The aim of this study was to evaluate the effect of enzymatic modification of poultry myofibril preparation on its selected functional properties, that is, solubility of proteins, polymerisation rates of myosin and actin, thermodynamic properties and texture. The myofibril preparation (MP) from mechanically recovered chicken meat, produced by washing and separation of fat and connective tissue, was subjected to the action of the transglutaminase preparation added at 0.3 % for a period ranging from 0.5 to 24 h at a temperature of 6-7°C. The highest dynamics of a reduction in protein solubility was observed up to 3 h of incubation. A reduction in solubility influenced the electrophoretic pattern of proteins. A significant decrease was found for the intensity of the myosin band in successive modification periods. The addition of the enzyme also influenced a reduction in enthalpy for proteins of the tested system, occurring most dynamically up to 3 h of incubation. The most distinct changes characterising a high increase in all the texture parameters of MP were found in the initial period of enzymatic modification, that is, up to 4.5 h.