The effects of microtubule and microfilament disrupting agents on cytoskeletal arrays and wall deposition in developing cotton fibers

Seagull, Robert W.

doi:10.1007/bf01326634

Cited by 143 publications

(90 citation statements)

References 60 publications

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“…Work with both developing cotton fibers and tracheary elements suggests that the actin cytoskeleton may also play a role in this process. Treating such cells with cytochalasins, which causes depolymerization of actin filaments, also disrupts MT and microfibril patterns (Kobayashi et al, 1987(Kobayashi et al, , 1988Seagull, 1990). These results suggest that actin may play a primary role in setting the pattern of the cortical MT network, which in turn sets the pattern of cellulose deposition.…”

Section: Role Of the Cytoskeleton In Celwlose Synthesismentioning

confidence: 90%

Cellulose biosynthesis.

1995

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Section: Role Of the Cytoskeleton In Celwlose Synthesismentioning

confidence: 90%

Cellulose biosynthesis.

1995

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“…Disruption of actin filaments with cytochalasin D interfered with reorganization and/or stability of microtubules in onion mitotic cells (Eleftheriou and Palevitz, 1992) and during the development of cotton fibers (Seagull, 1990) and wheat mesophyll cells (Wernicke and Jung, 1992). However, we do not consider the role of actin filaments in guard cells in such a connection because the changing state of actin filaments did not affect the well-organized microtubules in guard cells.…”

Section: Discussionmentioning

confidence: 99%

Actin Filaments of Guard Cells Are Reorganized in Response to Light and Abscisic Acid

1997

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A set of guard cells surrounding stomata of terrestrial plants function much like sliding doors in a building, opening to allow the CO, uptake required for photosynthesis and closing to reduce water loss during periods of water deficit. Such regulation is initiated by sensing environmental and interna1 stimuli such as light, humidity, CO,, and the plant-stress hormone ABA, and is accomplished by osmotic volume changes of the cells. Previous studies have implicated heterotrimeric G-proteins, the H+ pump, and the movement of various ions regulated by ion channels in these processes (for review, see Assmann, 1993). Thus, guard cells provide an ideal system in which to examine whether other molecules, including cytoskeletal elements, take part in plant signaling and, if so, how they interact with better-characterized ones.Actin filaments and microtubules are dynamic cellular components; they disassemble into their building units,

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“…For example, cytochalasin, a microfilament-disrupting drug, prevents the microtubule preprophase band from narrowing in Allium cepa (Eleftheriou and Palevitz, 1992). In addition, long-term treatment with a low level of cytochalasin causes transverse microtubule arrays to reorient into an oblique alignment in growing cotton (Gossypium hirsutum) hairs (Seagull, 1990). These results suggest that microfilaments can influence microtubule organization.…”

Section: Introductionmentioning

confidence: 90%

The Type IIArabidopsisFormin14 Interacts with Microtubules and Microfilaments to Regulate Cell Division

et al. 2010

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Formins have long been known to regulate microfilaments but have also recently been shown to associate with microtubules. In this study, Arabidopsis thaliana FORMIN14 (AFH14), a type II formin, was found to regulate both microtubule and microfilament arrays. AFH14 expressed in BY-2 cells was shown to decorate preprophase bands, spindles, and phragmoplasts and to induce coalignment of microtubules with microfilaments. These effects perturbed the process of cell division. Localization of AFH14 to microtubule-based structures was confirmed in Arabidopsis suspension cells. Knockdown of AFH14 in mitotic cells altered interactions between microtubules and microfilaments, resulting in the formation of an abnormal mitotic apparatus. In Arabidopsis afh14 T-DNA insertion mutants, microtubule arrays displayed abnormalities during the meiosis-associated process of microspore formation, which corresponded to altered phenotypes during tetrad formation. In vitro biochemical experiments showed that AFH14 bound directly to either microtubules or microfilaments and that the FH2 domain was essential for cytoskeleton binding and bundling. However, in the presence of both microtubules and microfilaments, AFH14 promoted interactions between microtubules and microfilaments. These results demonstrate that AFH14 is a unique plant formin that functions as a linking protein between microtubules and microfilaments and thus plays important roles in the process of plant cell division.

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The effects of microtubule and microfilament disrupting agents on cytoskeletal arrays and wall deposition in developing cotton fibers

Cited by 143 publications

References 60 publications

Cellulose biosynthesis.

Cellulose biosynthesis.

Actin Filaments of Guard Cells Are Reorganized in Response to Light and Abscisic Acid

The Type IIArabidopsisFormin14 Interacts with Microtubules and Microfilaments to Regulate Cell Division

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