The effects of perennial ryegrass and alfalfa on microbial abundance and diversity in petroleum contaminated soil

Kirk, Jennifer L.; Klironomos, John N.; Lee, Hung; Trevors, J. T.

doi:10.1016/j.envpol.2004.06.002

Cited by 252 publications

(121 citation statements)

References 34 publications

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“…(PAHs) (April and Sims, 1990;Siciliano et al, 2003), polychlorinated biphenyls (PCBs) (Donnelly et al, 1994), and hydrocarbons (Günther et al, 1996;Banks et al, 2003;Kirk et al, 2005). However, thus far, rhizodegradation has not been sufficiently developed for widespread application.…”

mentioning

confidence: 99%

“…The microbial community in the rhizosphere is greatly influenced by roots and root exudates that vary depending on the conditions of plant growth, such as the nutrient status (Rovira, 1959;Bowen, 1969). With regard to hydrocarboncontaminated soil, it is known that plant growth is inhibited by the toxicity of low molecular weight compounds, and that the absorption of water and nutrients by plants is limited by the hydrophobic properties of the soil (Kirk et al, 2005). To obtain t h e b a s i c fi n d i n g s r e q u i r e d f o r d e v e l o p i n g a suitable cultivation technique, we focused on the soil conditions.…”

mentioning

confidence: 99%

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Effect of Rhizodegradation in Diesel-Contaminated Soil Under Different Soil Conditions

Kaimi¹,

Mukaidani²,

Tamaki

2007

Plant Production Science

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confidence: 99%

mentioning

confidence: 99%

Effect of Rhizodegradation in Diesel-Contaminated Soil Under Different Soil Conditions

Kaimi¹,

Mukaidani²,

Tamaki

2007

Plant Production Science

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“…A contagem de células viáveis de microrganismos foi feita em placa pelo método de diluições seriadas de suspensão de solo, utilizando os meios TSA para crescimento de bactérias totais, ágar extrato de malte para fungos totais, ágar Bushnell-Hass para bactérias degradadoras de hidrocarbonetos, e meio seletivo para fungos degradadores de hidrocarbonetos (Kirk et al, 2005). Para actinomicetos totais, utilizou-se ágar amido caseína, conforme Wellington & Toth (1994).…”

Section: Avaliações Microbiológicasunclassified

“…O efeito estimulatório das raízes resulta, em parte, da modificação dos atributos físicos, químicos e biológicos do solo, estimulando as populações microbianas (Cunningham et al, 1996) a degradar contaminantes (Kirk et al, 2005). A contribuição das plantas para a eficiência do processo de remediação varia de acordo com as espécies e variedades vegetais (Siciliano et al, 2003), com as condições ambientais e propriedades do solo (Robson et al, 2003) e com as características físico-químicas do contaminante (Binet et al, 2000a).…”

Section: Introductionunclassified

Comunidades microbianas, atividade enzimática e fungos micorrízicos em solo rizosférico de "Landfarming" de resíduos petroquímicos

Nakatani

Siqueira

Soares³

et al. 2008

Rev. Bras. Ciênc. Solo

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RESUMOAs raízes das plantas podem estimular a microbiota do solo, a qual pode contribuir para o aumento da eficiência do processo de remediação. Assim, avaliar a magnitude dos efeitos das raízes sobre a microbiota do solo é de grande interesse e de relevância prática e ecológica. Neste trabalho, avaliaram-se a densidade microbiana, a atividade enzimática, a estrutura da comunidade bacteriana e a presença de fungos micorrízicos arbusculares (FMAs) na rizosfera de plantas de ocorrência espontânea em solo de sistema de "landfarming" de resíduos petroquímicos. Avaliaram-se também solos rizosféricos de cinco plantas e solocontrole sem planta por meio de contagens de microrganismos em placas, eletroforese em gel com gradiente desnaturante (DGGE) de fragmentos do gene rRNA 16S, seqüenciamento genético, atividades enzimáticas, percentagem de colonização radicular e contagem e identificação de esporos de FMAs. As plantas estimularam a densidade microbiana total e da população de degradadores de antraceno, com contagens médias de 1,5 x 10 6 e 2,2 x 10 6 UFC g -1 no solo seco, respectivamente, enquanto, no solo sem planta, essas contagens foram de 5,7 x 10 5 e 2,9 x 10 5 UFC g -1 no solo seco para os respectivos grupos microbianos. As espécies de maior efeito foram Bidens pilosa e Eclipta alba. Entretanto, esses efeitos estimulantes não foram verificados para a atividade enzimática do solo. A colonização micorrízica das raízes (em torno de 40 %) e a densidade de esporos nos solos rizosféricos foram elevadas (entre 900 e 4.800 esporos por 50 cm 3 de solo), sendo maior na Brachiaria decumbens. Foram identificadas quatro espécies de FMAs: Acaulospora morrowiae, Glomus intraradices, Paraglomus occultum e Archaeospora trappei. Com exceção de G. intraradices, essas espécies não foram

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“…The microbial population of the rhizosphere has been reported to be 4 to 100 times larger than that of the bulk soil, since the higher availability of organic matter is thought to support these high populations (Siciliano and Germida, 1998;Penrose and Glick, 2001). The composition of the microbial community is dependent on the plant species and the root exudates (Kirk et al, 2005). In addition, the microbial community may change along the roots and over time (Cook et al, 2007).…”

Section: Root Exudatesmentioning

confidence: 99%

Phytoremediation of Weathered Petroleum in Groundwater by Arroyo Willows in Nutrient Amended On-Site Mesocosms

Bragg-Flavan¹

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A large-scale mesocosm study was conducted to determine if vegetation with willow trees enhances biodegradation and to evaluate the mechanisms of natural biodegradation of weathered petroleum compounds under field conditions. The mesocosms were designed to model conditions at a former oil field where mid-range petroleum distillates were used as a diluent for pumping crude oil contaminated the soil and groundwater at the site with petroleum compounds. Ten mesocosms were constructed at the field site using un-impacted soil and diluent-impacted groundwater from the site. Five of the mesocosms were planted with Arroyo Willow trees native to the field site and the other five served as controls without trees. Since these willow trees are phreatophytes, their roots are capable of consuming water from the water table. A previous study was conducted using these mesocosms, however the willow trees then were in poor condition.In this study, fertilizer was added to the mesocosms to promote healthy growth of the willows. Fertilizer was added equally to mesocosms with and without willow trees to avoid introducing bias. Groundwater was circulated through the mesocosms for two 109 to 126 days runs, while the total petroleum hydrocarbon (TPH) concentrations of the groundwater were measured periodically. Dissolved oxygen concentrations were also monitored in each of the mesocosms to determine if the willow trees had any impact on oxygen transfer to the subsurface.In the first run without nutrient amendments the trees did not enhance biodegradation.All the mesocosms started with an average TPH concentration of 6.3 mg/L and ended vi with a concentration of 1.0 mg/L. After this first run, nutrient amendments were added to all the mesocosms, resulting in healthy trees with robust growth. With healthy willow trees, the planted mesocosms resulted in a statistically significant increase in long-term biodegradation of dissolved-phase petroleum compounds. The planted mesocosms resulted in 29 percent more degradation. These results agree with prior lab studies using bench-scale microcosms with media from the former oil field which indicated that TPH concentrations after 100 days were lower in containers with willows or lupines compared to controls without plants. Microtox ® toxicity decreased for both planted and control mesocosms, showing no toxic root exudates or by-products.There are several potential mechanisms of the observed phytoremediation. Terminal restriction fragment analyses showed that the planted mesocosms had different microbial communities than the unplanted mesocosms. Thus, a possible mechanism of the phytoremediation is stimulation of a rhizobial microbial community that biodegrades petroleum compounds. The dissolved oxygen (DO) concentrations were actually lower in the planted mesocosms, possibly due to consumption of oxygen during biodegradation of root exudates. The reduced DO concentrations in the planted mesocosm discounts the possibility that the plants stimulated biodegradation by increasing oxygen transfer to the ...

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The effects of perennial ryegrass and alfalfa on microbial abundance and diversity in petroleum contaminated soil

Cited by 252 publications

References 34 publications

Effect of Rhizodegradation in Diesel-Contaminated Soil Under Different Soil Conditions

Effect of Rhizodegradation in Diesel-Contaminated Soil Under Different Soil Conditions

Comunidades microbianas, atividade enzimática e fungos micorrízicos em solo rizosférico de "Landfarming" de resíduos petroquímicos

Phytoremediation of Weathered Petroleum in Groundwater by Arroyo Willows in Nutrient Amended On-Site Mesocosms

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