The effects of on osteogenic differentiation and mineralization of human stem cells derived from the gingiva

Abstract

The aim of the present study is to evaluate the effects of the extract of Pongamia pinnata on the morphology, viability, and differentiation potential of human stem cells derived from the gingiva. Methods: Stem cells obtained from gingivae were cultured in an osteogenic medium in the presence of methanol extract of Pongamia pinnata (PPT) at concentrations ranging from 0.001 to 1%. Evaluations of cell morphology and cellular viability were done at Day 1. Alkaline phosphatase activity assays and Alizarin red S staining were performed to evaluate the osteogenic differentiation of stem cells. Results: The morphology of stem cells in the presence of PPT at final concentrations of 0%, 0.001%, 0.01%, 0.1%, and 1% did not produce any noticeable changes when compared with the untreated control group. Application of PPT produced a significant increase in alkaline phosphatase activity when compared to the control group. The results of the Alizarin Red S staining showed a significant increase of absorbance with the 0.001% group. Conclusions: Based on these findings, it was concluded that PPT could produce beneficial effects on mesenchymal stem cells with enhanced osteogenic differentiation.

Adipose‐derived stem cells‐conditioned medium improved osteogenic differentiation of induced pluripotent stem cells when grown on polycaprolactone nanofibers

“…In addition to the nature of the growth factors, these factors can induce sustained changes on stem cells at the genetic and epigenetic levels that cause the patient faced with more problems (Lunyak & Rosenfeld, 2008;Olynik & Rastegar, 2012). Many studies have been tried to introduce alternative factors form herbal or other biological sources to be useable in the orthopedics clinics (Akbarzadeh et al, 2014;Lee, Uddin, Kim, Choi, & Park, 2017;Mofid et al, 2015;K. H. Park et al, 2018;Udalamaththa, Jayasinghe, & Udagama, 2016).…”

“…In addition to the nature of the growth factors, these factors can induce sustained changes on stem cells at the genetic and epigenetic levels that cause the patient faced with more problems (Lunyak & Rosenfeld, ; Olynik & Rastegar, ). Many studies have been tried to introduce alternative factors form herbal or other biological sources to be useable in the orthopedics clinics (Akbarzadeh et al, ; Lee, Uddin, Kim, Choi, & Park, ; Mofid et al, ; K. H. Park et al, ; Udalamaththa, Jayasinghe, & Udagama, ). During the last decade, the use of stem cell CM has been introduced as one of the alternative options for these factors, given that stem cell medium have many factors that secret from the cells that in addition to having a positive effect on the growth and proliferation of stem cells, have cytokines and other immunological factors, which can cause stem cell escape from the immune system of the patient and it also prevents them from rejection (Pawitan, ; Salgado, Reis, Sousa, & Gimble, ).…”

Bambus nativos: estudo fitoquímico e rastreio de moléculas bioativas com efeito sobre cognição e memória

“…ex Munro Tripathi et al 2015;Pande et al 2018 Bambusa pervariabilis McClure Jia Sun et al 2010;Wang et al 2013 Bambusa polymorpha Munro Thomas et al 2016 Bambusa rutila McClure Gao et al 2012 Bambusa textilis McClure Silva et al 2012;Liu et al 2016 Bambusa tulda Roxb. Pande et al 2017;Lee et al 2018 Bambusoideae are divided into two morphologically distinct habits: woody and herbaceous bamboos. While woody bamboos display a wide range of morphological diversity, they do possess multiple shared characteristics, as strongly lignified culms, specialized culm leaves, complex vegetative branching, outer ligules on the foliage leaves, bisexual flowers, and gregarious monocarpy.…”