The effects of pancreozymin and of vagal nerve stimulation upon the histological appearance of the pancreas

Harper, A. A.; Mackay, I. F. S.

doi:10.1113/jphysiol.1948.sp004252

Cited by 34 publications

(12 citation statements)

References 5 publications

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“…The photocell was affected only when the blood in the vessels was replaced by intra-arterial injections of saline, and did not respond to back injection of saline into the pancreatic duct, nor to obstruction to the outflow of pancreatic juice. Histological observations by Harper & MacKay (1948) have shown that pancreozymin causes a decrease in the numbers of secretory granules in the cells: this would cause a decrease in opacity of the tissue instead of the increase observed in our experiments.…”

Section: The Effect Of Injectionsmentioning

confidence: 42%

Some observations on changes in the blood content of the cat's pancreas during activity

Holton¹,

Jones²,

Löwe³

1960

The Journal of Physiology

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Section: The Effect Of Injectionsmentioning

confidence: 42%

Some observations on changes in the blood content of the cat's pancreas during activity

Holton¹,

Jones²,

Löwe³

1960

The Journal of Physiology

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“…Pancreozymin increases the enzyme output by the pancreas without affecting the volume of the juice (Harper and Mackay, 1948). In our experiment, despite strong stimulation by secretin and pancreozymin, some acinar cells retained zymogen granules adjacent to the empty lumen, as well as around the islets.…”

Section: Discussioncontrasting

confidence: 44%

“…Pancreozymin increases the enzyme output by the pancreas without affecting the volume of the juice (Harper and Mackay, 1948 Almeida and Grossman (1952) found that a diminished volume paralleled reduced bicarbonate, amylase, trypsin, and lipase excretion after ethionine given to dogs when pancreatic function was stimulated by secretin and urecholine. Vcghelyi and Kemeny (1962) showed that when albino rats were fed a methionine-free diet and then given secretin and pancreozymin there was a reduced concentration of ribonuclease and trypsin followed by a loss of lipase and chymotrypsin; amylase production, however, remained within normal limits.…”

Section: Discussionmentioning

confidence: 99%

Alterations of the enzyme output in ethionine pancreatitis

Müller-Wieland¹

1964

Gut

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From Manchester Royal Infirmary EDITORIAL SYNOPSIS In this study of pancreatitis induced by ethionine the outputs of amylase, trypsin, and lipase were diminished simultaneously.The pancreatic enzymes, amylase, trypsinogen, and lipase, are excreted concurrently in healthy man (Lagerlof, 1942;Burton, Evans, Harper, Howat, Oleesky, Scott, and Varley, 1960). In some cases of chronic pancreatitis a loss of activity of one enzyme has been reported while the concentration of the other two has not been affected (Lagerldf, 1939;Diamond and Siegel, 1941). In the present study ethionine was given to produce pancreatitis in rats. Using this model experiments were designed to investigate the pattern of the output of enzymes in the ethionine-poisoned state. METHODSMale rats only were used, because an excessive fatty degeneration of the liver occurs in female rats in the early stage of ethionine toxicity (Farber, Koch-Weser, and Popper, 1951, Becker;1957).Sixty-one male albino rats ranging in weight from 200 to 300 g. were injected subcutaneously with 4 mg. atropine. Fifteen minutes later light ether anaesthesia was induced, the abdomen opened and anaesthesia continued by the injection of 0-75 ml. of a 5 % urethane solution into the caecum. After tying off the pylorus the main bile duct was occluded by a ligature tied close to the hilum of the liver, and cannulated by a polyethylene catheter (0-98 mm. o.d.) introduced close to the duodenal wall. Samples of pancreatic juice from the numerous pancreatic ducts entering the main bile duct were drained by continuous suction using a negative pressure of I to 2 cm. of water.Exocrine pancreatic secretion was stimulated by secretin and pancreozymin (Boots). For the first 60 minutes 2 units of secretin (Crick, Harper, and Raper, 1949) were infused into the jugular vein every five minutes; for the subsequent 30 minutes 2 units of both secretin and pancreozymin were given every five minutes.Pancreatic juice was fractionated into glass tubes surrounded by ice, the volume measured, and the specimens kept in a deep freeze chamber until amylase activity was Pancreatitis was induced by ethionine. Four animals (group 10) were given ethionine two hours before the test by continuous infusion. Ethionine was also given to other batches of rats intraperitoneally. In the first series (groups 1 to 5) the dose was increased from 0-33 to 1-66 mg./g. of body weight. Each injection contained 0-33 mg./g. body weight and in 24 hours not more than 1-00 mg./g. body weight was given. Pancreatic function was estimated 12 hours after the last injection. The second series was designed to study recovery from ethionine pancreatitis. In groups 3, 6, 7, and 8 the animals were each given the same dose of 1-0 mg./g. body weight of ethionine and pancreatic function was studied at intervals of 24, 48, 72, and 120 hours, after the initial injection of ethionine.In order to investigate the early changes in ethionine pancreatitis, in group 10 033 mg./g. body weight of ethionine was administered intravenously and the test...

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“…With acetylcholine the rates of both of these phenomena decreased with time (Hokin & Hokin, 1953), although the cell still had an appreciable enzyme content after incubation. Harper & MacKay (1948) found that the rate of enzyme secretion in response to pancreozymin in vivo was linear over a 2 hr period, but that the increased output of enzymes in response to vagal stimulation declined progressively after the first half-hour. They were unable to attribute any physiological significance to this as they point out that the diminution in enzyme output might have been due either to general deterioration of the animal's condition or to damage to the vagal trunk, although there were no obvious signs of these.…”

Section: Resultsmentioning

confidence: 97%

The actions of pancreozymin in pancreas slices and the role of phospholipids in enzyme secretion

Hokin¹,

Hokin²

1956

The Journal of Physiology

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The effects of pancreozymin and of vagal nerve stimulation upon the histological appearance of the pancreas

Cited by 34 publications

References 5 publications

Some observations on changes in the blood content of the cat's pancreas during activity

Some observations on changes in the blood content of the cat's pancreas during activity

Alterations of the enzyme output in ethionine pancreatitis

The actions of pancreozymin in pancreas slices and the role of phospholipids in enzyme secretion

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