The Effects of Mobile Phone Radiofrequency Electromagnetic Fields on β-Amyloid-Induced Oxidative Stress in Human and Rat Primary Astrocytes

Tsoy, Andrey; Saliev, Timur; Abzhanova, Elvira; Turgambayeva, Anel; Kaiyrlykyzy, Aiym; Akishev, Mars; Saparbayev, Samat; Umbayev, Bauyrzhan; Askarova, Sholpan

doi:10.1016/j.neuroscience.2019.03.058

Cited by 26 publications

(26 citation statements)

References 51 publications

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“…Radiofrequency electromagnetic radiation belongs to the non-ionizing part of the electromagnetic spectrum, as all UV light greater than 100 nm wavelength are too weak to ionize molecules. Permissible RF levels proposed by ICNIRP (1998, 2020) are based on thermal effects only, whereas, a large number of publications suggest non-thermal effects, mainly through free radicals and oxidative stress [61][62][63][64][65][66]. Our work is based on the assumption of cooperative (additive), synergistic, or possible protective mechanisms of successive exposure to RF and UV, since UV radiation is able to induce adverse biological effects, per se.…”

Section: Discussionmentioning

confidence: 98%

Evaluation of Inflammation by Cytokine Production Following Combined Exposure to Ultraviolet and Radiofrequency Radiation of Mobile Phones on 3D Reconstructed Human Skin In Vitro

Szilágyi

Németh

Bakos

et al. 2020

IJERPH

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The absorption of exposure to radiofrequency (RF) emitted by wireless devices leads to a high specific absorption rate in the skin. Ultraviolet (UV) radiation can induce several damages to the skin. The aim of this study was to examine whether combined, consecutive exposure to solar UV radiation and 1950 MHz RF exposure of third generation (3G) mobile system have any effect on inflammation processes in the skin. Under in vitro experiments, the inflammation process was examined by cytokines (IL-1α, IL-6, and IL-8) and MMP-1 enzyme secretion on 3D full thickness human skin model. The RF exposure was applied before or after UV irradiation, in order to study either the possible cooperative or protective effects of exposure to RF and UV. We did not find changes in cytokines due to exposure to RF alone. The RF exposure did not enhance the effects of UV radiation. There was a statistically not-significant decrease in cytokines when the skin tissues were pre-exposed to RF before being exposed to 4 standard erythemal dose (SED) UV compared to UV exposure alone. We found that RF exposure reduced the previously UV-treated MMP-1 enzyme concentration. This study might support the evaluation of the effects on the skin exposed to microwave radiation of 5G mobile technology.

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Section: Discussionmentioning

confidence: 98%

Evaluation of Inflammation by Cytokine Production Following Combined Exposure to Ultraviolet and Radiofrequency Radiation of Mobile Phones on 3D Reconstructed Human Skin In Vitro

Szilágyi

Németh

Bakos

et al. 2020

IJERPH

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“…The potential mechanisms that RF-EMF acts on the brain indicated by previous studies including influencing the normal functions of the blood-brain barrier (Sirav and Seyhan, 2016;Poulletier de Gannes et al, 2017), inducing neuroinflammation (Lameth et al, 2017), altering the activity of specific calcium channels (Buckner et al, 2015), inducing autophagy (Kim et al, 2018b), and stimulating oxidative stress (Tsoy et al, 2019). Previous studies have also indicated that RF-EMF exposure changes the expression of specific genes related to neuronal development (Nikolova et al, 2005;Zhao et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

1800 MHz Radiofrequency Electromagnetic Field Impairs Neurite Outgrowth Through Inhibiting EPHA5 Signaling

Chen

Deng

et al. 2021

Front. Cell Dev. Biol.

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The increasing intensity of environmental radiofrequency electromagnetic fields (RF-EMF) has increased public concern about its health effects. Of particular concern are the influences of RF-EMF exposure on the development of the brain. The mechanisms of how RF-EMF acts on the developing brain are not fully understood. Here, based on high-throughput RNA sequencing techniques, we revealed that transcripts related to neurite development were significantly influenced by 1800 MHz RF-EMF exposure during neuronal differentiation. Exposure to RF-EMF remarkably decreased the total length of neurite and the number of branch points in neural stem cells-derived neurons and retinoic acid-induced Neuro-2A cells. The expression of Eph receptors 5 (EPHA5), which is required for neurite outgrowth, was inhibited remarkably after RF-EMF exposure. Enhancing EPHA5 signaling rescued the inhibitory effects of RF-EMF on neurite outgrowth. Besides, we identified that cAMP-response element-binding protein (CREB) and RhoA were critical downstream factors of EPHA5 signaling in mediating the inhibitory effects of RF-EMF on neurite outgrowth. Together, our finding revealed that RF-EMF exposure impaired neurite outgrowth through EPHA5 signaling. This finding explored the effects and key mechanisms of how RF-EMF exposure impaired neurite outgrowth and also provided a new clue to understanding the influences of RF-EMF on brain development.

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“…Neural stem cells (NSCs) in adult human brain are able to self-renew and possess multidifferential ability to maintain homeostasis and repair damage after acute central nervous system [16]. Tsoy and col. showed that EMF is able to reduce Aβ42and H 2 O 2 -induced cellular ROS, abrogate Aβ₄₂-induced production of mitochondrial ROS and the co-localization between the cytosolic (p47-phox) and membrane (gp91-phox) subunits of NADPH oxidase, while increasing MMP, and inhibiting H 2 O 2 -induced phosphorylation of p38MAPK and ERK1/2 in primary astrocytes, indicating the therapeutic potential of RF-EMF for the treatment of Alzheimer's disease [17]. Several studies also confirm that low-frequency EMF is a possible non-invasive therapeutic intervention for neurological rehabilitation secondary injury-induced events [18,19].…”

Section: Fig 2 Standard Nano2 Curvementioning

confidence: 89%

Neuroprotective Effects of Low Intensity and Low Frequency Electromagnetic �in vitro� Stimulation on Glial Cells

Dascălu¹,

Serban²,

Vladila³

et al. 2020

Rev. Chim.

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Degenerative neurological diseases (senile dementia, Alzheimer's disease, glaucoma), post-stroke sequelae are increasing as prevalence nowadays, in context of aging in general population, and there are strong evidences that astrocytes may play a significant role in neuroprotection. The study evaluates the effects of low intensity low frequency electromagnetic field (EMF) "in vitro" stimulation (195 mA,(7)(8) on the glial cells, in different conditions, in order to identify the neuroprotective potential. Three cell lines were used for the study: the Clonetics (Lonza) line of normal human astrocytes, U87 glioblastoma tumor cells (ATCC) and L929 murine fibroblasts. The cell cultures were exposed to EMF stimulation for 2 hours/day, for 5 and 10 day, in absence and in the presence of oxidative stress stimuli. Cell viability, apoptotic potential, changes in cell phenotype, stimulation of cell proliferation for normal and malignant transformed cell lines, resilience to oxidative stress were investigated. The viability of the cells was investigated by MTS assay, and the Griess test was used to detect nitrite production as a marker of oxidation and inflammation. Inverted microscopy examination revealed that following exposure to the tested EMF, no morphological changes of the cells were recorded. In none of the cell lines tested, no cytotoxic or cytopathic effects were observed in the cultures exposed under low density conditions. In case of exposure of cell cultures for 10 days to the action of EMF, a viability of 98.2% of glial cells and 97% of exposed fibroblasts is observed, compared to unexposed cells. The viability of the cells exposed to both EMF and oxidative and inflammatory stimuli is increased by 0.96% in the case of H2O2 stimulation and in 3.76% in the case of LPS stimulation, suggesting a possible neuroprotective effect of EMF exposure. Low intensity low frequency EMF "in vitro" stimulation increased viability of the glial cells exposed to oxidative and inflammatory stimuli. No cytotoxic or cytopathic effects were observed in the exposed cultures under conditions of low density. Further tests are needed in order to elucidate the mechanisms of action of EMF on astrocytes, especially on astrocyte-neuron co-cultures to highlight the interactions between these cell types. Confirmation of the neuroprotective effect of low intensity and low frequency EMF opens the way for the development of an innovative, non-invasive therapy.

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The Effects of Mobile Phone Radiofrequency Electromagnetic Fields on β-Amyloid-Induced Oxidative Stress in Human and Rat Primary Astrocytes

Cited by 26 publications

References 51 publications

Evaluation of Inflammation by Cytokine Production Following Combined Exposure to Ultraviolet and Radiofrequency Radiation of Mobile Phones on 3D Reconstructed Human Skin In Vitro

Evaluation of Inflammation by Cytokine Production Following Combined Exposure to Ultraviolet and Radiofrequency Radiation of Mobile Phones on 3D Reconstructed Human Skin In Vitro

1800 MHz Radiofrequency Electromagnetic Field Impairs Neurite Outgrowth Through Inhibiting EPHA5 Signaling

Neuroprotective Effects of Low Intensity and Low Frequency Electromagnetic �in vitro� Stimulation on Glial Cells

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