The Effects of Oligosaccharide and Spores from Aspergillus niger on the Defence Responses of Taxus chinensis Leaves In vitro

Li, M. Y.; Chen, C.; Yu, Long-Jiang

doi:10.1046/j.1439-0434.2003.00765.x

Cited by 5 publications

(4 citation statements)

References 43 publications

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“…Phenylalanine ammonia lyase was extracted and its activity determined by the modified methods of Li et al (2003). Fresh cells (0.5 g) were homogenized with quartz sand, 10 mL extraction buffer (0.1 m sodium borate, pH 8.0, 5 mm mercaptoethanol) and 0.5 g polyvinylpyrrolidone (PVP).…”

Section: Pal Activity Assaymentioning

confidence: 99%

Influence of Drought on Oxidative Stress and Flavonoid Production in Cell Suspension Culture of Glycyrrhiza inflata Batal

Yang

et al. 2007

Zeitschrift Für Naturforschung C

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The effect of water deficit on flavonoid production and physiological parameters characteristic for oxidative stress were studied in a cell suspension culture of Glycyrrhiza inflata Batal to investigate its drought tolerance. The result indicated that appropriate water deficit enhanced biomass accumulation of 27.1 g L Ð1 and flavonoid production of 151.5 mg L Ð1, which was about 2-fold and 1.5-fold of the control, respectively. But it decreased the water content. Drought stress led to hydrogen peroxide accumulation more than in the control. Moreover, under drought conditions, malondialdehyde content, the activities of catalase and peroxidase increased to a greater extent than the control, and each reached a maximum value of 91.3 μmol g Ð1 dry weight, 85.6 U and 1951 U g Ð1 dry weight per min, which was 1.5-, 1.7-and 3.7-fold of the control, respectively. All above showed that appropriate water deficit could activate the antioxidative defense enzymes system to maintain stability in plants subjected to drought stress. On the contrary, the activity of phenylalanine ammonia lyase of the control increased in company with the biosynthesis of flavonoids, which indicated that phenylalanine ammonia lyase might play an important role in the path of the biosynthesis of flavonoids.

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Section: Pal Activity Assaymentioning

confidence: 99%

Influence of Drought on Oxidative Stress and Flavonoid Production in Cell Suspension Culture of Glycyrrhiza inflata Batal

Yang

et al. 2007

Zeitschrift Für Naturforschung C

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“…CO 2 was entrapped in 2 M KOH and radioactivity counted in a scintillation counter (Beckman LS 6500, Milano, Italia). PAL (EC 4.3.1.5) activity was measured as described by Li et al [2003] with minor modifications. Leaf samples (0.5 g) were ground in a pre-frozen mortar with liquid nitrogen 2.5 ml extraction buffer (0.1 M sodium borate, pH 8.0, containing 5 mM mercaptoethanol).…”

Section: Experimental Designmentioning

confidence: 99%

Extremely low frequency weak magnetic fields enhance resistance of NN tobacco plants to tobacco mosaic virus and elicit stress‐related biochemical activities

Trebbi

Borghini

Lazzarato

et al. 2006

Bioelectromagnetics

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Increasing evidence has accumulated concerning the biological effects of extremely low frequency magnetic fields (ELF-MFs) in different plant models. In the present study, effects of ELF-MFs in tobacco plants reacting to tobacco mosaic virus (TMV) with a hypersensitive response (HR) were evaluated. Plants were exposed for 8 or 24 h (either before or after TMV inoculation) to a static MF, at either -17 or 13 microT, combined with a 10 Hz sinusoidal MF with different intensities (25.6 or 28.9 microT). The working variables were the area and number of hypersensitive lesions in leaves. Following ELF-MFs exposure, an increased resistance was detected, particularly after an 8-h treatment, as shown by the decrease in lesion area and number. Moreover, two enzyme activities involved in resistance mechanisms were analyzed: ornithine decarboxylase (ODC) and phenylalanine ammonia-lyase (PAL). Uninoculated leaves previously exposed to ELF-MFs in general showed a significant increase relative to controls in ODC and PAL activities, in particular for 13 microT static MF plus 28.9 microT, 10 Hz sinusoidal MF (24 h) treatment. In conclusion, ELF-MFs seem to influence the HR of tobacco to TMV, as shown by the increased resistance and changes in ODC and PAL activities, indicating the reliability of the present plant model in the study of bioelectromagnetic interactions.

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“…Other molecules classified as elicitors are also capable of initiating a defence response in the host plant. These molecules are mostly oligosaccharides released by the pathogen or plant cells [6]. Studies have shown the action of oligosaccharides in the case of plant/fungus interaction.…”

Section: Introductionmentioning

confidence: 99%

Effect of the Oligosaccharid Fraction of Fusarium oxysporum f. sp. vasinfectum on the Activity of Phenolic Metabolism Enzymes in Cotton Leaves

Bla,

Laurent,

François

et al. 2024

IJPSS

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The synthesis of secondary metabolites, notably phenolic compounds, is part of the plant's defense reactions. The enzymes of phenolic metabolism can constitute a parameter of measurement of the defense of the plants. The aim of this work is to evaluate the activity of phenolic metabolism enzymes and an antioxidant enzyme after treatment of cotton plants with a fungal elicitor. Cotton plants obtained from the cultivar Y764AG3 were treated with the oligosaccharide fraction (FOS) of Fusarium oxysporum f. sp. Vasinfectum (FOV). The extraction of the enzymes is carried out cold by grinding the leaves in extraction buffer in the presence of PVP (0.5 %) and 0.1 M sodium phosphate buffer. The supernatant obtained after centrifugation of the filtrate was purified with Dowex-2 in order to obtain the purified enzymatic fraction. This fraction was used for the determination of enzyme activity by reading the absorbances. The results showed that the activity of ammonia-lyase activity was higher in the treated plants than in the control. This reflects an increase in the biosynthesis of phenolic compounds in the treated plants. On the other hand, oxidoreductase activity was higher in control plants than in treated plants. This indicates a decrease in the degradation of phenolic compounds in the treated plants. This study revealed the existence of an active phenolic metabolism in the FOS-treated plants. Thus defence mechanisms are activated in plants treated with the oligosaccharide fraction of Fusarium oxysporum f. sp. Vasinfectum.

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The Effects of Oligosaccharide and Spores from Aspergillus niger on the Defence Responses of Taxus chinensis Leaves In vitro

Cited by 5 publications

References 43 publications

Influence of Drought on Oxidative Stress and Flavonoid Production in Cell Suspension Culture of Glycyrrhiza inflata Batal

Influence of Drought on Oxidative Stress and Flavonoid Production in Cell Suspension Culture of Glycyrrhiza inflata Batal

Extremely low frequency weak magnetic fields enhance resistance of NN tobacco plants to tobacco mosaic virus and elicit stress‐related biochemical activities

Effect of the Oligosaccharid Fraction of Fusarium oxysporum f. sp. vasinfectum on the Activity of Phenolic Metabolism Enzymes in Cotton Leaves

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