Objectives: This study aimed to investigate the choroidal thickness in patients with nasal septum deviation using enhanced depth imaging spectral-domain optical coherence tomography (EDI-OCT). Patients and Methods: The prospective case-control study included 106 eyes of 53 patients (29 males, 24 females; mean age: 31.2±10.6 years; range, 18 to 50 years) who were detected to have nasal septum deviation by anterior rhinoscopy and nasal endoscopy between April 2020 and July 2020. Seventy-two eyes of 36 healthy participants (20 males, 16 females; mean age: 28.3±10.1 years; range, 18 to 50 years) were included in the control group. The degree of deviation of patients with septum deviation was graded with the nasal obstruction symptom evaluation (NOSE) scale. Choroidal thickness measurements were performed at subfoveal, temporal, and nasal regions using the EDI-OCT and compared in patients with and without deviation. In addition, the relationship between the severity of septal deviation and choroidal thickness measurements was examined. Results: The mean subfoveal, nasal, and temporal choroidal thicknesses in the nasal septum deviation group were 361.0±99.4 μm, 334.5±101.1 μm, and 349.7±93.6 μm, respectively. The mean subfoveal, nasal, and temporal choroidal thicknesses in the control group were 381.2±75.5 μm, 345.5±72.8 μm, and 368.6±79.2 μm, respectively. There was no significant difference in choroidal thickness measurements between the groups (p>0.05). However, it was observed that choroidal thickness decreased as the NOSE scale grade increased (p<0.05). Conclusion: This study shows that choroidal thickness measurements are similar between patients with nasal septal deviation and healthy participants and demonstrates a negative correlation between the severity of septum deviation and choroidal thickness.