The effects of nitric oxide on the contractility of isolated uterine and aortic rings from pregnant rats

Okawa, Toshiaki; Syal, Ashu S.; Vedernikov, Yuri P.; Saade, George R.; Chwalisż, Kristof; Garfield, Robert E.

doi:10.1016/s0002-9378(98)70071-7

Cited by 17 publications

(24 citation statements)

References 18 publications

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“…Increased expression of oxytocin receptor mRNA, but not prostaglandin F 2a receptor mRNA, during labor occurred in pregnant, but not in non-pregnant, uterine horn (20,21) and the requirement of both hormonal and mechanical factors was postulated (20). Inhibitory effect on uterine contractile activity of NO is accentuated during pregnancy and decreased toward term (2,4,6,16). DEA / NO spontaneously releasing NO with an estimated half-life of about 2.1 min in solution with pH 7.4 at 37°C (22) relaxed vascular smooth muscle (8) and inhibited spontaneous contractions of myometrium during pregnancy, but not at term (2,4,23).…”

Section: Discussionmentioning

confidence: 94%

“…Inhibitory effect on uterine contractile activity of NO is accentuated during pregnancy and decreased toward term (2,4,6,16). DEA / NO spontaneously releasing NO with an estimated half-life of about 2.1 min in solution with pH 7.4 at 37°C (22) relaxed vascular smooth muscle (8) and inhibited spontaneous contractions of myometrium during pregnancy, but not at term (2,4,23). The greater inhibitory effect of DEA / NO on spontaneous uterine contractions in pregnant uterine horn is associated with greater expression of iNOS mRNA in this experiments.…”

Section: Discussionmentioning

confidence: 99%

“…However, it is suggested that this relaxation was independent of the inhibition of phosphodiesterase V (28). On the other hand, the less sensitivity of uterine versus vascular smooth muscle to NO and differential selectivity of NO for inducing relaxation between vascular and non-vascular smooth muscle suggests the differential pharmacological properties of NO (2,29). Therefore, in the uterine tissue, it has yet not been determined if this relaxation by UK-114542 at high concentrations is dependent or independent of the inhibition of selective phosphodiesterase V. The membrane-permeable analog of cGMP, 8-br-cGMP, inhibited contractile activity of rings from both pregnant and non-pregnant uterine horns with no differences in either pD 2 's or maximal response, which is consistent with published data (21).…”

Section: Discussionmentioning

confidence: 99%

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Expression of iNOS mRNA and Inhibitory Effect of NO on Uterine Contractile Activity in Rats Are Determined by Local Rather Than Systemic Factors of Pregnancy

et al. 2004

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Abstract. Our purpose was to investigate whether the local or systemic factors of pregnancy are associated with inducible nitric oxide synthase (iNOS) mRNA expression and to determine the inhibitory effects of pharmacological agents that increase cGMP levels in rat myometrium. iNOS mRNA expression was determined in uterine tissues from nonpregnant rats and on day 17 of gestation in the pregnant and non-pregnant uterine horns by RT-PCR. In addition, uterine rings from the pregnant and non-pregnant uterine horns were placed in Krebs-Henseleit solution for isometric recordings of spontaneous contractions. Concentration-inhibition relationships to diethylamine / nitric oxide complex, 8-bromo-cGMP, and the selective phosphodiesterase V inhibitor were obtained. Compared to nonpregnant rats, expression of iNOS mRNA in myometrium increased during pregnancy, which was maximal on day 17, followed by a decrease on day 21 of gestation. Expression of iNOS mRNA at day 17 of gestation was greater in pregnant uterine horns than in nonpregnant ones. Maximal inhibition of phosphodiesterase V and increasing cGMP induced similar inhibition of spontaneous contractions in nonpregnant and pregnant uterine horns, while NO induced less inhibition in the former. The results suggest that the local pregnancy factor is needed for signal transduction from NO to soluble guanylate cyclase at a time when maximal expression of iNOS mRNA is evident.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Expression of iNOS mRNA and Inhibitory Effect of NO on Uterine Contractile Activity in Rats Are Determined by Local Rather Than Systemic Factors of Pregnancy

et al. 2004

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“…The increase in NO via iNOS during pregnancy and from NO donors can inhibit uterine contraction [12,13] . However, in these infl ammatory states, the increase in NO could not prevent the production of uterine contractions.…”

Section: Discussionmentioning

confidence: 99%

“…Increase of NO in the uterus, mainly via activation of inducible NO synthase (iNOS) and NO donors, inhibits uterine contraction, while its downregulation and degradation exert opposite enhancing effects [12,13] .…”

mentioning

confidence: 99%

Reduced Nitric Oxide in Amniotic Fluid of Patients with Chorioamnionitis

Daoud

Suzuki²,

Yamamoto³

et al. 2005

Fetal Diagn Ther

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Objective: Levels of nitric oxide (NO) and cytokines were assessed in amniotic fluid obtained from patients with severe chorioamnionitis (CAM) and appropriate controls. Methods: Amniotic fluid was obtained from 12 patients with CAM (17–24 weeks of gestation) and 89 patients undergoing diagnostic amniocentesis (16–18 weeks of gestation). The concentrations of NO, interleukin-6 (IL-6), and leukocyte elastase (LE) in amniotic fluid were then measured and compared. Results: The concentrations of NO, IL-6, and LE were all higher in CAM cases than in normal pregnant women. Furthermore, an inverse correlation between NO and LE was suggested in the CAM group. Conclusions: These results indicate that in severe CAM, the action of NO might be reduced, not only due to blockage of action but also by degradation, despite increased production.

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Use of sublingual isosorbide dinitrate tablet for manual extraction of a retained placenta

Okawa

Takano

Takahashi

et al. 2002

Arch Gynecol Obstet

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The effects of nitric oxide on the contractility of isolated uterine and aortic rings from pregnant rats

Cited by 17 publications

References 18 publications

Expression of iNOS mRNA and Inhibitory Effect of NO on Uterine Contractile Activity in Rats Are Determined by Local Rather Than Systemic Factors of Pregnancy

Expression of iNOS mRNA and Inhibitory Effect of NO on Uterine Contractile Activity in Rats Are Determined by Local Rather Than Systemic Factors of Pregnancy

Reduced Nitric Oxide in Amniotic Fluid of Patients with Chorioamnionitis

Use of sublingual isosorbide dinitrate tablet for manual extraction of a retained placenta

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