The Effects of Ortho Silicone Applications on the Acclimatization Process of Grapevine Rootstocks

Abstract

Micropropagation is a tool for large-scale reproduction of planting material for viticulture sustainability. Successful micropropagation depends on the continued productivity of plantlets during the acclimatization phase. Due to high humidity in the culture container and free water in the environment, it causes rapid water loss and drying of plantlets with watery stems and leaves, poorly developed cuticle, large intercellular space, and incomplete stomata. Acclimatization of in vitro grown plantlets is often difficult. Silicon is gaining in importance as a useful tool in coping with multiple stress factors in different plant species, due to its contribution to the formation of the cuticle of plants, its mechanical resistance to biotic and abiotic stress, and its contribution to the flow of water through stomata and plant surfaces. In this study, the effects of 500 and 1000 µL ortho silicon applications at the acclimatization stage on in vitro propagated plantlets of 41B, 110R and Fercal grapevine rootstocks were evaluated by examining their survival rates. In the in vitro propagation process, rooting rates were listed as Fercal (64%) and 110R (32%) and 41B (28%) according to rootstocks, while root numbers were listed as Fercal (11.8), 41B (8.5), 110R (3.6). Genotypic differences were determined in the effects of silicon on plantlets in the acclimation process. In 110R and Fercal control plantlets, all plantlets were lost during acclimatization. Of the plantlets treated with 1000 µL SiO2, 110R 66% Fercal 88% and in 41B, the control, 500 and 1000 µL SiO2 applied plantlets survived 100%, 66% and 66%, respectively. 1000 µL SiO2 dose was more effective on survival rates than 500 µL applications. In subsequent studies, it was found that 1000 µL SiO2 applications could be used for practical success in grapevine genotypes that had problems in acclimatization and in other in vitro propagation studies.

The effect of sodium nitroprusside on the vegetative development of Aronia melanocarpa [Michx.] Elliot under in vitro conditions

“…The pH of the nutrient medium was adjusted to 5.8. To prevent bacterial contamination in the nutrient medium, 1 ml/l of Plant Preservative Mixture (PPM) was added (Ak et al, 2021;Babu et al, 2022;Kara et al, 2022;Ekinci et al, 2023). The shoots were transferred to a tillering medium containing 3% sucrose, 3 mg/l BAP, 1 mg/l kinetin, 1 ml/l PPM, and 0.7 g/l agar for proliferation.…”

“…The mentioned cytokinins and auxins are essential plant growth regulators used to obtain a large number of plants and for rooting. However, in recent times, various plant growth-promoting molecules have been used (George et al, 2008;Sezgin and Dumanoğlu, 2009;Kara et al, 2022;Şaşkın et al, 2022).…”

Foliar Applications of Calcium, Silicon and Their Combination: A Tool to Improve Grape Composition and Quality

“…On the other hand, Si is a nonessential element that has been found to be beneficial for improving crop yield and quality and also plant tolerance to various abiotic and biotic stress factors [18]. The effect of a Si supply on grapevine has been assessed in a few investigations [19][20][21], which reported positive changes in grape quality and must composition. Silicon is often provided as foliar sprays that have been shown to induce positive plant responses in terms of improved quality, yield and pest and disease tolerance [22,23].…”