The effects of pH, osmolarity and urine contamination on equine spermatozoal motility

Griggers, S.; Paccamonti, Dale L.; Thompson, R. A.; Eilts, B.E.

doi:10.1016/s0093-691x(01)00593-3

Cited by 80 publications

(43 citation statements)

References 10 publications

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“…Urospermia causes deleterious effects on semen in several species and these effects are due to changes in pH and osmolality and to the toxic effect of urine components (Griggers et al, 2001). Sensitivity of spermatozoa to osmotic damage differs markedly among species and this determines the ability of cells to recover motility after osmotic stress.…”

Section: Discussionmentioning

confidence: 99%

“…The deleterious effects of urine on spermatozoa are due to changes in pH and osmolality, as well as to the toxic effect of urea and other urine components (Griggers et al, 2001;Wirtu et al, 2008). Urine contamination was detrimental to sperm motility (Kim and Kim, 1998) and membrane and acrosomal integrity (Santos et al, 2011), and reduces the sperm fertilizing capacity (Santos et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…In horses, the recovery of progressive sperm motility, reduced by urine contamination, depends on the extender used to dilute semen (Griggers et al, 2001). Human spermatozoa survival exposed to hyperosmotic solutions was affected by temperature and by time of exposure to anisosmotic environments (Gao et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Salvaging urospermic ejaculates from brown bear (Ursus arctos)

Gomes-Alves

Álvarez

Nicolás

et al. 2014

Animal Reproduction Science

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Salvaging urospermic ejaculates from brown bear (Ursus arctos)

Gomes-Alves

Álvarez

Nicolás

et al. 2014

Animal Reproduction Science

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“…However, MILLER et al (2001) reported that T. m. latirostris spermatozoa show a very pronounced annulus, and this structure was not observed in T. inunguis. On the other hand, the alterations observed (swelling and irregular shape of acrosome and the disruption or loss of plasmatic membrane) probably are post-mortem degeneration due to osmotic stress by the urine (VIJAYAKUMAR et al 1986, GRIGGERS et al 2001. Therefore, the annulus from Amazonian manatee spermatozoa might have been lost.…”

Section: Shortmentioning

confidence: 99%

Morphology, morphometry and ultrastructure of the Amazonian manatee (Sirenia: Trichechidae) spermatozoa

Amaral

Lucci

Rosas

et al. 2010

Zoologia (Curitiba, Impr.)

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This study describes the morphological, morphometric and ultrastructural characteristics of the Amazonian manatee Trichechus inunguis (Natterer, 1883) spermatozoon. The spermatozoa were obtained from a urine sample of an adult T. inunguis kept in captivity. The spermatozoa were analyzed by light and transmission electron microscopy. The head of Amazonian manatee spermatozoa had a flat oval shape and a well distinguishable midpiece. The mean dimensions of the spermatozoa were: head length, 7.49 ± 0.24 µm; head width, 3.53 ± 0.19 µm; head thickness, 1.61 ± 0.13 µm; midpiece length, 11.36 ± 0.34 µm; flagellum length, 40.91 ± 1.94 µm; total tail length, 52.16 ± 1.06 µm; total spermatozoon length, 60.08 ± 1.40 µm. The Amazonian manatee spermatozoa were similar in shape to other sirenian spermatozoa; however, presenting a different size. This study describes, for the first time, the morphometric and ultrastructural characteristics of the Amazonian manatee spermatozoa, and also demonstrates the possible use of spermatozoa retrieved from urine samples for biological studies

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“…Centrifugation is one of these procedures and found to be an essential step in the cryopreservation protocol of some species, either to concentrate diluted samples (bear, Ishikawa et al 2002;Kojima et al 2001), or to wash urine-contaminated semen (horse, Griggers et al 2001;human, Makler et al 1981;Kim and Kim 1998). Moreover, some authors propose the semen centrifugation to remove the effects deleterious of seminal plasma (dog, Rota et al 1995;goat, Pellicer-Rubio et al 1997;ram, Ritar and Salamon 1982;stallion, Jasko et al 1991;Carver and Ball 2002;Sieme et al 2004;bull, Way et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Spermatozoa recovery and post-thawing quality of brown bear ejaculates is affected for centrifugation regimes

et al. 2011

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Sperm cryopreservation protocols for brown bear (Ursus arctos) require the centrifugation of semen samples to increase sperm concentration and to clean urine in contaminated samples. We evaluated the effect of centrifugation regimes (time and relative centrifugal force-RCF) on the quantity of sperm recovered and the quality of postthawed sperm. Thirteen brown bears were electroejaculated. The ejaculates were diluted 1:1 in Tris-citric acid-glucose (TCG) extender and centrifuged with different RCF/time combinations: 600×g, 1,200×g and 2,400×g, for 3, 6 or 12 min. After centrifugation, spermatozoa were diluted in TES-Tris-fructose extender with egg yolk and glycerol (final glycerol concentration of 8%) and frozen in 0.25-mL straws. In the post-thawed semen, motility was assessed by CASA, and acrosomal status (PNA-FITC), viability (SYBR-14 with propidium iodide) and chromatin status (SCSA) were determined by flow cytometry. The longest centrifugation time (12 min) significantly decreased some motility parameters. Sperm recovery significantly decreased in brown bear at 600×g. Our results suggest that brown bear spermatozoa are more sensitive to long centrifugation times than to high RCF. Centrifugation regimes showed no effects on the post-thawing chromatin status. We recommend preparing the brown bear semen for freezing by centrifugation 1,200×g or 2,400×g for 6 min, after electroejaculation and dilution 1:1 in TCG extender, since these procedures increase the spermatozoa recovery without harmful effects on the post-thawed quality of brown bear spermatozoa.

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The effects of pH, osmolarity and urine contamination on equine spermatozoal motility

Cited by 80 publications

References 10 publications

Salvaging urospermic ejaculates from brown bear (Ursus arctos)

Salvaging urospermic ejaculates from brown bear (Ursus arctos)

Morphology, morphometry and ultrastructure of the Amazonian manatee (Sirenia: Trichechidae) spermatozoa

Spermatozoa recovery and post-thawing quality of brown bear ejaculates is affected for centrifugation regimes

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