The effects of oral carvacrol treatment against H<sub>2</sub>O<sub>2</sub>induced injury on isolated pancreas islet cells of rats

Gul, Asli San Dagli; Fadıllıoğlu, Ersin; Karabulut, İsmail; Yeşilyurt, Ahmet; Delibaşı, Tuncay

doi:10.4161/isl.25519

Cited by 25 publications

(13 citation statements)

References 27 publications

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“…As Reg gene expression was observed in the phase of transient β cell proliferation: for example, in pancreatic islets of diabetic BB/Wor//Tky rats during the remission phase of diabetes [32], in islets of nonobese diabetic mice during active diabetogenesis [33] and pancreatic ductal cells, which are thought to be progenitor cells of β cells, during differentiation and proliferation in a mouse model of autoimmune diabetes [34, 35], and inflammation and cell death in and/or around islets was involved in these cases, we used inflammatory cytokines such as IL-1 β , TNF- α , and IFN- γ [36], as well as pancreatic β cell toxic agents, such as palmitate [37, 38], H 2 O 2 [39, 40], SNAP [41, 42], and STZ [43, 44]. Other factors, which were reported to upregulate Reg gene expression in β cells, such as IL-6 [13, 31], glucocorticoid [13, 31], IL-22 [6, 45], IFN- β [35], PDGF [46], and amino acids [46], were tested.…”

Section: Resultsmentioning

confidence: 99%

Synergistic Activations ofREG IαandREG IβPromoters by IL-6 and Glucocorticoids through JAK/STAT Pathway in Human PancreaticβCells

Yamauchi

Itaya‐Hironaka

Sakuramoto‐Tsuchida

et al. 2015

Journal of Diabetes Research

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Reg (Regenerating gene) gene was originally isolated from rat regenerating islets and its encoding protein was revealed as an autocrine/paracrine growth factor for β cells. Rat Reg gene is activated in inflammatory conditions for β cell regeneration. In human, although five functional REG family genes (REG Iα, REG Iβ, REG III, HIP/PAP, and REG IV) were isolated, their expressions in β cells under inflammatory conditions remained unclear. In this study, we found that combined addition of IL-6 and dexamethasone (Dx) induced REG Iα and REG Iβ expression in human 1.1B4 β cells. Promoter assay revealed that a signal transducer and activator of transcription- (STAT-) binding site in each promoter of REG Iα (TGCCGGGAA) and REG Iβ (TGCCAGGAA) was essential for the IL-6+Dx-induced promoter activation. A Janus kinase 2 (JAK2) inhibitor significantly inhibited the IL-6+Dx-induced REG Iα and REG Iβ transcription. Electrophoretic mobility shift assay and chromatin immunoprecipitation revealed that IL-6+Dx stimulation increased STAT3 binding to the REG Iα promoter. Furthermore, small interfering RNA-mediated targeting of STAT3 blocked the IL-6+Dx-induced expression of REG Iα and REG Iβ. These results indicate that the expression of REG Iα and REG Iβ should be upregulated in human β cells under inflammatory conditions through the JAK/STAT pathway.

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Section: Resultsmentioning

confidence: 99%

Synergistic Activations ofREG IαandREG IβPromoters by IL-6 and Glucocorticoids through JAK/STAT Pathway in Human PancreaticβCells

Yamauchi

Itaya‐Hironaka

Sakuramoto‐Tsuchida

et al. 2015

Journal of Diabetes Research

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“…In one study, oral carvacrol was daily prescribed in rats, and its effect on cell damage induced by H 2 O 2 in isolet pancreas cells was studied. Carvacrol ameliorates the cell viability ratio and protects against lipid peroxidation and protein oxidation induced by H 2 O 2 (Dagli Gul et al ., ). Carvacrol with concentrations of 50, 75, 100, 150, and 200 mg/L was added in cultured human blood cells, and TAC and total oxidative stress (TOS) were assessed to determine its antioxidant effect.…”

Section: Antioxidant Propertiesmentioning

confidence: 97%

“…In this study, it was shown that the cell viability and cell injury induced by H 2 O 2 was reduced with carvacrol pretreatment. However, a dose of 20 mg/kg/day of carvacrol was more effective than its higher doses (Dagli Gul et al ., ). The antioxidant effect of carvacrol was also observed in another study (Giannenas et al ., ).…”

Section: Antioxidant Propertiesmentioning

confidence: 97%

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Antiinflammatory, Antioxidant, and Immunological Effects of Carum copticum L. and Some of Its Constituents

Alavinezhad

Boskabady

2014

Phytotherapy Research

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Carum copticum L. has been used traditionally for its various therapeutic effects. The plant contains various components such as thymol and carvacrol. Different therapeutic effects such as antifungal, antioxidant, antibacterial, antiparasitic, and antilipidemic were described for the plant and its constituents. Therefore, antiinflammatory, antioxidant, and immunological effects of C. copticum and its constituents, thymol and carvacrol, were discussed in the present review. Previous studies have shown potent antiinflammatory, antioxidant, and immunological effects for C. copticum and its constituents, thymol and carvacrol. Therefore, the plant and its constituents have therapeutic values in several inflammatory and immunological disorders as well as in the oxidative stress conditions.

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“…Carvacrol is chemically 2‐methyl‐5‐[1‐methylethyl] phenol, a monoterpenoid essential oil obtained from plant Oreganum vulgarae , oil of Thyme , practiced as a traditional and household medicine in various parts of the world. Studies report that it is able to maintain the levels of GSH, thus strengthening the free radical scavenging arsenal during oxidative stress (Dagli Gul et al , ). Carvacrol was recently proved to mitigate pancreatic injury induced by multiple low doses of cerulein by circumventing the oxidative stress and improvement of pancreatic exocrine function (amylase and lipase levels).…”

Section: Herbal Remedies Used For Treating Pancreatitismentioning

confidence: 99%

The Role of Plant-derived Products in Pancreatitis: Experimental and Clinical Evidence

et al. 2017

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Pancreatitis is a gastrointestinal disease with a worldwide sharp surge during the past decade. Pancreatitis includes acute and chronic subtypes, which are graded based on the amount of pancreatic inflammation. Phytoconstituents represent a promising class of therapeutic agents with wide acceptability not only based on folk practices but sound presence of pharmacological and molecular evidences. Growing research evidence indicates that different molecular mechanisms are involved in their protective effect. Many phytoremedies have been tried for the treatment of pancreatic injuries and have shown success in preclinical animal models of pancreatitis. The literature was largely collected through PubMed and Google scholar database. A large proportion of phytochemicals targets the inflammatory cascade and modulates the overtly acting redox balance among which nuclear factor kappa-light-chain-enhancer of activated B cells is the key molecule. Inhibition of apoptosis (artemisinin, embelin), inflammasome (withaferin A), neutrophil rolling (fucoidan), Ca release (caffeine), mitogen activated protein kinase (guggulsterone) and many other novel mechanisms apart from antioxidant effect have been postulated behind the protective effect of phytoconstituents. The present review deals to fill the gap of hitherto unavailable comprehensive review on various plant products screened for the treatment of pancreatitis. The possible mechanistic profile of these phytochemicals is summarized. Copyright © 2017 John Wiley & Sons, Ltd.

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The effects of oral carvacrol treatment against H₂O₂induced injury on isolated pancreas islet cells of rats

Cited by 25 publications

References 27 publications

Synergistic Activations ofREG IαandREG IβPromoters by IL-6 and Glucocorticoids through JAK/STAT Pathway in Human PancreaticβCells

Synergistic Activations ofREG IαandREG IβPromoters by IL-6 and Glucocorticoids through JAK/STAT Pathway in Human PancreaticβCells

Antiinflammatory, Antioxidant, and Immunological Effects of Carum copticum L. and Some of Its Constituents

The Role of Plant-derived Products in Pancreatitis: Experimental and Clinical Evidence

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The effects of oral carvacrol treatment against H2O2induced injury on isolated pancreas islet cells of rats

Cited by 25 publications

References 27 publications

Synergistic Activations ofREG IαandREG IβPromoters by IL-6 and Glucocorticoids through JAK/STAT Pathway in Human PancreaticβCells

Synergistic Activations ofREG IαandREG IβPromoters by IL-6 and Glucocorticoids through JAK/STAT Pathway in Human PancreaticβCells

Antiinflammatory, Antioxidant, and Immunological Effects of Carum copticum L. and Some of Its Constituents

The Role of Plant-derived Products in Pancreatitis: Experimental and Clinical Evidence

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Product

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The effects of oral carvacrol treatment against H₂O₂induced injury on isolated pancreas islet cells of rats