

Kumar

Verma

et al. 2021

Plant Cell Tiss Organ Cult

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Gymnema sylvestre (Retz.) R.Br. ex Sm. is widely used as an e cient Ayurvedic traditional medicine for the treatment of diabetes. Phytochemical investigations of this plant showed gymnemic acids (a group of triterpenoid saponins) as the main active components. The present study aims to investigate the effectiveness of sodium nitroprusside (SNP) treatment for enhancement of cell suspension culture biomass and to evaluate their deacylgymnemic acid, gymnemagenin, gymnemic acid IV and gymnemic acid XVII contents of G. sylvestre. Callus was obtained from in vitro derived leaves of G. sylvestre on MS medium forti ed with 3.0 mg/L 2, 4-D (2, 4-dichlorophenoxyacetic acid) and 2.0 mg/L Kn (Kinetin), and the same were used further to produce cell suspension cultures. Cell suspensions were exposed to different concentrations of SNP (5, 10, 20 and 40 µM) and data were collected at 20, 30 and 40 days. Out of the tested concentrations, 20 µM SNP had the highest level of cell culture growth (398.94 ± 8.32 g/L FCW and 40.00 ± 0.75 g/L DCW) on 40-day as compared to control. High-performance liquid chromatography analysis showed that maximum accumulation of deacylgymnemic acid (5.51 mg/g DCW), gymnemagenin (2.80 mg/g DCW) and gymnemic acid XVII (2.08 mg/g DCW) in 20 µM SNP treatment which is (13.43, 13.86 and17.33 folds) higher than the respective control at 40 days exposure. This research suggests that G. sylvestre cell suspension culture with optimal SNP elicitation treatment could be used as a good strategy for the large-scale production of these secondary metabolites at the industrial level.

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Section: Resultsmentioning

confidence: 99%

Sodium nitroprusside enhances biomass and gymnemic acids production in cell suspension of Gymnema sylvestre (Retz.) R.Br. ex. Sm.

Mahendran

Kumar

Verma

et al. 2021

Plant Cell Tiss Organ Cult

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“…Foliar monoterpene and sesquiterpene emissions from many plant species increase after MeJA application (Wuyun et al, 2023; Cappellari et al, 2019). MeJA‐mediated elicitation is utilized in many commercial strategies for the overproduction of naturally active plant products (Jeyasri et al, 2023). Wounding induces the synthesis of jasmonate, which mediates the defence response in plants (Turner et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Methyl jasmonate inducible UGT79A18 is a novel glycosyltransferase involved in the bacoside biosynthetic pathway in Bacopa monnieri

Kumar,

Singh,

Lahane

et al. 2024

Physiologia Plantarum

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Bacosides are dammarane‐type triterpenoidal saponins in Bacopa monnieri and have various pharmacological applications. All the bacosides are diversified from two isomers, i.e., jujubogenin and pseudojujubogenin. The biosynthetic pathway of bacoside is not well elucidated. In the present study, we characterized a UDP‐glycosyltransferase, UGT79A18, involved in the glycosylation of pseudojujubogenin. UGT79A18 shows higher expression in response to 5 h of wounding, and 3 h of MeJA treatment. The recombinant UGT79A18 shows in vitro activity against a wide range of flavonoids and triterpenes and has a substrate preference for protopanaxadiol, a dammarane‐type triterpene. Secondary metabolite analysis of overexpression and knockdown lines of UGT79A18 in B. monnieri identify bacopasaponin D, bacopaside II, bacopaside N2 and pseudojujubogenin glucosyl rhamnoside as the major bacosides that were differentially accumulated. In the overexpression lines of UGT79A18, we found 1.7‐fold enhanced bacopaside II, 8‐fold enhanced bacopasaponin D, 3‐fold enhanced pseudojujubogenin glucosyl rhamnoside, and 1.6‐fold enhanced bacopaside N2 content in comparison with vector control plant, whereas in the knockdown lines of UGT79A18, we found 1.4‐fold reduction in bacopaside II content, 3‐fold reduction in the bacopasaponin D content, 2‐fold reduction in the pseudojujubogenin glucosyl rhamnoside content, and 1.5‐fold reduction in bacopaside N2 content in comparison with vector control. These results suggest that UGT79A18 is a significant UDP glycosyltransferase involved in glycosylating pseudojujubogenin and enhancing the pseudojujubogenin‐derived bacosides.

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“…SMRGs including MYB15 ( Marchev et al, 2020 ), NAC29 ( Huang et al, 2015 ; Wang T. T. et al, 2019 ), TLP3 ( Ibrahim and Jaafar, 2013 ; Bao et al, 2014 ), HFB2A ( Paupiere et al, 2020 ), WRKY27 , OLE16 ( Shao et al, 2019 ), BBE8 ( Daniel et al, 2017 ), LEA3 ( Zhao et al, 2011 ), CHSY ( Dao et al, 2011 ), DRE1D ( Agarwal et al, 2017 ), Malate synthase ( de Kraker and Gershenzon, 2011 ), cycloartenol synthase ( Jin et al, 2017 ) and CASL1 ( Taura et al, 2007 ) in drought enhances secondary metabolite responsive pathways and exert to provide tolerance in the adverse condition in drought stress condition. In salt, several identified genes such as TLP ( Zare-Hassani et al, 2019 ; de Jesus-Pires et al, 2020 ), WRKY6 ( Robatzek and Somssich, 2001 ; Afrin et al, 2015 ), MMP-2 ( Guo et al, 2019 ), peroxidase 53 ( Novo-Uzal et al, 2014 ), bHLH25 ( Goossens et al, 2019 ; Meraj et al, 2020 ; Yang et al, 2020 ), malate synthase ( Brito et al, 2020 ), carboxylesterase ( Wang et al, 2015 ; Cao et al, 2019 ), 1-deoxy-D-xylulose-5-phosphate synthase 2 ( Paetzold et al, 2010 ), NAC ( He et al, 2019 ), DCS2 ( George et al, 2017 ), XTH ( Choi et al, 2011 ; Guerriero et al, 2018 ), AtHB-7 ( Olsson et al, 2004 ), NCED5 ( Udomchalothorn et al, 2017 ), CAS ( Shirazi et al, 2019 ), ERF ( Meraj et al, 2020 ), galactinol synthase ( Patel et al, 2020 ), EXPA7 ( Jadamba et al, 2020 ) and glutathione S-transferase ( Nazar et al, 2011 ; Czerniawski and Bednarek, 2018 ) are SMRGs exhibited crucial role in providing resistance under salt stress condition. Overall, these putative hub genes might have putative functions in drought and salt stress responses in cotton.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Meta-Analysis Associated Targeting Hub Genes and Pathways of Drought and Salt Stress Responses in Cotton (Gossypium hirsutum): A Network Biology Approach

et al. 2022

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Abiotic stress tolerance is an intricate feature controlled through several genes and networks in the plant system. In abiotic stress, salt, and drought are well known to limit cotton productivity. Transcriptomics meta-analysis has arisen as a robust method to unravel the stress-responsive molecular network in crops. In order to understand drought and salt stress tolerance mechanisms, a meta-analysis of transcriptome studies is crucial. To confront these issues, here, we have given details of genes and networks associated with significant differential expression in response to salt and drought stress. The key regulatory hub genes of drought and salt stress conditions have notable associations with functional drought and salt stress-responsive (DSSR) genes. In the network study, nodulation signaling pathways 2 (NSP2), Dehydration-responsive element1 D (DRE1D), ethylene response factor (ERF61), cycling DOF factor 1 (CDF1), and tubby like protein 3 (TLP3) genes in drought and tubby like protein 1 (TLP1), thaumatin-like proteins (TLP), ethylene-responsive transcription factor ERF109 (EF109), ETS-Related transcription Factor (ELF4), and Arabidopsis thaliana homeodomain leucine-zipper gene (ATHB7) genes in salt showed the significant putative functions and pathways related to providing tolerance against drought and salt stress conditions along with the significant expression values. These outcomes provide potential candidate genes for further in-depth functional studies in cotton, which could be useful for the selection of an improved genotype of Gossypium hirsutum against drought and salt stress conditions.

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The effects of methyl jasmonate and salicylic acid on the production of secondary metabolites in organ culture of Ziziphora persica

Cited by 22 publications

References 29 publications

Sodium nitroprusside enhances biomass and gymnemic acids production in cell suspension of Gymnema sylvestre (Retz.) R.Br. ex. Sm.

Sodium nitroprusside enhances biomass and gymnemic acids production in cell suspension of Gymnema sylvestre (Retz.) R.Br. ex. Sm.

Methyl jasmonate inducible UGT79A18 is a novel glycosyltransferase involved in the bacoside biosynthetic pathway in Bacopa monnieri

Transcriptome Meta-Analysis Associated Targeting Hub Genes and Pathways of Drought and Salt Stress Responses in Cotton (Gossypium hirsutum): A Network Biology Approach

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