The effects of nitrogen and potassium nutrition on the growth of nonembryogenic and embryogenic tissue of sweet orange (Citrus sinensis (L.) Osbeck)

Niedz, Randall P.; Evens, Terence J.

doi:10.1186/1471-2229-8-126

Cited by 31 publications

(13 citation statements)

References 25 publications

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“…Cell elongation was found to be associated with increased potassium [42]. Recently, Niedz and Evens [43] observed that K + nutrition is an important regulator for growth of embryogenic callus of sweet orange (Citrus sinesis (L.) Osbeck). In the present investigation, changes in the concentration of K in medium (potassium iodide form) led to a significant enhancement in the embryo yield and regeneration efficiency of P. ovata.…”

Section: Discussionmentioning

confidence: 98%

Formulation of Nutrient Medium for In Vitro Somatic Embryo Induction in Plantago ovata Forsk

Saha

Bandyopadhyay

Raychaudhuri

2010

Biol Trace Elem Res

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A nutrient medium has been formulated by altering the macro- and micro-elemental concentration in the culture medium for in vitro somatic embryo induction of economically important medicinal plant Plantago ovata Forsk .A comparison was made between induced embryos with normal embryos (produced in Murashige and Skoog (MS) medium) to observe frequency of embryo induction and also to determine regeneration efficiency. In the present investigation, three different media have been formulated. Among them, FM3 (formulated media, treatment 3) was the most suitable for increasing the frequency of somatic embryo production and regeneration of P. ovata Forsk. Better result was obtained using formulated medium than with MS medium.

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Section: Discussionmentioning

confidence: 98%

Formulation of Nutrient Medium for In Vitro Somatic Embryo Induction in Plantago ovata Forsk

Saha

Bandyopadhyay

Raychaudhuri

2010

Biol Trace Elem Res

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“…R 2 , adjusted-R 2 (R adj 2 ), and predicted-R 2 (R pred 2 ), were estimated for each selected model (Myers and Montgomery 2002). Detailed descriptions of the statistical (Niedz and Evens 2008;. The software application Design-Expert® 7 (Stat-Ease, Inc, Minneapolis, MN) was used for experimental design construction, model evaluation, and analysis.…”

Section: Discussionmentioning

confidence: 99%

In vitro shoot growth of Brugmansia × candida Pers

Niedz

Evens

Hyndman

et al. 2011

Physiol Mol Biol Plants

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The objective of this study was to improve the growth of in vitro shoot cultures of Brugmansia × candida 'Creamsickle'. Several mineral nutrient experiments were conducted to determine the effect of NH4 (+), NO3 (-), K(+), FeSO4/EDTA, ZnSO4, MnSO4, and CuSO4 on quality, leaf width and length, size and weight of shoot mass, and shoot number. The experiment to determine the levels of NH4 (+), NO3 (-), and K(+), was conducted as a 2-component NH4 (+): K(+) mixture crossed by [NO3 (-)] and resulted in an experimental design free of ion confounding and capable of separating the effects of proportion and concentration. The results of the NH4 (+)-K(+)-NO3 (-) experiment revealed a region in the design space where growth was significantly improved; the region generally had lower total nitrogen and lower NH4 (+):K(+) ratios than MS medium. The experiments to determine the appropriate levels of Fe, Zn, Mn, and Cu were conducted at six log levels ranging from 0 to 1 mM. Of the four metal salts tested, MnSO4 had the least effect on in vitro shoot growth and its concentration was reduced from 0.1 mM (MS level) to 0.001 mM. CuSO4 had large effects on in vitro shoot growth and was increased from 0.0001 mM to 0.001 mM. A 2-level factorial of NH4 (+)-K(+)-NO3 (-), FeSO4/EDTA, and ZnSO4 was conducted and several formulations identified for their improvements of quality and growth. In addition to the changes to MnSO4 and CuSO4, these formulations were characterized by lower levels of NH4 (+), K(+), NO3 (-) and Zn, and higher levels of FeSO4/EDTA. Overall, several nutrient formulations were identified as superior to MS medium for growth of in vitro shoot cultures of B. 'Creamsickle'.

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“…Nitrogen form and concentration Historically, in cereal tissue culture, MS medium has been commonly used for plant regeneration and it contains a NH 4 + /K + ratio of 1. For this experiment, the two-component mixture of NH 4 + and K + and one quantitative factor NO 3 − amount were tested using a mixture amount design similar to Niedz and Evens (2008). Factor levels are shown in Table 2.…”

Section: Methodsmentioning

confidence: 99%

“…Transplanted T0 plantlets are grown to maturity, pollinated, and seed is harvested after approximately 3 to 4 mo problem of ion confounding between the effect of the Nsource, and the counter ion in that salt. Varying the ratio of NH 4 + /K + and the total nitrate concentration [NO 3 − ] in a twocomponent mixture, provided a direct estimation of mineral nutrient effects without the ion confounding of a salt-based approach (Niedz and Evens 2008). Potassium nutrition was also considered in this design.…”

Section: Introductionmentioning

confidence: 99%

Use of DoE methodology to optimize the regeneration of high-quality, single-copy transgenic Zea mays L. (maize) plants

Chu

Adelberg

Lowe

et al. 2019

In Vitro Cell.Dev.Biol.-Plant

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The maize Agrobacterium tumefaciens-mediated transformation process normally takes about 10 to 15 wk from embryo infection and co-cultivation to sending "T0" plants to the greenhouse (GH). A new method was developed using the maize transcription factors Babyboom (BBM) and Wuschel2 (WUS2), to stimulate direct transgenic embryo formation and plant regeneration, that bypasses the need for prolonged tissue culture and regeneration from callus. In the present study, a design of experiment (DoE) method was used to test 10 factors to optimize the quality of somatic embryo maturation, root formation, and subsequent plantlet survival, without compromising the molecular event quality. The concentration of NO 3 − and the ratio of NH 4 + to K + had significant effects on the morphology of plantlets derived directly from germinated transgenic embryos. During early development, optimal tissue morphology required a NH 4 + /K + ratio of 1:1 with 20 mM [NO 3 − ], of 14.2 μM 6-benzylaminopurine (BAP), the highest concentration tested, and a low light intensity of 50 μmol m −2 s −1. Later development of rooted shoots required additional macronutrients with reduced NH 4 NO 3 (15 mM NH 4 NO 3 and 25 mM KNO 3), reduced BAP (7.4 μM), and 1 μM abscisic acid (ABA) and at a higher light intensity of 140 μmol m −2 s −1. Using the optimized parameters, the frequency of plants sent to the GH was improved by twofold compared with the current process and the number of single-copy T-DNA events was doubled.

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The effects of nitrogen and potassium nutrition on the growth of nonembryogenic and embryogenic tissue of sweet orange (Citrus sinensis (L.) Osbeck)

Cited by 31 publications

References 25 publications

Formulation of Nutrient Medium for In Vitro Somatic Embryo Induction in Plantago ovata Forsk

Formulation of Nutrient Medium for In Vitro Somatic Embryo Induction in Plantago ovata Forsk

In vitro shoot growth of Brugmansia × candida Pers

Use of DoE methodology to optimize the regeneration of high-quality, single-copy transgenic Zea mays L. (maize) plants

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