The effects of Peptide Mel4-coated titanium plates on infection rabbits after internal fixation of open fractures

Zhang, Su; Zhou, Xindie; Liu, Ting; Huang, Yong; Li, Jin

doi:10.1007/s00402-020-03694-y

Cited by 9 publications

(9 citation statements)

References 16 publications

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“…For the same antimicrobial peptide, different killing mechanisms feature depending on the target bacteria involved. For instance, Mel4 induces autolysins and interferes with cell wall integrity of S. aureus, whereas with P. aeruginosa, it interacts with cell membrane lipids to result in death 56,64 .…”

Section: Advantages Of Amp Over Conventional Antibioticsmentioning

confidence: 99%

Non‐canonical amino acid bioincorporation into antimicrobial peptides and its challenges

Enninful,

Kuppusamy,

Tiburu

et al. 2024

Journal of Peptide Science

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The rise of antimicrobial resistance and multi‐drug resistant pathogens has necessitated explorations for novel antibiotic agents as the discovery of conventional antibiotics is becoming economically less viable and technically more challenging for biopharma. Antimicrobial peptides (AMPs) have emerged as a promising alternative because of their particular mode of action, broad spectrum and difficulty that microbes have in becoming resistant to them. The AMPs bacitracin, gramicidin, polymyxins and daptomycin are currently used clinically. However, their susceptibility to proteolytic degradation, toxicity profile, and complexities in large‐scale manufacture have hindered their development. To improve their proteolytic stability, methods such as integrating non‐canonical amino acids (ncAAs) into their peptide sequence have been adopted, which also improves their potency and spectrum of action. The benefits of ncAA incorporation have been made possible by solid‐phase peptide synthesis. However, this method is not always suitable for commercial production of AMPs because of poor yield, scale‐up difficulties, and its non‐‘green’ nature. Bioincorporation of ncAA as a method of integration is an emerging field geared towards tackling the challenges of solid‐phase synthesis as a green, cheaper, and scalable alternative for commercialisation of AMPs. This review focusses on the bioincorporation of ncAAs; some challenges associated with the methods are outlined, and notes are given on how to overcome these challenges. The review focusses particularly on addressing two key challenges: AMP cytotoxicity towards microbial cell factories and the uptake of ncAAs that are unfavourable to them. Overcoming these challenges will draw us closer to a greater yield and an environmentally friendly and sustainable approach to make AMPs more druggable.

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Section: Advantages Of Amp Over Conventional Antibioticsmentioning

confidence: 99%

Non‐canonical amino acid bioincorporation into antimicrobial peptides and its challenges

Enninful,

Kuppusamy,

Tiburu

et al. 2024

Journal of Peptide Science

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“…It directly co-incubates the AMPs solution with the implanted material, allowing the AMPs to physically adhere to the material surface. For example, Su et al applied Mel4 solution directly to titanium scaffolds for investigating the effectiveness of AMPs in preventing infection after internal fixation of open fractures (Zhang S et al, 2021). They inoculated rabbits with Staphylococcus aureus and immobilized the bifunctional chimeric peptide P15-CSP on the surface of tissue culture (TC) plastic by dry-coating, designing a bifunctional surface that is both antibacterial and osteogenic (Li et al, 2015).…”

Section: Surface Immobilizationmentioning

confidence: 99%

“…It directly co-incubates the AMPs solution with the implanted material, allowing the AMPs to physically adhere to the material surface. For example, Su et al applied Mel4 solution directly to titanium scaffolds for investigating the effectiveness of AMPs in preventing infection after internal fixation of open fractures ( Zhang S et al, 2021 ). They inoculated rabbits with Staphylococcus aureus and Pseudomonas aeruginosa , the common causative agents of open fractures, and implanted a titanium scaffold loaded with Mel4 into the rabbit femur to observe its therapeutic effect on postoperative infection.…”

Section: Topical Applications Of Antimicrobial Peptides For Bone Tiss...mentioning

confidence: 99%

Antimicrobial peptides for bone tissue engineering: Diversity, effects and applications

Hao

Chen²,

Chai³

et al. 2022

Front. Bioeng. Biotechnol.

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Bone tissue engineering has been becoming a promising strategy for surgical bone repair, but the risk of infection during trauma repair remains a problematic health concern worldwide, especially for fracture and infection-caused bone defects. Conventional antibiotics fail to effectively prevent or treat bone infections during bone defect repair because of drug-resistance and recurrence, so novel antibacterial agents with limited resistance are highly needed for bone tissue engineering. Antimicrobial peptides (AMPs) characterized by cationic, hydrophobic and amphipathic properties show great promise to be used as next-generation antibiotics which rarely induce resistance and show potent antibacterial efficacy. In this review, four common structures of AMPs (helix-based, sheet-based, coil-based and composite) and related modifications are presented to identify AMPs and design novel analogs. Then, potential effects of AMPs for bone infection during bone repair are explored, including bactericidal activity, anti-biofilm, immunomodulation and regenerative properties. Moreover, we present distinctive applications of AMPs for topical bone repair, which can be either used by delivery system (surface immobilization, nanoparticles and hydrogels) or used in gene therapy. Finally, future prospects and ongoing challenges are discussed.

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“…Therefore, rabbits were intravenously inoculated with bacterial suspensions of Salmonella aureus or Pseudomonas aeruginosa and the effects on Mel4 in treatment of infection after femoral fracture fixation in vivo was measured. The results showed that the AMP-coated titanium plates exhibited great potency against both tested strains, thus offering a promising way to control postoperative infections of open fractures [26]. Of interest was that coating of titanium plates was realized by simply covering the material with peptide solution, following drying under nitrogen.…”

Section: Primary Coating-use Of Specific Metal Binding Sequencesmentioning

confidence: 99%

Application of Antimicrobial Peptides on Biomedical Implants: Three Ways to Pursue Peptide Coatings

Drexelius

Neundorf

2021

IJMS

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Biofilm formation and inflammations are number one reasons of implant failure and cause a severe number of postoperative complications every year. To functionalize implant surfaces with antibiotic agents provides perspectives to minimize and/or prevent bacterial adhesion and proliferation. In recent years, antimicrobial peptides (AMP) have been evolved as promising alternatives to commonly used antibiotics, and have been seen as potent candidates for antimicrobial surface coatings. This review aims to summarize recent developments in this field and to highlight examples of the most common techniques used for preparing such AMP-based medical devices. We will report on three different ways to pursue peptide coatings, using either binding sequences (primary approach), linker layers (secondary approach), or loading in matrixes which offer a defined release (tertiary approach). All of them will be discussed in the light of current research in this area.

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The effects of Peptide Mel4-coated titanium plates on infection rabbits after internal fixation of open fractures

Cited by 9 publications

References 16 publications

Non‐canonical amino acid bioincorporation into antimicrobial peptides and its challenges

Non‐canonical amino acid bioincorporation into antimicrobial peptides and its challenges

Antimicrobial peptides for bone tissue engineering: Diversity, effects and applications

Application of Antimicrobial Peptides on Biomedical Implants: Three Ways to Pursue Peptide Coatings

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