The Effects of Microwave-Irradiated Fixation for Postmortem Changes of the Kidney

Yabuki, Akira; Matsumoto, Mitsuharu; Taniguchi, Kazuyuki; Suzuki, Syusaku

doi:10.1292/jvms.66.705

Cited by 1 publication

(1 citation statement)

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“…9 Alternatively, processing with microwaves has exhibited progress recently, but the technique can cause uneven fixation and tissue damage. 10,11 Another recent method reported to have superior tissue fixation qualities first incubates tissue in cold NBF, followed by a short incubation in warm NBF. The cold step suppresses enzymatic actions associated with analyte degradation while simultaneously facilitating diffusion of formaldehyde throughout the tissue, and the ensuing warm step rapidly forms formaldehyde linkages to complete the fixation process.…”

Section: Introductionmentioning

confidence: 99%

Active monitoring of formaldehyde diffusion into histological tissues with digital acoustic interferometry

et al. 2016

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Abstract. The preservation of certain labile cancer biomarkers with formaldehyde-based fixatives can be considerably affected by preanalytical factors such as quality of fixation. Currently, there are no technologies capable of quantifying a fixative's concentration or the formation of cross-links in tissue specimens. This work examined the ability to detect formalin diffusion into a histological specimen in real time. As formaldehyde passively diffused into tissue, an ultrasound time-of-flight (TOF) shift of several nanoseconds was generated due to the distinct sound velocities of formalin and exchangeable fluid within the tissue. This signal was resolved with a developed digital acoustic interferometry algorithm, which compared the phase differential between signals and computed the absolute TOF with subnanosecond precision. The TOF was measured repeatedly across the tissue sample for several hours until diffusive equilibrium was realized. The change in TOF from 6-mm thick ex vivo human tonsil fit a single-exponential decay (R 2 adj ≥ 0.98) with rate constants that varied drastically spatially between 2 and 10 h (σ ¼ 2.9 h) due to substantial heterogeneity. This technology may prove essential to personalized cancer diagnostics by documenting and tracking biospecimen preanalytical fixation, guaranteeing their suitability for diagnostic assays, and speeding the workflow in clinical histopathology laboratories. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Introductionmentioning

confidence: 99%